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Importance of the Host Phenotype on the Preservation of the Genetic Diversity in Codling Moth Granulovirus

To test the importance of the host genotype in maintaining virus genetic diversity, five experimental populations were constructed by mixing two Cydia pomonella granulovirus isolates, the Mexican isolate CpGV-M and the CpGV-R5, in ratios of 99% M + 1% R, 95% M + 5% R, 90% M + 10% R, 50% M + 50% R, a...

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Autores principales: Graillot, Benoit, Blachere-López, Christine, Besse, Samantha, Siegwart, Myriam, López-Ferber, Miguel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6669585/
https://www.ncbi.nlm.nih.gov/pubmed/31284495
http://dx.doi.org/10.3390/v11070621
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author Graillot, Benoit
Blachere-López, Christine
Besse, Samantha
Siegwart, Myriam
López-Ferber, Miguel
author_facet Graillot, Benoit
Blachere-López, Christine
Besse, Samantha
Siegwart, Myriam
López-Ferber, Miguel
author_sort Graillot, Benoit
collection PubMed
description To test the importance of the host genotype in maintaining virus genetic diversity, five experimental populations were constructed by mixing two Cydia pomonella granulovirus isolates, the Mexican isolate CpGV-M and the CpGV-R5, in ratios of 99% M + 1% R, 95% M + 5% R, 90% M + 10% R, 50% M + 50% R, and 10% M + 90% R. CpGV-M and CpGV-R5 differ in their ability to replicate in codling moth larvae carrying the type I resistance. This ability is associated with a genetic marker located in the virus pe38 gene. Six successive cycles of replication were carried out with each virus population on a fully-permissive codling moth colony (CpNPP), as well as on a host colony (R(GV)) that carries the type I resistance, and thus blocks CpGV-M replication. The infectivity of offspring viruses was tested on both hosts. Replication on the CpNPP leads to virus lineages preserving the pe38 markers characteristic of both isolates, while replication on the R(GV) colony drastically reduces the frequency of the CpGV-M pe38 marker. Virus progeny obtained after replication on CpNPP show consistently higher pathogenicity than that of progeny viruses obtained by replication on R(GV), independently of the host used for testing.
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spelling pubmed-66695852019-08-08 Importance of the Host Phenotype on the Preservation of the Genetic Diversity in Codling Moth Granulovirus Graillot, Benoit Blachere-López, Christine Besse, Samantha Siegwart, Myriam López-Ferber, Miguel Viruses Article To test the importance of the host genotype in maintaining virus genetic diversity, five experimental populations were constructed by mixing two Cydia pomonella granulovirus isolates, the Mexican isolate CpGV-M and the CpGV-R5, in ratios of 99% M + 1% R, 95% M + 5% R, 90% M + 10% R, 50% M + 50% R, and 10% M + 90% R. CpGV-M and CpGV-R5 differ in their ability to replicate in codling moth larvae carrying the type I resistance. This ability is associated with a genetic marker located in the virus pe38 gene. Six successive cycles of replication were carried out with each virus population on a fully-permissive codling moth colony (CpNPP), as well as on a host colony (R(GV)) that carries the type I resistance, and thus blocks CpGV-M replication. The infectivity of offspring viruses was tested on both hosts. Replication on the CpNPP leads to virus lineages preserving the pe38 markers characteristic of both isolates, while replication on the R(GV) colony drastically reduces the frequency of the CpGV-M pe38 marker. Virus progeny obtained after replication on CpNPP show consistently higher pathogenicity than that of progeny viruses obtained by replication on R(GV), independently of the host used for testing. MDPI 2019-07-05 /pmc/articles/PMC6669585/ /pubmed/31284495 http://dx.doi.org/10.3390/v11070621 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Graillot, Benoit
Blachere-López, Christine
Besse, Samantha
Siegwart, Myriam
López-Ferber, Miguel
Importance of the Host Phenotype on the Preservation of the Genetic Diversity in Codling Moth Granulovirus
title Importance of the Host Phenotype on the Preservation of the Genetic Diversity in Codling Moth Granulovirus
title_full Importance of the Host Phenotype on the Preservation of the Genetic Diversity in Codling Moth Granulovirus
title_fullStr Importance of the Host Phenotype on the Preservation of the Genetic Diversity in Codling Moth Granulovirus
title_full_unstemmed Importance of the Host Phenotype on the Preservation of the Genetic Diversity in Codling Moth Granulovirus
title_short Importance of the Host Phenotype on the Preservation of the Genetic Diversity in Codling Moth Granulovirus
title_sort importance of the host phenotype on the preservation of the genetic diversity in codling moth granulovirus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6669585/
https://www.ncbi.nlm.nih.gov/pubmed/31284495
http://dx.doi.org/10.3390/v11070621
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