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Enhancement of bone consolidation using high-frequency pulsed electromagnetic short-waves and titanium implants coated with biomimetic composite embedded into PLA matrix: in vivo evaluation

PURPOSE: Bone consolidation after severe trauma is the most challenging task in orthopedic surgery. This study aimed to develop biomimetic composite for coating Ti implants. Afterwards, these implants were tested in vivo to assess bone consolidation in the absence or the presence of high-frequency p...

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Autores principales: Oltean-Dan, Daniel, Dogaru, Gabriela-Bombonica, Tomoaia-Cotisel, Maria, Apostu, Dragos, Mester, Alexandru, Benea, Horea-Rares-Ciprian, Paiusan, Mihai-Gheorghe, Jianu, Elena-Mihaela, Mocanu, Aurora, Balint, Reka, Popa, Catalin-Ovidiu, Berce, Cristian, Bodizs, Gyorgy-Istvan, Toader, Alina-Mihaela, Tomoaia, Gheorghe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6664427/
https://www.ncbi.nlm.nih.gov/pubmed/31440048
http://dx.doi.org/10.2147/IJN.S205880
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author Oltean-Dan, Daniel
Dogaru, Gabriela-Bombonica
Tomoaia-Cotisel, Maria
Apostu, Dragos
Mester, Alexandru
Benea, Horea-Rares-Ciprian
Paiusan, Mihai-Gheorghe
Jianu, Elena-Mihaela
Mocanu, Aurora
Balint, Reka
Popa, Catalin-Ovidiu
Berce, Cristian
Bodizs, Gyorgy-Istvan
Toader, Alina-Mihaela
Tomoaia, Gheorghe
author_facet Oltean-Dan, Daniel
Dogaru, Gabriela-Bombonica
Tomoaia-Cotisel, Maria
Apostu, Dragos
Mester, Alexandru
Benea, Horea-Rares-Ciprian
Paiusan, Mihai-Gheorghe
Jianu, Elena-Mihaela
Mocanu, Aurora
Balint, Reka
Popa, Catalin-Ovidiu
Berce, Cristian
Bodizs, Gyorgy-Istvan
Toader, Alina-Mihaela
Tomoaia, Gheorghe
author_sort Oltean-Dan, Daniel
collection PubMed
description PURPOSE: Bone consolidation after severe trauma is the most challenging task in orthopedic surgery. This study aimed to develop biomimetic composite for coating Ti implants. Afterwards, these implants were tested in vivo to assess bone consolidation in the absence or the presence of high-frequency pulsed electromagnetic short-waves (HF-PESW). MATERIALS: Biomimetic coating was successfully developed using multi-substituted hydroxyapatite (ms-HAP) functionalized with collagen (ms-HAP/COL), embedded into poly-lactic acid (PLA) matrix (ms-HAP/COL@PLA), and subsequently covered with self-assembled COL layer (ms-HAP/COL@PLA/COL, named HAPc). METHODS: For in vivo evaluation, 32 Wistar albino rats were used in four groups: control group (CG) with Ti implant; PESW group with Ti implant+HF-PESW; HAPc group with Ti implant coated with HAPc; HAPc+PESW group with Ti implant coated with HAPc+HF-PESW. Left femoral diaphysis was fractured and fixed intramedullary. From the first post-operative day, PESW and HAPc+PESW groups underwent HF-PESW stimulation for 14 consecutive days. Biomimetic coating was characterized by XRD, HR-TEM, SEM, EDX and AFM. RESULTS: Osteogenic markers (ALP and osteocalcin) and micro-computed tomography (CT) analysis (especially bone volume/tissue volume ratio results) indicated at 2 weeks the following group order: HAPc+PESW>HAPc≈PESW (P>0.05) and HAPc+PESW>control (P<0.05), indicating the higher values in HAPc+PESW group compared to CG. The fracture-site bone strength showed, at 2 weeks, the highest average value in HAPc+PESW group. Moreover, histological analysis revealed the most abundant COL fibers assembled in dense bundles in HAPc-PESW group. At 8 weeks, micro-CT indicated higher values only in HAPc+PESW group vs CG (P<0.05), and histological results showed a complete-healed fracture in groups: HAPc+PESW, HAPc and PESW, but with more advanced bone remodeling in HAPc+PESW group. CONCLUSION: Using Ti implants coated by HAPc jointly with HF-PESW stimulation positively influenced the bone consolidation process, especially in its early phase, thus potentially providing a superior strategy for clinical applications.
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spelling pubmed-66644272019-08-22 Enhancement of bone consolidation using high-frequency pulsed electromagnetic short-waves and titanium implants coated with biomimetic composite embedded into PLA matrix: in vivo evaluation Oltean-Dan, Daniel Dogaru, Gabriela-Bombonica Tomoaia-Cotisel, Maria Apostu, Dragos Mester, Alexandru Benea, Horea-Rares-Ciprian Paiusan, Mihai-Gheorghe Jianu, Elena-Mihaela Mocanu, Aurora Balint, Reka Popa, Catalin-Ovidiu Berce, Cristian Bodizs, Gyorgy-Istvan Toader, Alina-Mihaela Tomoaia, Gheorghe Int J Nanomedicine Original Research PURPOSE: Bone consolidation after severe trauma is the most challenging task in orthopedic surgery. This study aimed to develop biomimetic composite for coating Ti implants. Afterwards, these implants were tested in vivo to assess bone consolidation in the absence or the presence of high-frequency pulsed electromagnetic short-waves (HF-PESW). MATERIALS: Biomimetic coating was successfully developed using multi-substituted hydroxyapatite (ms-HAP) functionalized with collagen (ms-HAP/COL), embedded into poly-lactic acid (PLA) matrix (ms-HAP/COL@PLA), and subsequently covered with self-assembled COL layer (ms-HAP/COL@PLA/COL, named HAPc). METHODS: For in vivo evaluation, 32 Wistar albino rats were used in four groups: control group (CG) with Ti implant; PESW group with Ti implant+HF-PESW; HAPc group with Ti implant coated with HAPc; HAPc+PESW group with Ti implant coated with HAPc+HF-PESW. Left femoral diaphysis was fractured and fixed intramedullary. From the first post-operative day, PESW and HAPc+PESW groups underwent HF-PESW stimulation for 14 consecutive days. Biomimetic coating was characterized by XRD, HR-TEM, SEM, EDX and AFM. RESULTS: Osteogenic markers (ALP and osteocalcin) and micro-computed tomography (CT) analysis (especially bone volume/tissue volume ratio results) indicated at 2 weeks the following group order: HAPc+PESW>HAPc≈PESW (P>0.05) and HAPc+PESW>control (P<0.05), indicating the higher values in HAPc+PESW group compared to CG. The fracture-site bone strength showed, at 2 weeks, the highest average value in HAPc+PESW group. Moreover, histological analysis revealed the most abundant COL fibers assembled in dense bundles in HAPc-PESW group. At 8 weeks, micro-CT indicated higher values only in HAPc+PESW group vs CG (P<0.05), and histological results showed a complete-healed fracture in groups: HAPc+PESW, HAPc and PESW, but with more advanced bone remodeling in HAPc+PESW group. CONCLUSION: Using Ti implants coated by HAPc jointly with HF-PESW stimulation positively influenced the bone consolidation process, especially in its early phase, thus potentially providing a superior strategy for clinical applications. Dove 2019-07-25 /pmc/articles/PMC6664427/ /pubmed/31440048 http://dx.doi.org/10.2147/IJN.S205880 Text en © 2019 Oltean-Dan et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Oltean-Dan, Daniel
Dogaru, Gabriela-Bombonica
Tomoaia-Cotisel, Maria
Apostu, Dragos
Mester, Alexandru
Benea, Horea-Rares-Ciprian
Paiusan, Mihai-Gheorghe
Jianu, Elena-Mihaela
Mocanu, Aurora
Balint, Reka
Popa, Catalin-Ovidiu
Berce, Cristian
Bodizs, Gyorgy-Istvan
Toader, Alina-Mihaela
Tomoaia, Gheorghe
Enhancement of bone consolidation using high-frequency pulsed electromagnetic short-waves and titanium implants coated with biomimetic composite embedded into PLA matrix: in vivo evaluation
title Enhancement of bone consolidation using high-frequency pulsed electromagnetic short-waves and titanium implants coated with biomimetic composite embedded into PLA matrix: in vivo evaluation
title_full Enhancement of bone consolidation using high-frequency pulsed electromagnetic short-waves and titanium implants coated with biomimetic composite embedded into PLA matrix: in vivo evaluation
title_fullStr Enhancement of bone consolidation using high-frequency pulsed electromagnetic short-waves and titanium implants coated with biomimetic composite embedded into PLA matrix: in vivo evaluation
title_full_unstemmed Enhancement of bone consolidation using high-frequency pulsed electromagnetic short-waves and titanium implants coated with biomimetic composite embedded into PLA matrix: in vivo evaluation
title_short Enhancement of bone consolidation using high-frequency pulsed electromagnetic short-waves and titanium implants coated with biomimetic composite embedded into PLA matrix: in vivo evaluation
title_sort enhancement of bone consolidation using high-frequency pulsed electromagnetic short-waves and titanium implants coated with biomimetic composite embedded into pla matrix: in vivo evaluation
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6664427/
https://www.ncbi.nlm.nih.gov/pubmed/31440048
http://dx.doi.org/10.2147/IJN.S205880
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