Cargando…

No Evidence for Effect of Exercise on Transcriptome of NK Cells in Breast Cancer Patients Undergoing Adjuvant Therapy: Results From a Pilot Study

BACKGROUND: Mobilization and activation of natural killer cells (NK cells) have been hypothesized to contribute to observed protective effects of exercise on cancer development and progression. Some evidence exists for acute effects of aerobic exercise on NK cell mobilization and function, i.e., alt...

Descripción completa

Detalles Bibliográficos
Autores principales: Pal, Anasua, Zimmer, Philipp, Schmidt, Martina E., Hummel, Manuela, Ulrich, Cornelia M., Wiskemann, Joachim, Steindorf, Karen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6669353/
https://www.ncbi.nlm.nih.gov/pubmed/31402874
http://dx.doi.org/10.3389/fphys.2019.00959
_version_ 1783440355291561984
author Pal, Anasua
Zimmer, Philipp
Schmidt, Martina E.
Hummel, Manuela
Ulrich, Cornelia M.
Wiskemann, Joachim
Steindorf, Karen
author_facet Pal, Anasua
Zimmer, Philipp
Schmidt, Martina E.
Hummel, Manuela
Ulrich, Cornelia M.
Wiskemann, Joachim
Steindorf, Karen
author_sort Pal, Anasua
collection PubMed
description BACKGROUND: Mobilization and activation of natural killer cells (NK cells) have been hypothesized to contribute to observed protective effects of exercise on cancer development and progression. Some evidence exists for acute effects of aerobic exercise on NK cell mobilization and function, i.e., alteration of the gene expression profile of NK cells. Yet, the chronic effects of exercise training, and effects of other modalities than endurance exercise are still understudied. Here, we investigated the chronic effects of a 12-week resistance exercise program on NK cell gene expression in breast cancer patients undergoing adjuvant chemo- or radiotherapy. METHODS: Breast cancer patients were randomly assigned to either a 12-week resistance exercise program or a relaxation control group concomitant to adjuvant therapy. In a subsample of 19 participants, RNA was extracted from magnet bead isolated NK cells and subsequently analyzed for differential gene expression using microarray Illumina HumanHT-12 v4 before and after the intervention. RESULTS: After chronic exercise intervention several genes showed higher differential expression compared to the control group. However, after correction for multiple testing, baseline-adjusted analyses of covariance indicated no significant differences between the intervention and the control group with regard to the gene expression profile. DISCUSSION: Our findings suggest that 12-week resistance-exercise did not alter the gene expression profile of NK cells in breast cancer patients undergoing adjuvant therapy on the long term. Further studies with larger sample sizes and specifically designed to investigate whether exercise-induced changes in NK cell function are attributed to acute effects are warranted.
format Online
Article
Text
id pubmed-6669353
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-66693532019-08-09 No Evidence for Effect of Exercise on Transcriptome of NK Cells in Breast Cancer Patients Undergoing Adjuvant Therapy: Results From a Pilot Study Pal, Anasua Zimmer, Philipp Schmidt, Martina E. Hummel, Manuela Ulrich, Cornelia M. Wiskemann, Joachim Steindorf, Karen Front Physiol Physiology BACKGROUND: Mobilization and activation of natural killer cells (NK cells) have been hypothesized to contribute to observed protective effects of exercise on cancer development and progression. Some evidence exists for acute effects of aerobic exercise on NK cell mobilization and function, i.e., alteration of the gene expression profile of NK cells. Yet, the chronic effects of exercise training, and effects of other modalities than endurance exercise are still understudied. Here, we investigated the chronic effects of a 12-week resistance exercise program on NK cell gene expression in breast cancer patients undergoing adjuvant chemo- or radiotherapy. METHODS: Breast cancer patients were randomly assigned to either a 12-week resistance exercise program or a relaxation control group concomitant to adjuvant therapy. In a subsample of 19 participants, RNA was extracted from magnet bead isolated NK cells and subsequently analyzed for differential gene expression using microarray Illumina HumanHT-12 v4 before and after the intervention. RESULTS: After chronic exercise intervention several genes showed higher differential expression compared to the control group. However, after correction for multiple testing, baseline-adjusted analyses of covariance indicated no significant differences between the intervention and the control group with regard to the gene expression profile. DISCUSSION: Our findings suggest that 12-week resistance-exercise did not alter the gene expression profile of NK cells in breast cancer patients undergoing adjuvant therapy on the long term. Further studies with larger sample sizes and specifically designed to investigate whether exercise-induced changes in NK cell function are attributed to acute effects are warranted. Frontiers Media S.A. 2019-07-25 /pmc/articles/PMC6669353/ /pubmed/31402874 http://dx.doi.org/10.3389/fphys.2019.00959 Text en Copyright © 2019 Pal, Zimmer, Schmidt, Hummel, Ulrich, Wiskemann and Steindorf. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Pal, Anasua
Zimmer, Philipp
Schmidt, Martina E.
Hummel, Manuela
Ulrich, Cornelia M.
Wiskemann, Joachim
Steindorf, Karen
No Evidence for Effect of Exercise on Transcriptome of NK Cells in Breast Cancer Patients Undergoing Adjuvant Therapy: Results From a Pilot Study
title No Evidence for Effect of Exercise on Transcriptome of NK Cells in Breast Cancer Patients Undergoing Adjuvant Therapy: Results From a Pilot Study
title_full No Evidence for Effect of Exercise on Transcriptome of NK Cells in Breast Cancer Patients Undergoing Adjuvant Therapy: Results From a Pilot Study
title_fullStr No Evidence for Effect of Exercise on Transcriptome of NK Cells in Breast Cancer Patients Undergoing Adjuvant Therapy: Results From a Pilot Study
title_full_unstemmed No Evidence for Effect of Exercise on Transcriptome of NK Cells in Breast Cancer Patients Undergoing Adjuvant Therapy: Results From a Pilot Study
title_short No Evidence for Effect of Exercise on Transcriptome of NK Cells in Breast Cancer Patients Undergoing Adjuvant Therapy: Results From a Pilot Study
title_sort no evidence for effect of exercise on transcriptome of nk cells in breast cancer patients undergoing adjuvant therapy: results from a pilot study
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6669353/
https://www.ncbi.nlm.nih.gov/pubmed/31402874
http://dx.doi.org/10.3389/fphys.2019.00959
work_keys_str_mv AT palanasua noevidenceforeffectofexerciseontranscriptomeofnkcellsinbreastcancerpatientsundergoingadjuvanttherapyresultsfromapilotstudy
AT zimmerphilipp noevidenceforeffectofexerciseontranscriptomeofnkcellsinbreastcancerpatientsundergoingadjuvanttherapyresultsfromapilotstudy
AT schmidtmartinae noevidenceforeffectofexerciseontranscriptomeofnkcellsinbreastcancerpatientsundergoingadjuvanttherapyresultsfromapilotstudy
AT hummelmanuela noevidenceforeffectofexerciseontranscriptomeofnkcellsinbreastcancerpatientsundergoingadjuvanttherapyresultsfromapilotstudy
AT ulrichcorneliam noevidenceforeffectofexerciseontranscriptomeofnkcellsinbreastcancerpatientsundergoingadjuvanttherapyresultsfromapilotstudy
AT wiskemannjoachim noevidenceforeffectofexerciseontranscriptomeofnkcellsinbreastcancerpatientsundergoingadjuvanttherapyresultsfromapilotstudy
AT steindorfkaren noevidenceforeffectofexerciseontranscriptomeofnkcellsinbreastcancerpatientsundergoingadjuvanttherapyresultsfromapilotstudy