Enzyme-Aided Extraction of Fucoidan by AMG Augments the Functionality of EPCs through Regulation of the AKT/Rheb Signaling Pathway

The purpose of the present study is to improve the endothelial progenitor cells (EPC) activation, proliferation, and angiogenesis using enzyme-aided extraction of fucoidan by amyloglucosidase (EAEF-AMG). Enzyme-aided extraction of fucoidan by AMG (EAEF-AMG) significantly increased EPC proliferation...

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Detalles Bibliográficos
Autores principales: Rethineswaran, Vinoth Kumar, Kim, Yeon-Ju, Jang, Woong Bi, Ji, Seung Taek, Kang, Songhwa, Kim, Da Yeon, Park, Ji Hye, Van, Le Thi Hong, Giang, Ly Thanh Truong, Ha, Jong Seong, Yun, Jisoo, Lee, Dong Hyung, Yu, Sun-Nyoung, Park, Sul-Gi, Ahn, Soon-Cheol, Kwon, Sang-Mo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6669526/
https://www.ncbi.nlm.nih.gov/pubmed/31277207
http://dx.doi.org/10.3390/md17070392
Descripción
Sumario:The purpose of the present study is to improve the endothelial progenitor cells (EPC) activation, proliferation, and angiogenesis using enzyme-aided extraction of fucoidan by amyloglucosidase (EAEF-AMG). Enzyme-aided extraction of fucoidan by AMG (EAEF-AMG) significantly increased EPC proliferation by reducing the reactive oxygen species (ROS) and decreasing apoptosis. Notably, EAEF-AMG treated EPCs repressed the colocalization of TSC2/LAMP1 and promoted perinuclear localization of mTOR/LAMP1 and mTOR/Rheb. Moreover, EAEF-AMG enhanced EPC functionalities, including tube formation, cell migration, and wound healing via regulation of AKT/Rheb signaling. Our data provided cell priming protocols to enhance therapeutic applications of EPCs using bioactive compounds for the treatment of CVD.

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