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Abalone Collagen Extracts Potentiate Stem Cell Properties of Human Epidermal Keratinocytes
Stem cell activities in human tissues are critical for tissue integrity and function. Maintaining keratinocyte stem cells (KSCs) stemness helps sustain healthy skin by supporting keratinocyte renewal, involving the formation of epidermal barriers. In this study, abalone collagen (AC) extracts with m...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6669461/ https://www.ncbi.nlm.nih.gov/pubmed/31330853 http://dx.doi.org/10.3390/md17070424 |
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author | Thaweekitphathanaphakdee, Sajee Chanvorachote, Pithi Prateepchinda, Sagaw Khongkow, Mattaka Sucontphunt, Apirada |
author_facet | Thaweekitphathanaphakdee, Sajee Chanvorachote, Pithi Prateepchinda, Sagaw Khongkow, Mattaka Sucontphunt, Apirada |
author_sort | Thaweekitphathanaphakdee, Sajee |
collection | PubMed |
description | Stem cell activities in human tissues are critical for tissue integrity and function. Maintaining keratinocyte stem cells (KSCs) stemness helps sustain healthy skin by supporting keratinocyte renewal, involving the formation of epidermal barriers. In this study, abalone collagen (AC) extracts with molecular weights of 3 kDa (AC 1) and 300 kDa (AC 2) were compared to the epidermal growth factor (EGF) for their effects on cell proliferation, cell migration (wound healing), spheroid formation, and the expression level of stem cell markers on human keratinocytes (HaCaT cells). Cell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and cell proliferation was quantified by ATP and DNA content analysis and Sulforhodamine B (SRB) assays. Cell migration assay was determined using the scratch wound healing test. Spheroid formation was evaluated and the expression level of stem cell markers was investigated by western blot analysis. The results showed that AC 1 at the concentration of 100 µg/mL could stimulate HaCaT cell proliferation, migration, spheroid formation, and the expression level of stem cell markers (keratin 19, β-catenin, ALDH1A1) compared to the control. In conclusion, a smaller molecular weight of abalone collagen extract exhibits a better effect on keratinocytes proliferation, migration, and stemness, which could be a potential active ingredient in cosmeceutical products. |
format | Online Article Text |
id | pubmed-6669461 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-66694612019-08-08 Abalone Collagen Extracts Potentiate Stem Cell Properties of Human Epidermal Keratinocytes Thaweekitphathanaphakdee, Sajee Chanvorachote, Pithi Prateepchinda, Sagaw Khongkow, Mattaka Sucontphunt, Apirada Mar Drugs Article Stem cell activities in human tissues are critical for tissue integrity and function. Maintaining keratinocyte stem cells (KSCs) stemness helps sustain healthy skin by supporting keratinocyte renewal, involving the formation of epidermal barriers. In this study, abalone collagen (AC) extracts with molecular weights of 3 kDa (AC 1) and 300 kDa (AC 2) were compared to the epidermal growth factor (EGF) for their effects on cell proliferation, cell migration (wound healing), spheroid formation, and the expression level of stem cell markers on human keratinocytes (HaCaT cells). Cell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and cell proliferation was quantified by ATP and DNA content analysis and Sulforhodamine B (SRB) assays. Cell migration assay was determined using the scratch wound healing test. Spheroid formation was evaluated and the expression level of stem cell markers was investigated by western blot analysis. The results showed that AC 1 at the concentration of 100 µg/mL could stimulate HaCaT cell proliferation, migration, spheroid formation, and the expression level of stem cell markers (keratin 19, β-catenin, ALDH1A1) compared to the control. In conclusion, a smaller molecular weight of abalone collagen extract exhibits a better effect on keratinocytes proliferation, migration, and stemness, which could be a potential active ingredient in cosmeceutical products. MDPI 2019-07-20 /pmc/articles/PMC6669461/ /pubmed/31330853 http://dx.doi.org/10.3390/md17070424 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Thaweekitphathanaphakdee, Sajee Chanvorachote, Pithi Prateepchinda, Sagaw Khongkow, Mattaka Sucontphunt, Apirada Abalone Collagen Extracts Potentiate Stem Cell Properties of Human Epidermal Keratinocytes |
title | Abalone Collagen Extracts Potentiate Stem Cell Properties of Human Epidermal Keratinocytes |
title_full | Abalone Collagen Extracts Potentiate Stem Cell Properties of Human Epidermal Keratinocytes |
title_fullStr | Abalone Collagen Extracts Potentiate Stem Cell Properties of Human Epidermal Keratinocytes |
title_full_unstemmed | Abalone Collagen Extracts Potentiate Stem Cell Properties of Human Epidermal Keratinocytes |
title_short | Abalone Collagen Extracts Potentiate Stem Cell Properties of Human Epidermal Keratinocytes |
title_sort | abalone collagen extracts potentiate stem cell properties of human epidermal keratinocytes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6669461/ https://www.ncbi.nlm.nih.gov/pubmed/31330853 http://dx.doi.org/10.3390/md17070424 |
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