Cargando…
Human Cytomegalovirus Genomes Sequenced Directly From Clinical Material: Variation, Multiple-Strain Infection, Recombination, and Gene Loss
The genomic characteristics of human cytomegalovirus (HCMV) strains sequenced directly from clinical pathology samples were investigated, focusing on variation, multiple-strain infection, recombination, and gene loss. A total of 207 datasets generated in this and previous studies using target enrich...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6667795/ https://www.ncbi.nlm.nih.gov/pubmed/31050742 http://dx.doi.org/10.1093/infdis/jiz208 |
_version_ | 1783440099812311040 |
---|---|
author | Suárez, Nicolás M Wilkie, Gavin S Hage, Elias Camiolo, Salvatore Holton, Marylouisa Hughes, Joseph Maabar, Maha Vattipally, Sreenu B Dhingra, Akshay Gompels, Ursula A Wilkinson, Gavin W G Baldanti, Fausto Furione, Milena Lilleri, Daniele Arossa, Alessia Ganzenmueller, Tina Gerna, Giuseppe Hubáček, Petr Schulz, Thomas F Wolf, Dana Zavattoni, Maurizio Davison, Andrew J |
author_facet | Suárez, Nicolás M Wilkie, Gavin S Hage, Elias Camiolo, Salvatore Holton, Marylouisa Hughes, Joseph Maabar, Maha Vattipally, Sreenu B Dhingra, Akshay Gompels, Ursula A Wilkinson, Gavin W G Baldanti, Fausto Furione, Milena Lilleri, Daniele Arossa, Alessia Ganzenmueller, Tina Gerna, Giuseppe Hubáček, Petr Schulz, Thomas F Wolf, Dana Zavattoni, Maurizio Davison, Andrew J |
author_sort | Suárez, Nicolás M |
collection | PubMed |
description | The genomic characteristics of human cytomegalovirus (HCMV) strains sequenced directly from clinical pathology samples were investigated, focusing on variation, multiple-strain infection, recombination, and gene loss. A total of 207 datasets generated in this and previous studies using target enrichment and high-throughput sequencing were analyzed, in the process enabling the determination of genome sequences for 91 strains. Key findings were that (i) it is important to monitor the quality of sequencing libraries in investigating variation; (ii) many recombinant strains have been transmitted during HCMV evolution, and some have apparently survived for thousands of years without further recombination; (iii) mutants with nonfunctional genes (pseudogenes) have been circulating and recombining for long periods and can cause congenital infection and resulting clinical sequelae; and (iv) intrahost variation in single-strain infections is much less than that in multiple-strain infections. Future population-based studies are likely to continue illuminating the evolution, epidemiology, and pathogenesis of HCMV. |
format | Online Article Text |
id | pubmed-6667795 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-66677952019-08-05 Human Cytomegalovirus Genomes Sequenced Directly From Clinical Material: Variation, Multiple-Strain Infection, Recombination, and Gene Loss Suárez, Nicolás M Wilkie, Gavin S Hage, Elias Camiolo, Salvatore Holton, Marylouisa Hughes, Joseph Maabar, Maha Vattipally, Sreenu B Dhingra, Akshay Gompels, Ursula A Wilkinson, Gavin W G Baldanti, Fausto Furione, Milena Lilleri, Daniele Arossa, Alessia Ganzenmueller, Tina Gerna, Giuseppe Hubáček, Petr Schulz, Thomas F Wolf, Dana Zavattoni, Maurizio Davison, Andrew J J Infect Dis Major Articles and Brief Reports The genomic characteristics of human cytomegalovirus (HCMV) strains sequenced directly from clinical pathology samples were investigated, focusing on variation, multiple-strain infection, recombination, and gene loss. A total of 207 datasets generated in this and previous studies using target enrichment and high-throughput sequencing were analyzed, in the process enabling the determination of genome sequences for 91 strains. Key findings were that (i) it is important to monitor the quality of sequencing libraries in investigating variation; (ii) many recombinant strains have been transmitted during HCMV evolution, and some have apparently survived for thousands of years without further recombination; (iii) mutants with nonfunctional genes (pseudogenes) have been circulating and recombining for long periods and can cause congenital infection and resulting clinical sequelae; and (iv) intrahost variation in single-strain infections is much less than that in multiple-strain infections. Future population-based studies are likely to continue illuminating the evolution, epidemiology, and pathogenesis of HCMV. Oxford University Press 2019-09-01 2019-05-02 /pmc/articles/PMC6667795/ /pubmed/31050742 http://dx.doi.org/10.1093/infdis/jiz208 Text en © The Author(s) 2019. Published by Oxford University Press for the Infectious Diseases Society of America. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Major Articles and Brief Reports Suárez, Nicolás M Wilkie, Gavin S Hage, Elias Camiolo, Salvatore Holton, Marylouisa Hughes, Joseph Maabar, Maha Vattipally, Sreenu B Dhingra, Akshay Gompels, Ursula A Wilkinson, Gavin W G Baldanti, Fausto Furione, Milena Lilleri, Daniele Arossa, Alessia Ganzenmueller, Tina Gerna, Giuseppe Hubáček, Petr Schulz, Thomas F Wolf, Dana Zavattoni, Maurizio Davison, Andrew J Human Cytomegalovirus Genomes Sequenced Directly From Clinical Material: Variation, Multiple-Strain Infection, Recombination, and Gene Loss |
title | Human Cytomegalovirus Genomes Sequenced Directly From Clinical Material: Variation, Multiple-Strain Infection, Recombination, and Gene Loss |
title_full | Human Cytomegalovirus Genomes Sequenced Directly From Clinical Material: Variation, Multiple-Strain Infection, Recombination, and Gene Loss |
title_fullStr | Human Cytomegalovirus Genomes Sequenced Directly From Clinical Material: Variation, Multiple-Strain Infection, Recombination, and Gene Loss |
title_full_unstemmed | Human Cytomegalovirus Genomes Sequenced Directly From Clinical Material: Variation, Multiple-Strain Infection, Recombination, and Gene Loss |
title_short | Human Cytomegalovirus Genomes Sequenced Directly From Clinical Material: Variation, Multiple-Strain Infection, Recombination, and Gene Loss |
title_sort | human cytomegalovirus genomes sequenced directly from clinical material: variation, multiple-strain infection, recombination, and gene loss |
topic | Major Articles and Brief Reports |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6667795/ https://www.ncbi.nlm.nih.gov/pubmed/31050742 http://dx.doi.org/10.1093/infdis/jiz208 |
work_keys_str_mv | AT suareznicolasm humancytomegalovirusgenomessequenceddirectlyfromclinicalmaterialvariationmultiplestraininfectionrecombinationandgeneloss AT wilkiegavins humancytomegalovirusgenomessequenceddirectlyfromclinicalmaterialvariationmultiplestraininfectionrecombinationandgeneloss AT hageelias humancytomegalovirusgenomessequenceddirectlyfromclinicalmaterialvariationmultiplestraininfectionrecombinationandgeneloss AT camiolosalvatore humancytomegalovirusgenomessequenceddirectlyfromclinicalmaterialvariationmultiplestraininfectionrecombinationandgeneloss AT holtonmarylouisa humancytomegalovirusgenomessequenceddirectlyfromclinicalmaterialvariationmultiplestraininfectionrecombinationandgeneloss AT hughesjoseph humancytomegalovirusgenomessequenceddirectlyfromclinicalmaterialvariationmultiplestraininfectionrecombinationandgeneloss AT maabarmaha humancytomegalovirusgenomessequenceddirectlyfromclinicalmaterialvariationmultiplestraininfectionrecombinationandgeneloss AT vattipallysreenub humancytomegalovirusgenomessequenceddirectlyfromclinicalmaterialvariationmultiplestraininfectionrecombinationandgeneloss AT dhingraakshay humancytomegalovirusgenomessequenceddirectlyfromclinicalmaterialvariationmultiplestraininfectionrecombinationandgeneloss AT gompelsursulaa humancytomegalovirusgenomessequenceddirectlyfromclinicalmaterialvariationmultiplestraininfectionrecombinationandgeneloss AT wilkinsongavinwg humancytomegalovirusgenomessequenceddirectlyfromclinicalmaterialvariationmultiplestraininfectionrecombinationandgeneloss AT baldantifausto humancytomegalovirusgenomessequenceddirectlyfromclinicalmaterialvariationmultiplestraininfectionrecombinationandgeneloss AT furionemilena humancytomegalovirusgenomessequenceddirectlyfromclinicalmaterialvariationmultiplestraininfectionrecombinationandgeneloss AT lilleridaniele humancytomegalovirusgenomessequenceddirectlyfromclinicalmaterialvariationmultiplestraininfectionrecombinationandgeneloss AT arossaalessia humancytomegalovirusgenomessequenceddirectlyfromclinicalmaterialvariationmultiplestraininfectionrecombinationandgeneloss AT ganzenmuellertina humancytomegalovirusgenomessequenceddirectlyfromclinicalmaterialvariationmultiplestraininfectionrecombinationandgeneloss AT gernagiuseppe humancytomegalovirusgenomessequenceddirectlyfromclinicalmaterialvariationmultiplestraininfectionrecombinationandgeneloss AT hubacekpetr humancytomegalovirusgenomessequenceddirectlyfromclinicalmaterialvariationmultiplestraininfectionrecombinationandgeneloss AT schulzthomasf humancytomegalovirusgenomessequenceddirectlyfromclinicalmaterialvariationmultiplestraininfectionrecombinationandgeneloss AT wolfdana humancytomegalovirusgenomessequenceddirectlyfromclinicalmaterialvariationmultiplestraininfectionrecombinationandgeneloss AT zavattonimaurizio humancytomegalovirusgenomessequenceddirectlyfromclinicalmaterialvariationmultiplestraininfectionrecombinationandgeneloss AT davisonandrewj humancytomegalovirusgenomessequenceddirectlyfromclinicalmaterialvariationmultiplestraininfectionrecombinationandgeneloss |