Cargando…
Comparative analysis of CDR3 regions in paired human αβ CD8 T cells
The majority of human CD8 cytotoxic T lymphocytes express αβ T‐cell receptors that recognize peptide–MHC class I complexes. Considerable attention has been devoted to TCR β repertoires, but study of TCR α chains has been limited. To gain a better understanding of the features of CDR3α and CDR3β in p...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6668380/ https://www.ncbi.nlm.nih.gov/pubmed/31237075 http://dx.doi.org/10.1002/2211-5463.12690 |
_version_ | 1783440202959683584 |
---|---|
author | Yu, Kun Shi, Ji Lu, Dan Yang, Qiong |
author_facet | Yu, Kun Shi, Ji Lu, Dan Yang, Qiong |
author_sort | Yu, Kun |
collection | PubMed |
description | The majority of human CD8 cytotoxic T lymphocytes express αβ T‐cell receptors that recognize peptide–MHC class I complexes. Considerable attention has been devoted to TCR β repertoires, but study of TCR α chains has been limited. To gain a better understanding of the features of CDR3α and CDR3β in paired samples, we comprehensively analyzed 776 unique paired αβ TCR CDR3 regions in this study. We found that (I) the CDR3 length among paired αβ TCRs had a fairly narrow distribution due to random assortment of CDR3 length in alpha and beta chains; (II) nucleotide deletions among CDR3 regions were positively correlated with insertions in both α and β TCRs; (III) the CDR3 loops of both α and β chains contained an abundance of charged/polar residues and the CDR3 base regions contained a conserved motif; and (IV) the occurrence of Gly was CDR3 length‐ and position‐dependent in both chains, whereas the frequency of Ser at positions 106 and 107 was positively correlated with CDR3 length in TCR β. Overall, the amino acids in CDR3 loop regions were significantly different between TCR α and β, which suggests a distinct role for each chain in the recognition of antigen–MHC complexes. Here, we have provided detailed information on CDR3 in paired TCRs expressed on human CD8+ T cells and established the basis of a reference set for αβ TCR repertoires in healthy humans. |
format | Online Article Text |
id | pubmed-6668380 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-66683802019-08-06 Comparative analysis of CDR3 regions in paired human αβ CD8 T cells Yu, Kun Shi, Ji Lu, Dan Yang, Qiong FEBS Open Bio Research Articles The majority of human CD8 cytotoxic T lymphocytes express αβ T‐cell receptors that recognize peptide–MHC class I complexes. Considerable attention has been devoted to TCR β repertoires, but study of TCR α chains has been limited. To gain a better understanding of the features of CDR3α and CDR3β in paired samples, we comprehensively analyzed 776 unique paired αβ TCR CDR3 regions in this study. We found that (I) the CDR3 length among paired αβ TCRs had a fairly narrow distribution due to random assortment of CDR3 length in alpha and beta chains; (II) nucleotide deletions among CDR3 regions were positively correlated with insertions in both α and β TCRs; (III) the CDR3 loops of both α and β chains contained an abundance of charged/polar residues and the CDR3 base regions contained a conserved motif; and (IV) the occurrence of Gly was CDR3 length‐ and position‐dependent in both chains, whereas the frequency of Ser at positions 106 and 107 was positively correlated with CDR3 length in TCR β. Overall, the amino acids in CDR3 loop regions were significantly different between TCR α and β, which suggests a distinct role for each chain in the recognition of antigen–MHC complexes. Here, we have provided detailed information on CDR3 in paired TCRs expressed on human CD8+ T cells and established the basis of a reference set for αβ TCR repertoires in healthy humans. John Wiley and Sons Inc. 2019-07-12 /pmc/articles/PMC6668380/ /pubmed/31237075 http://dx.doi.org/10.1002/2211-5463.12690 Text en © 2019 The Authors. Published by FEBS Press and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Yu, Kun Shi, Ji Lu, Dan Yang, Qiong Comparative analysis of CDR3 regions in paired human αβ CD8 T cells |
title | Comparative analysis of CDR3 regions in paired human αβ CD8 T cells |
title_full | Comparative analysis of CDR3 regions in paired human αβ CD8 T cells |
title_fullStr | Comparative analysis of CDR3 regions in paired human αβ CD8 T cells |
title_full_unstemmed | Comparative analysis of CDR3 regions in paired human αβ CD8 T cells |
title_short | Comparative analysis of CDR3 regions in paired human αβ CD8 T cells |
title_sort | comparative analysis of cdr3 regions in paired human αβ cd8 t cells |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6668380/ https://www.ncbi.nlm.nih.gov/pubmed/31237075 http://dx.doi.org/10.1002/2211-5463.12690 |
work_keys_str_mv | AT yukun comparativeanalysisofcdr3regionsinpairedhumanabcd8tcells AT shiji comparativeanalysisofcdr3regionsinpairedhumanabcd8tcells AT ludan comparativeanalysisofcdr3regionsinpairedhumanabcd8tcells AT yangqiong comparativeanalysisofcdr3regionsinpairedhumanabcd8tcells |