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Anti-glycan IgM repertoires in newborn human cord blood

Natural antibodies are an innate-like subset of serum antibodies involved in host defense, tumor surveillance, homeostasis, and autoimmunity. Defining the natural antibody repertoire is critical for identifying biomarkers, developing vaccines, controlling and preventing autoimmunity, and understandi...

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Autores principales: Xia, Li, Gildersleeve, Jeffrey C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6668783/
https://www.ncbi.nlm.nih.gov/pubmed/31365539
http://dx.doi.org/10.1371/journal.pone.0218575
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author Xia, Li
Gildersleeve, Jeffrey C.
author_facet Xia, Li
Gildersleeve, Jeffrey C.
author_sort Xia, Li
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description Natural antibodies are an innate-like subset of serum antibodies involved in host defense, tumor surveillance, homeostasis, and autoimmunity. Defining the natural antibody repertoire is critical for identifying biomarkers, developing vaccines, controlling and preventing autoimmunity, and understanding the development and organization of the immune system. While natural antibodies to protein antigens have been studied in depth, little is known about natural antibodies to carbohydrate antigens. To address this, we profiled IgM from umbilical cord blood and matched maternal sera on a glycan microarray. Since standard methods to detect maternal contamination in cord serum did not have sufficient sensitivity for our study, we developed a highly sensitive microarray-based assay. Using this method, we found that over 50% of the cord samples had unacceptable levels of maternal contamination. For the cord samples with high purity, anti-glycan IgM antibodies were prevalent and recognized a broad range of non-human and human glycans. Using principal component analysis and hierarchical clustering, cord IgM repertoires showed a high degree of similarity with each other but were distinct from maternal IgM repertoires. Our results demonstrate that many anti-glycan antibodies in human serum are natural antibodies and provide new insights into the development of anti-glycan antibody repertoires.
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spelling pubmed-66687832019-08-06 Anti-glycan IgM repertoires in newborn human cord blood Xia, Li Gildersleeve, Jeffrey C. PLoS One Research Article Natural antibodies are an innate-like subset of serum antibodies involved in host defense, tumor surveillance, homeostasis, and autoimmunity. Defining the natural antibody repertoire is critical for identifying biomarkers, developing vaccines, controlling and preventing autoimmunity, and understanding the development and organization of the immune system. While natural antibodies to protein antigens have been studied in depth, little is known about natural antibodies to carbohydrate antigens. To address this, we profiled IgM from umbilical cord blood and matched maternal sera on a glycan microarray. Since standard methods to detect maternal contamination in cord serum did not have sufficient sensitivity for our study, we developed a highly sensitive microarray-based assay. Using this method, we found that over 50% of the cord samples had unacceptable levels of maternal contamination. For the cord samples with high purity, anti-glycan IgM antibodies were prevalent and recognized a broad range of non-human and human glycans. Using principal component analysis and hierarchical clustering, cord IgM repertoires showed a high degree of similarity with each other but were distinct from maternal IgM repertoires. Our results demonstrate that many anti-glycan antibodies in human serum are natural antibodies and provide new insights into the development of anti-glycan antibody repertoires. Public Library of Science 2019-07-31 /pmc/articles/PMC6668783/ /pubmed/31365539 http://dx.doi.org/10.1371/journal.pone.0218575 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Xia, Li
Gildersleeve, Jeffrey C.
Anti-glycan IgM repertoires in newborn human cord blood
title Anti-glycan IgM repertoires in newborn human cord blood
title_full Anti-glycan IgM repertoires in newborn human cord blood
title_fullStr Anti-glycan IgM repertoires in newborn human cord blood
title_full_unstemmed Anti-glycan IgM repertoires in newborn human cord blood
title_short Anti-glycan IgM repertoires in newborn human cord blood
title_sort anti-glycan igm repertoires in newborn human cord blood
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6668783/
https://www.ncbi.nlm.nih.gov/pubmed/31365539
http://dx.doi.org/10.1371/journal.pone.0218575
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