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Haemostatic Changes in Five Patients Infected with Ebola Virus

Knowledge on haemostatic changes in humans infected with Ebola virus is limited due to safety concerns and access to patient samples. Ethical approval was obtained to collect plasma samples from patients in Sierra Leone infected with Ebola virus over time and samples were analysed for clotting time,...

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Autores principales: Smither, Sophie J., O’Brien, Lyn M., Eastaugh, Lin, Woolley, Tom, Lever, Mark, Fletcher, Tom, Parmar, Kiran, Hunt, Beverley J., Watts, Sarah, Kirkman, Emrys
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6669445/
https://www.ncbi.nlm.nih.gov/pubmed/31311112
http://dx.doi.org/10.3390/v11070647
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author Smither, Sophie J.
O’Brien, Lyn M.
Eastaugh, Lin
Woolley, Tom
Lever, Mark
Fletcher, Tom
Parmar, Kiran
Hunt, Beverley J.
Watts, Sarah
Kirkman, Emrys
author_facet Smither, Sophie J.
O’Brien, Lyn M.
Eastaugh, Lin
Woolley, Tom
Lever, Mark
Fletcher, Tom
Parmar, Kiran
Hunt, Beverley J.
Watts, Sarah
Kirkman, Emrys
author_sort Smither, Sophie J.
collection PubMed
description Knowledge on haemostatic changes in humans infected with Ebola virus is limited due to safety concerns and access to patient samples. Ethical approval was obtained to collect plasma samples from patients in Sierra Leone infected with Ebola virus over time and samples were analysed for clotting time, fibrinogen, and D-dimer levels. Plasma from healthy volunteers was also collected by two methods to determine effect of centrifugation on test results as blood collected in Sierra Leone was not centrifuged. Collecting plasma without centrifugation only affected D-dimer values. Patients with Ebola virus disease had higher PT and APTT and D-dimer values than healthy humans with plasma collected in the same manner. Fibrinogen levels in patients with Ebola virus disease were normal or lower than values measured in healthy people. Clotting times and D-dimer levels were elevated during infection with Ebola virus but return to normal over time in patients that survived and therefore could be considered prognostic. Informative data can be obtained from plasma collected without centrifugation which could improve patient monitoring in hazardous environments.
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spelling pubmed-66694452019-08-08 Haemostatic Changes in Five Patients Infected with Ebola Virus Smither, Sophie J. O’Brien, Lyn M. Eastaugh, Lin Woolley, Tom Lever, Mark Fletcher, Tom Parmar, Kiran Hunt, Beverley J. Watts, Sarah Kirkman, Emrys Viruses Article Knowledge on haemostatic changes in humans infected with Ebola virus is limited due to safety concerns and access to patient samples. Ethical approval was obtained to collect plasma samples from patients in Sierra Leone infected with Ebola virus over time and samples were analysed for clotting time, fibrinogen, and D-dimer levels. Plasma from healthy volunteers was also collected by two methods to determine effect of centrifugation on test results as blood collected in Sierra Leone was not centrifuged. Collecting plasma without centrifugation only affected D-dimer values. Patients with Ebola virus disease had higher PT and APTT and D-dimer values than healthy humans with plasma collected in the same manner. Fibrinogen levels in patients with Ebola virus disease were normal or lower than values measured in healthy people. Clotting times and D-dimer levels were elevated during infection with Ebola virus but return to normal over time in patients that survived and therefore could be considered prognostic. Informative data can be obtained from plasma collected without centrifugation which could improve patient monitoring in hazardous environments. MDPI 2019-07-15 /pmc/articles/PMC6669445/ /pubmed/31311112 http://dx.doi.org/10.3390/v11070647 Text en © Crown copyright (2019), Dstl. This material is licensed under the terms of the Open Government Licence except where otherwise stated. To view this licence, visit http://www.nationalarchives.gov.uk/doc/open-government-licence/version/3 or write to the Information Policy Team, The National Archives, Kew, London TW9 4DU, or email: psi@nationalarchives.gov.uk.
spellingShingle Article
Smither, Sophie J.
O’Brien, Lyn M.
Eastaugh, Lin
Woolley, Tom
Lever, Mark
Fletcher, Tom
Parmar, Kiran
Hunt, Beverley J.
Watts, Sarah
Kirkman, Emrys
Haemostatic Changes in Five Patients Infected with Ebola Virus
title Haemostatic Changes in Five Patients Infected with Ebola Virus
title_full Haemostatic Changes in Five Patients Infected with Ebola Virus
title_fullStr Haemostatic Changes in Five Patients Infected with Ebola Virus
title_full_unstemmed Haemostatic Changes in Five Patients Infected with Ebola Virus
title_short Haemostatic Changes in Five Patients Infected with Ebola Virus
title_sort haemostatic changes in five patients infected with ebola virus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6669445/
https://www.ncbi.nlm.nih.gov/pubmed/31311112
http://dx.doi.org/10.3390/v11070647
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