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Ranaviruses Bind Cells from Different Species through Interaction with Heparan Sulfate

Ranavirus cross-species infections have been documented, but the viral proteins involved in the interaction with cell receptors have not yet been identified. Here, viral cell-binding proteins and their cognate cellular receptors were investigated using two ranaviruses, Andrias davidianus ranavirus (...

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Autores principales: Ke, Fei, Wang, Zi-Hao, Ming, Cheng-Yue, Zhang, Qi-Ya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6669447/
https://www.ncbi.nlm.nih.gov/pubmed/31261956
http://dx.doi.org/10.3390/v11070593
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author Ke, Fei
Wang, Zi-Hao
Ming, Cheng-Yue
Zhang, Qi-Ya
author_facet Ke, Fei
Wang, Zi-Hao
Ming, Cheng-Yue
Zhang, Qi-Ya
author_sort Ke, Fei
collection PubMed
description Ranavirus cross-species infections have been documented, but the viral proteins involved in the interaction with cell receptors have not yet been identified. Here, viral cell-binding proteins and their cognate cellular receptors were investigated using two ranaviruses, Andrias davidianus ranavirus (ADRV) and Rana grylio virus (RGV), and two different cell lines, Chinese giant salamander thymus cells (GSTC) and Epithelioma papulosum cyprinid (EPC) cells. The heparan sulfate (HS) analog heparin inhibited plaque formation of ADRV and RGV in the two cell lines by more than 80% at a concentration of 5 μg/mL. In addition, enzymatic removal of cell surface HS by heparinase I markedly reduced plaque formation by both viruses and competition with heparin reduced virus-cell binding. These results indicate that cell surface HS is involved in ADRV and RGV cell binding and infection. Furthermore, recombinant viral envelope proteins ADRV-58L and RGV-53R bound heparin-Sepharose beads implying the potential that cell surface HS is involved in the initial interaction between ranaviruses and susceptible host cells. To our knowledge, this is the first report identifying cell surface HS as ranavirus binding factor and furthers understanding of interactions between ranaviruses and host cells.
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spelling pubmed-66694472019-08-08 Ranaviruses Bind Cells from Different Species through Interaction with Heparan Sulfate Ke, Fei Wang, Zi-Hao Ming, Cheng-Yue Zhang, Qi-Ya Viruses Article Ranavirus cross-species infections have been documented, but the viral proteins involved in the interaction with cell receptors have not yet been identified. Here, viral cell-binding proteins and their cognate cellular receptors were investigated using two ranaviruses, Andrias davidianus ranavirus (ADRV) and Rana grylio virus (RGV), and two different cell lines, Chinese giant salamander thymus cells (GSTC) and Epithelioma papulosum cyprinid (EPC) cells. The heparan sulfate (HS) analog heparin inhibited plaque formation of ADRV and RGV in the two cell lines by more than 80% at a concentration of 5 μg/mL. In addition, enzymatic removal of cell surface HS by heparinase I markedly reduced plaque formation by both viruses and competition with heparin reduced virus-cell binding. These results indicate that cell surface HS is involved in ADRV and RGV cell binding and infection. Furthermore, recombinant viral envelope proteins ADRV-58L and RGV-53R bound heparin-Sepharose beads implying the potential that cell surface HS is involved in the initial interaction between ranaviruses and susceptible host cells. To our knowledge, this is the first report identifying cell surface HS as ranavirus binding factor and furthers understanding of interactions between ranaviruses and host cells. MDPI 2019-06-29 /pmc/articles/PMC6669447/ /pubmed/31261956 http://dx.doi.org/10.3390/v11070593 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ke, Fei
Wang, Zi-Hao
Ming, Cheng-Yue
Zhang, Qi-Ya
Ranaviruses Bind Cells from Different Species through Interaction with Heparan Sulfate
title Ranaviruses Bind Cells from Different Species through Interaction with Heparan Sulfate
title_full Ranaviruses Bind Cells from Different Species through Interaction with Heparan Sulfate
title_fullStr Ranaviruses Bind Cells from Different Species through Interaction with Heparan Sulfate
title_full_unstemmed Ranaviruses Bind Cells from Different Species through Interaction with Heparan Sulfate
title_short Ranaviruses Bind Cells from Different Species through Interaction with Heparan Sulfate
title_sort ranaviruses bind cells from different species through interaction with heparan sulfate
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6669447/
https://www.ncbi.nlm.nih.gov/pubmed/31261956
http://dx.doi.org/10.3390/v11070593
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