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The Damaging Effects of Pedunsaponin A on Pomacea canaliculata Hemocytes

Pomacea canaliculata hemocytes are the main functional cells in the immune defense system, and hemocyte destruction disrupts the immune response mechanism of P. canaliculata, resulting in abnormal growth, development, reproduction, and even death. Our previous study found that Pedunsaponin A signifi...

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Autores principales: Yang, Chunping, Lv, Tianxing, Wang, Bin, Qiu, Xiaoyan, Luo, Liya, Zhang, Min, Yue, Guizhou, Qin, Guangwei, Xie, Deshan, Chen, Huabao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6669518/
https://www.ncbi.nlm.nih.gov/pubmed/31277361
http://dx.doi.org/10.3390/toxins11070390
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author Yang, Chunping
Lv, Tianxing
Wang, Bin
Qiu, Xiaoyan
Luo, Liya
Zhang, Min
Yue, Guizhou
Qin, Guangwei
Xie, Deshan
Chen, Huabao
author_facet Yang, Chunping
Lv, Tianxing
Wang, Bin
Qiu, Xiaoyan
Luo, Liya
Zhang, Min
Yue, Guizhou
Qin, Guangwei
Xie, Deshan
Chen, Huabao
author_sort Yang, Chunping
collection PubMed
description Pomacea canaliculata hemocytes are the main functional cells in the immune defense system, and hemocyte destruction disrupts the immune response mechanism of P. canaliculata, resulting in abnormal growth, development, reproduction, and even death. Our previous study found that Pedunsaponin A significantly affects P. canaliculata hemocyte structure. This study further investigated the damaging effects of Pedunsaponin A on P. canaliculata hemocytes. The cell mortality rate results showed that the hemocyte mortality was significantly increased after treatment with Pedunsaponin A, and the mortality rate exhibited a significant positive correlation with treatment time and dose. The membrane potential results showed that the cell membranes of P. canaliculata hemocytes exhibited time-dependent membrane depolarization after 40 mg/L Pedunsaponin A treatment. At 36 h, the cell depolarization rate in the Pedunsaponin A treatment group was 41.43%, which was significantly greater than the control group (6.24%). The cytoskeleton results showed that Pedunsaponin A led to disordered and dispersed arrangement of microfilaments and changes in the cytoskeletal structure. The apoptosis and cell cycle results showed that Pedunsaponin A induced apoptosis and influenced the cell cycle to some extent. These results showed that the cell membrane and cytoskeleton of P. canaliculata hemocytes were damaged after treatment with Pedunsaponin A, which led to an increase in cell mortality, dysfunction, cell cycle abnormalities and apoptosis. This study provides a foundation for further identification of the site of Pedunsaponin A activity on hemocytes.
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spelling pubmed-66695182019-08-08 The Damaging Effects of Pedunsaponin A on Pomacea canaliculata Hemocytes Yang, Chunping Lv, Tianxing Wang, Bin Qiu, Xiaoyan Luo, Liya Zhang, Min Yue, Guizhou Qin, Guangwei Xie, Deshan Chen, Huabao Toxins (Basel) Article Pomacea canaliculata hemocytes are the main functional cells in the immune defense system, and hemocyte destruction disrupts the immune response mechanism of P. canaliculata, resulting in abnormal growth, development, reproduction, and even death. Our previous study found that Pedunsaponin A significantly affects P. canaliculata hemocyte structure. This study further investigated the damaging effects of Pedunsaponin A on P. canaliculata hemocytes. The cell mortality rate results showed that the hemocyte mortality was significantly increased after treatment with Pedunsaponin A, and the mortality rate exhibited a significant positive correlation with treatment time and dose. The membrane potential results showed that the cell membranes of P. canaliculata hemocytes exhibited time-dependent membrane depolarization after 40 mg/L Pedunsaponin A treatment. At 36 h, the cell depolarization rate in the Pedunsaponin A treatment group was 41.43%, which was significantly greater than the control group (6.24%). The cytoskeleton results showed that Pedunsaponin A led to disordered and dispersed arrangement of microfilaments and changes in the cytoskeletal structure. The apoptosis and cell cycle results showed that Pedunsaponin A induced apoptosis and influenced the cell cycle to some extent. These results showed that the cell membrane and cytoskeleton of P. canaliculata hemocytes were damaged after treatment with Pedunsaponin A, which led to an increase in cell mortality, dysfunction, cell cycle abnormalities and apoptosis. This study provides a foundation for further identification of the site of Pedunsaponin A activity on hemocytes. MDPI 2019-07-04 /pmc/articles/PMC6669518/ /pubmed/31277361 http://dx.doi.org/10.3390/toxins11070390 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yang, Chunping
Lv, Tianxing
Wang, Bin
Qiu, Xiaoyan
Luo, Liya
Zhang, Min
Yue, Guizhou
Qin, Guangwei
Xie, Deshan
Chen, Huabao
The Damaging Effects of Pedunsaponin A on Pomacea canaliculata Hemocytes
title The Damaging Effects of Pedunsaponin A on Pomacea canaliculata Hemocytes
title_full The Damaging Effects of Pedunsaponin A on Pomacea canaliculata Hemocytes
title_fullStr The Damaging Effects of Pedunsaponin A on Pomacea canaliculata Hemocytes
title_full_unstemmed The Damaging Effects of Pedunsaponin A on Pomacea canaliculata Hemocytes
title_short The Damaging Effects of Pedunsaponin A on Pomacea canaliculata Hemocytes
title_sort damaging effects of pedunsaponin a on pomacea canaliculata hemocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6669518/
https://www.ncbi.nlm.nih.gov/pubmed/31277361
http://dx.doi.org/10.3390/toxins11070390
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