Enzyme-Aided Extraction of Fucoidan by AMG Augments the Functionality of EPCs through Regulation of the AKT/Rheb Signaling Pathway

The purpose of the present study is to improve the endothelial progenitor cells (EPC) activation, proliferation, and angiogenesis using enzyme-aided extraction of fucoidan by amyloglucosidase (EAEF-AMG). Enzyme-aided extraction of fucoidan by AMG (EAEF-AMG) significantly increased EPC proliferation...

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Autores principales: Rethineswaran, Vinoth Kumar, Kim, Yeon-Ju, Jang, Woong Bi, Ji, Seung Taek, Kang, Songhwa, Kim, Da Yeon, Park, Ji Hye, Van, Le Thi Hong, Giang, Ly Thanh Truong, Ha, Jong Seong, Yun, Jisoo, Lee, Dong Hyung, Yu, Sun-Nyoung, Park, Sul-Gi, Ahn, Soon-Cheol, Kwon, Sang-Mo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6669526/
https://www.ncbi.nlm.nih.gov/pubmed/31277207
http://dx.doi.org/10.3390/md17070392
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author Rethineswaran, Vinoth Kumar
Kim, Yeon-Ju
Jang, Woong Bi
Ji, Seung Taek
Kang, Songhwa
Kim, Da Yeon
Park, Ji Hye
Van, Le Thi Hong
Giang, Ly Thanh Truong
Ha, Jong Seong
Yun, Jisoo
Lee, Dong Hyung
Yu, Sun-Nyoung
Park, Sul-Gi
Ahn, Soon-Cheol
Kwon, Sang-Mo
author_facet Rethineswaran, Vinoth Kumar
Kim, Yeon-Ju
Jang, Woong Bi
Ji, Seung Taek
Kang, Songhwa
Kim, Da Yeon
Park, Ji Hye
Van, Le Thi Hong
Giang, Ly Thanh Truong
Ha, Jong Seong
Yun, Jisoo
Lee, Dong Hyung
Yu, Sun-Nyoung
Park, Sul-Gi
Ahn, Soon-Cheol
Kwon, Sang-Mo
author_sort Rethineswaran, Vinoth Kumar
collection PubMed
description The purpose of the present study is to improve the endothelial progenitor cells (EPC) activation, proliferation, and angiogenesis using enzyme-aided extraction of fucoidan by amyloglucosidase (EAEF-AMG). Enzyme-aided extraction of fucoidan by AMG (EAEF-AMG) significantly increased EPC proliferation by reducing the reactive oxygen species (ROS) and decreasing apoptosis. Notably, EAEF-AMG treated EPCs repressed the colocalization of TSC2/LAMP1 and promoted perinuclear localization of mTOR/LAMP1 and mTOR/Rheb. Moreover, EAEF-AMG enhanced EPC functionalities, including tube formation, cell migration, and wound healing via regulation of AKT/Rheb signaling. Our data provided cell priming protocols to enhance therapeutic applications of EPCs using bioactive compounds for the treatment of CVD.
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spelling pubmed-66695262019-08-08 Enzyme-Aided Extraction of Fucoidan by AMG Augments the Functionality of EPCs through Regulation of the AKT/Rheb Signaling Pathway Rethineswaran, Vinoth Kumar Kim, Yeon-Ju Jang, Woong Bi Ji, Seung Taek Kang, Songhwa Kim, Da Yeon Park, Ji Hye Van, Le Thi Hong Giang, Ly Thanh Truong Ha, Jong Seong Yun, Jisoo Lee, Dong Hyung Yu, Sun-Nyoung Park, Sul-Gi Ahn, Soon-Cheol Kwon, Sang-Mo Mar Drugs Article The purpose of the present study is to improve the endothelial progenitor cells (EPC) activation, proliferation, and angiogenesis using enzyme-aided extraction of fucoidan by amyloglucosidase (EAEF-AMG). Enzyme-aided extraction of fucoidan by AMG (EAEF-AMG) significantly increased EPC proliferation by reducing the reactive oxygen species (ROS) and decreasing apoptosis. Notably, EAEF-AMG treated EPCs repressed the colocalization of TSC2/LAMP1 and promoted perinuclear localization of mTOR/LAMP1 and mTOR/Rheb. Moreover, EAEF-AMG enhanced EPC functionalities, including tube formation, cell migration, and wound healing via regulation of AKT/Rheb signaling. Our data provided cell priming protocols to enhance therapeutic applications of EPCs using bioactive compounds for the treatment of CVD. MDPI 2019-07-03 /pmc/articles/PMC6669526/ /pubmed/31277207 http://dx.doi.org/10.3390/md17070392 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rethineswaran, Vinoth Kumar
Kim, Yeon-Ju
Jang, Woong Bi
Ji, Seung Taek
Kang, Songhwa
Kim, Da Yeon
Park, Ji Hye
Van, Le Thi Hong
Giang, Ly Thanh Truong
Ha, Jong Seong
Yun, Jisoo
Lee, Dong Hyung
Yu, Sun-Nyoung
Park, Sul-Gi
Ahn, Soon-Cheol
Kwon, Sang-Mo
Enzyme-Aided Extraction of Fucoidan by AMG Augments the Functionality of EPCs through Regulation of the AKT/Rheb Signaling Pathway
title Enzyme-Aided Extraction of Fucoidan by AMG Augments the Functionality of EPCs through Regulation of the AKT/Rheb Signaling Pathway
title_full Enzyme-Aided Extraction of Fucoidan by AMG Augments the Functionality of EPCs through Regulation of the AKT/Rheb Signaling Pathway
title_fullStr Enzyme-Aided Extraction of Fucoidan by AMG Augments the Functionality of EPCs through Regulation of the AKT/Rheb Signaling Pathway
title_full_unstemmed Enzyme-Aided Extraction of Fucoidan by AMG Augments the Functionality of EPCs through Regulation of the AKT/Rheb Signaling Pathway
title_short Enzyme-Aided Extraction of Fucoidan by AMG Augments the Functionality of EPCs through Regulation of the AKT/Rheb Signaling Pathway
title_sort enzyme-aided extraction of fucoidan by amg augments the functionality of epcs through regulation of the akt/rheb signaling pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6669526/
https://www.ncbi.nlm.nih.gov/pubmed/31277207
http://dx.doi.org/10.3390/md17070392
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