Cargando…
Identification, Pathogenicity of Novel Fowl Adenovirus Serotype 4 SDJN0105 in Shandong, China and Immunoprotective Evaluation of the Newly Developed Inactivated Oil-emulsion FAdV-4 Vaccine
Since mid-2015, numerous outbreaks of hydropericardium-hepatitis syndrome (HHS), which is caused by a novel fowl adenovirus serotype 4 (FAdV-4), have been reported in chickens in parts of China, thereby causing huge economic losses to the poultry industry. Thus, an effective vaccine to control the f...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6669483/ https://www.ncbi.nlm.nih.gov/pubmed/31288442 http://dx.doi.org/10.3390/v11070627 |
_version_ | 1783440380954411008 |
---|---|
author | Meng, Kai Yuan, Xiaoyuan Yu, Jiang Zhang, Yuxia Ai, Wu Wang, Youling |
author_facet | Meng, Kai Yuan, Xiaoyuan Yu, Jiang Zhang, Yuxia Ai, Wu Wang, Youling |
author_sort | Meng, Kai |
collection | PubMed |
description | Since mid-2015, numerous outbreaks of hydropericardium-hepatitis syndrome (HHS), which is caused by a novel fowl adenovirus serotype 4 (FAdV-4), have been reported in chickens in parts of China, thereby causing huge economic losses to the poultry industry. Thus, an effective vaccine to control the further spread of infections with this hyper-virulent FAdV-4 is imperative. In this study, we isolated a novel FAdV-4 strain SDJN0105 from a broiler farm with HHS disease in Shandong Province. Pathogenicity was evaluated by the observation of clinical symptoms, necropsy changes, and pathological tissue sections after oral and intramuscular (IM) infection of Specific pathogen free (SPF) chickens. The chickens infected by IM injection all died within three days, and chickens infected via the oculonasal route died within five days post-infection (dpi). Histopathological examination revealed that the pathology was confined to heart, liver, spleen, lung, kidney, and particularly the liver. Irrespective of the inoculation route, the highest viral DNA copy numbers were detected in the livers of infected chickens. The mRNA expression levels of IL-1β, IL-6, IL-8, IFNs, TNF-α, Mx, and OASL were significantly upregulated during the viral infection. In addition, an inactivated oil-emulsion FAdV-4 vaccine was developed. The vaccine could provide full protection for SPF chickens against a lethal dose of the FAdV-4 strain SDJN0105 and a high level of antibodies. These results improve our understanding of the innate immune responses in chickens infected with FAdV-4 and the pathogenesis of FAdV-4 caused by host factors, and the developed FAdV-4 vaccine is promising as a drug candidate for the prevention and reduction of the spread of HHS in poultry in China. |
format | Online Article Text |
id | pubmed-6669483 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-66694832019-08-08 Identification, Pathogenicity of Novel Fowl Adenovirus Serotype 4 SDJN0105 in Shandong, China and Immunoprotective Evaluation of the Newly Developed Inactivated Oil-emulsion FAdV-4 Vaccine Meng, Kai Yuan, Xiaoyuan Yu, Jiang Zhang, Yuxia Ai, Wu Wang, Youling Viruses Article Since mid-2015, numerous outbreaks of hydropericardium-hepatitis syndrome (HHS), which is caused by a novel fowl adenovirus serotype 4 (FAdV-4), have been reported in chickens in parts of China, thereby causing huge economic losses to the poultry industry. Thus, an effective vaccine to control the further spread of infections with this hyper-virulent FAdV-4 is imperative. In this study, we isolated a novel FAdV-4 strain SDJN0105 from a broiler farm with HHS disease in Shandong Province. Pathogenicity was evaluated by the observation of clinical symptoms, necropsy changes, and pathological tissue sections after oral and intramuscular (IM) infection of Specific pathogen free (SPF) chickens. The chickens infected by IM injection all died within three days, and chickens infected via the oculonasal route died within five days post-infection (dpi). Histopathological examination revealed that the pathology was confined to heart, liver, spleen, lung, kidney, and particularly the liver. Irrespective of the inoculation route, the highest viral DNA copy numbers were detected in the livers of infected chickens. The mRNA expression levels of IL-1β, IL-6, IL-8, IFNs, TNF-α, Mx, and OASL were significantly upregulated during the viral infection. In addition, an inactivated oil-emulsion FAdV-4 vaccine was developed. The vaccine could provide full protection for SPF chickens against a lethal dose of the FAdV-4 strain SDJN0105 and a high level of antibodies. These results improve our understanding of the innate immune responses in chickens infected with FAdV-4 and the pathogenesis of FAdV-4 caused by host factors, and the developed FAdV-4 vaccine is promising as a drug candidate for the prevention and reduction of the spread of HHS in poultry in China. MDPI 2019-07-08 /pmc/articles/PMC6669483/ /pubmed/31288442 http://dx.doi.org/10.3390/v11070627 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Meng, Kai Yuan, Xiaoyuan Yu, Jiang Zhang, Yuxia Ai, Wu Wang, Youling Identification, Pathogenicity of Novel Fowl Adenovirus Serotype 4 SDJN0105 in Shandong, China and Immunoprotective Evaluation of the Newly Developed Inactivated Oil-emulsion FAdV-4 Vaccine |
title | Identification, Pathogenicity of Novel Fowl Adenovirus Serotype 4 SDJN0105 in Shandong, China and Immunoprotective Evaluation of the Newly Developed Inactivated Oil-emulsion FAdV-4 Vaccine |
title_full | Identification, Pathogenicity of Novel Fowl Adenovirus Serotype 4 SDJN0105 in Shandong, China and Immunoprotective Evaluation of the Newly Developed Inactivated Oil-emulsion FAdV-4 Vaccine |
title_fullStr | Identification, Pathogenicity of Novel Fowl Adenovirus Serotype 4 SDJN0105 in Shandong, China and Immunoprotective Evaluation of the Newly Developed Inactivated Oil-emulsion FAdV-4 Vaccine |
title_full_unstemmed | Identification, Pathogenicity of Novel Fowl Adenovirus Serotype 4 SDJN0105 in Shandong, China and Immunoprotective Evaluation of the Newly Developed Inactivated Oil-emulsion FAdV-4 Vaccine |
title_short | Identification, Pathogenicity of Novel Fowl Adenovirus Serotype 4 SDJN0105 in Shandong, China and Immunoprotective Evaluation of the Newly Developed Inactivated Oil-emulsion FAdV-4 Vaccine |
title_sort | identification, pathogenicity of novel fowl adenovirus serotype 4 sdjn0105 in shandong, china and immunoprotective evaluation of the newly developed inactivated oil-emulsion fadv-4 vaccine |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6669483/ https://www.ncbi.nlm.nih.gov/pubmed/31288442 http://dx.doi.org/10.3390/v11070627 |
work_keys_str_mv | AT mengkai identificationpathogenicityofnovelfowladenovirusserotype4sdjn0105inshandongchinaandimmunoprotectiveevaluationofthenewlydevelopedinactivatedoilemulsionfadv4vaccine AT yuanxiaoyuan identificationpathogenicityofnovelfowladenovirusserotype4sdjn0105inshandongchinaandimmunoprotectiveevaluationofthenewlydevelopedinactivatedoilemulsionfadv4vaccine AT yujiang identificationpathogenicityofnovelfowladenovirusserotype4sdjn0105inshandongchinaandimmunoprotectiveevaluationofthenewlydevelopedinactivatedoilemulsionfadv4vaccine AT zhangyuxia identificationpathogenicityofnovelfowladenovirusserotype4sdjn0105inshandongchinaandimmunoprotectiveevaluationofthenewlydevelopedinactivatedoilemulsionfadv4vaccine AT aiwu identificationpathogenicityofnovelfowladenovirusserotype4sdjn0105inshandongchinaandimmunoprotectiveevaluationofthenewlydevelopedinactivatedoilemulsionfadv4vaccine AT wangyouling identificationpathogenicityofnovelfowladenovirusserotype4sdjn0105inshandongchinaandimmunoprotectiveevaluationofthenewlydevelopedinactivatedoilemulsionfadv4vaccine |