Cargando…
Characterization of CMY-2-type beta-lactamase-producing Escherichia coli isolated from chicken carcasses and human infection in a city of South Brazil
BACKGROUND: Food-producing animals, mainly poultry, have been associated with the maintenance and dissemination of antibiotic-resistant bacteria, such as plasmid-mediated AmpC (pAmpC)-producing Enterobacteriaceae, to humans, thus impacting food safety. Many studies have shown that Escherichia coli s...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6664532/ https://www.ncbi.nlm.nih.gov/pubmed/31362706 http://dx.doi.org/10.1186/s12866-019-1550-3 |
_version_ | 1783439903322800128 |
---|---|
author | Koga, Vanessa L. Maluta, Renato P. da Silveira, Wanderley D. Ribeiro, Renan A. Hungria, Mariangela Vespero, Eliana C. Nakazato, Gerson Kobayashi, Renata K. T. |
author_facet | Koga, Vanessa L. Maluta, Renato P. da Silveira, Wanderley D. Ribeiro, Renan A. Hungria, Mariangela Vespero, Eliana C. Nakazato, Gerson Kobayashi, Renata K. T. |
author_sort | Koga, Vanessa L. |
collection | PubMed |
description | BACKGROUND: Food-producing animals, mainly poultry, have been associated with the maintenance and dissemination of antibiotic-resistant bacteria, such as plasmid-mediated AmpC (pAmpC)-producing Enterobacteriaceae, to humans, thus impacting food safety. Many studies have shown that Escherichia coli strains isolated from poultry and humans infections share identical cephalosporin resistance, suggesting that transmission of resistance from poultry meat to humans may occur. The aim of this study was to characterize pAmpC-producing E. coli strains isolated from chicken carcasses and human infection in a restrict area and to determine their antimicrobial resistance profiles, and molecular type by multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). RESULTS: A total of 14 pAmpC-producing E. coli strains were isolated, including eight strains from chicken carcasses and six strains from human infections (from urine, tissue and secretion). The bla(CMY-2) gene was identified in all pAmpC-producing E. coli strains by polymerase chain reaction (PCR) and DNA sequencing. High percentages of strains resistant to tetracycline, nalidixic acid and sulfamethoxazole-trimethoprim (78–92%) were detected, all of which were considered multidrug-resistant. Among the non-beta-lactam resistance genes, the majority of the strains showed tetA, tetB, sulI and sulII. No strain was considered an extended-spectrum beta-lactamases (ESBL) producer, and the bla(TEM-1) gene was found in 2 strains isolated from human infection. Six strains from chicken carcasses and four strains from humans infections were linked to an ISEcp1-like element. Through MLST, 11 sequence types were found. Three strains isolated from human infection and one strain isolated from chicken carcasses belonged to the same sequence type (ST354). However, considerable heterogeneity between the strains from chicken carcasses and humans was confirmed by PFGE analysis. CONCLUSION: This study showed the prevalence of E. coli strains producing bla(CMY-2) linked to ISEcp1 that were present in both chickens and humans in a restricted area. Our results also suggest the presence of a highly diverse strains that harbor pAmpC, indicating no clonal dissemination. Therefore, continuous monitoring and comparative analyses of resistant bacteria from humans and food-producing animals are needed. |
format | Online Article Text |
id | pubmed-6664532 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-66645322019-08-05 Characterization of CMY-2-type beta-lactamase-producing Escherichia coli isolated from chicken carcasses and human infection in a city of South Brazil Koga, Vanessa L. Maluta, Renato P. da Silveira, Wanderley D. Ribeiro, Renan A. Hungria, Mariangela Vespero, Eliana C. Nakazato, Gerson Kobayashi, Renata K. T. BMC Microbiol Research Article BACKGROUND: Food-producing animals, mainly poultry, have been associated with the maintenance and dissemination of antibiotic-resistant bacteria, such as plasmid-mediated AmpC (pAmpC)-producing Enterobacteriaceae, to humans, thus impacting food safety. Many studies have shown that Escherichia coli strains isolated from poultry and humans infections share identical cephalosporin resistance, suggesting that transmission of resistance from poultry meat to humans may occur. The aim of this study was to characterize pAmpC-producing E. coli strains isolated from chicken carcasses and human infection in a restrict area and to determine their antimicrobial resistance profiles, and molecular type by multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). RESULTS: A total of 14 pAmpC-producing E. coli strains were isolated, including eight strains from chicken carcasses and six strains from human infections (from urine, tissue and secretion). The bla(CMY-2) gene was identified in all pAmpC-producing E. coli strains by polymerase chain reaction (PCR) and DNA sequencing. High percentages of strains resistant to tetracycline, nalidixic acid and sulfamethoxazole-trimethoprim (78–92%) were detected, all of which were considered multidrug-resistant. Among the non-beta-lactam resistance genes, the majority of the strains showed tetA, tetB, sulI and sulII. No strain was considered an extended-spectrum beta-lactamases (ESBL) producer, and the bla(TEM-1) gene was found in 2 strains isolated from human infection. Six strains from chicken carcasses and four strains from humans infections were linked to an ISEcp1-like element. Through MLST, 11 sequence types were found. Three strains isolated from human infection and one strain isolated from chicken carcasses belonged to the same sequence type (ST354). However, considerable heterogeneity between the strains from chicken carcasses and humans was confirmed by PFGE analysis. CONCLUSION: This study showed the prevalence of E. coli strains producing bla(CMY-2) linked to ISEcp1 that were present in both chickens and humans in a restricted area. Our results also suggest the presence of a highly diverse strains that harbor pAmpC, indicating no clonal dissemination. Therefore, continuous monitoring and comparative analyses of resistant bacteria from humans and food-producing animals are needed. BioMed Central 2019-07-30 /pmc/articles/PMC6664532/ /pubmed/31362706 http://dx.doi.org/10.1186/s12866-019-1550-3 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Koga, Vanessa L. Maluta, Renato P. da Silveira, Wanderley D. Ribeiro, Renan A. Hungria, Mariangela Vespero, Eliana C. Nakazato, Gerson Kobayashi, Renata K. T. Characterization of CMY-2-type beta-lactamase-producing Escherichia coli isolated from chicken carcasses and human infection in a city of South Brazil |
title | Characterization of CMY-2-type beta-lactamase-producing Escherichia coli isolated from chicken carcasses and human infection in a city of South Brazil |
title_full | Characterization of CMY-2-type beta-lactamase-producing Escherichia coli isolated from chicken carcasses and human infection in a city of South Brazil |
title_fullStr | Characterization of CMY-2-type beta-lactamase-producing Escherichia coli isolated from chicken carcasses and human infection in a city of South Brazil |
title_full_unstemmed | Characterization of CMY-2-type beta-lactamase-producing Escherichia coli isolated from chicken carcasses and human infection in a city of South Brazil |
title_short | Characterization of CMY-2-type beta-lactamase-producing Escherichia coli isolated from chicken carcasses and human infection in a city of South Brazil |
title_sort | characterization of cmy-2-type beta-lactamase-producing escherichia coli isolated from chicken carcasses and human infection in a city of south brazil |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6664532/ https://www.ncbi.nlm.nih.gov/pubmed/31362706 http://dx.doi.org/10.1186/s12866-019-1550-3 |
work_keys_str_mv | AT kogavanessal characterizationofcmy2typebetalactamaseproducingescherichiacoliisolatedfromchickencarcassesandhumaninfectioninacityofsouthbrazil AT malutarenatop characterizationofcmy2typebetalactamaseproducingescherichiacoliisolatedfromchickencarcassesandhumaninfectioninacityofsouthbrazil AT dasilveirawanderleyd characterizationofcmy2typebetalactamaseproducingescherichiacoliisolatedfromchickencarcassesandhumaninfectioninacityofsouthbrazil AT ribeirorenana characterizationofcmy2typebetalactamaseproducingescherichiacoliisolatedfromchickencarcassesandhumaninfectioninacityofsouthbrazil AT hungriamariangela characterizationofcmy2typebetalactamaseproducingescherichiacoliisolatedfromchickencarcassesandhumaninfectioninacityofsouthbrazil AT vesperoelianac characterizationofcmy2typebetalactamaseproducingescherichiacoliisolatedfromchickencarcassesandhumaninfectioninacityofsouthbrazil AT nakazatogerson characterizationofcmy2typebetalactamaseproducingescherichiacoliisolatedfromchickencarcassesandhumaninfectioninacityofsouthbrazil AT kobayashirenatakt characterizationofcmy2typebetalactamaseproducingescherichiacoliisolatedfromchickencarcassesandhumaninfectioninacityofsouthbrazil |