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Site-Specific DC Surface Signatures Influence CD4(+) T Cell Co-stimulation and Lung-Homing
Dendritic cells (DCs) that drain the gut and skin are known to favor the establishment of T cell populations that home to the original site of DC-antigen (Ag) encounter by providing soluble “imprinting” signals to T cells in the lymph node (LN). To study the induction of lung T cell-trafficking, we...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6668556/ https://www.ncbi.nlm.nih.gov/pubmed/31396211 http://dx.doi.org/10.3389/fimmu.2019.01650 |
Sumario: | Dendritic cells (DCs) that drain the gut and skin are known to favor the establishment of T cell populations that home to the original site of DC-antigen (Ag) encounter by providing soluble “imprinting” signals to T cells in the lymph node (LN). To study the induction of lung T cell-trafficking, we used a protein-adjuvant murine intranasal and intramuscular immunization model to compare in vivo-activated Ag(+) DCs in the lung and muscle-draining LNs. Higher frequencies of Ag(+) CD11b(+) DCs were observed in lung-draining mediastinal LNs (MedLN) compared to muscle-draining inguinal LNs (ILN). Ag(+) CD11b(+) MedLN DCs were qualitatively superior at priming CD4(+) T cells, which then expressed CD49a and CXCR3, and preferentially trafficked into the lung parenchyma. CD11b(+) DCs from the MedLN expressed higher levels of surface podoplanin, Trem4, GL7, and the known co-stimulatory molecules CD80, CD86, and CD24. Blockade of specific MedLN DC molecules or the use of sorted DC and T cell co-cultures demonstrated that DC surface phenotype influences the ability to prime T cells that then home to the lung. Thus, the density of dLN Ag(+) DCs, and DC surface molecule signatures are factors that can influence the output and differentiation of lung-homing CD4(+) T cells. |
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