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Low-Cost Localized Surface Plasmon Resonance Biosensing Platform with a Response Enhancement for Protein Detection

There are many potential applications for biosensors that can provide real-time analysis, such as environmental monitoring and disease prevention. In this study, we investigated a simple strategy for real-time protein detection, which had the advantages of affordability, fast response, portability,...

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Detalles Bibliográficos
Autores principales: Liu, Yun, Zhang, Ning, Li, Ping, Yu, Li, Chen, Shimeng, Zhang, Yang, Jing, Zhenguo, Peng, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6669697/
https://www.ncbi.nlm.nih.gov/pubmed/31315291
http://dx.doi.org/10.3390/nano9071019
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author Liu, Yun
Zhang, Ning
Li, Ping
Yu, Li
Chen, Shimeng
Zhang, Yang
Jing, Zhenguo
Peng, Wei
author_facet Liu, Yun
Zhang, Ning
Li, Ping
Yu, Li
Chen, Shimeng
Zhang, Yang
Jing, Zhenguo
Peng, Wei
author_sort Liu, Yun
collection PubMed
description There are many potential applications for biosensors that can provide real-time analysis, such as environmental monitoring and disease prevention. In this study, we investigated a simple strategy for real-time protein detection, which had the advantages of affordability, fast response, portability, and ease of use. A robust quantification of protein interaction was achieved by combining capillary localized surface plasmon resonance (LSPR) sensors and complementary metal–oxide–semiconductor (CMOS) image sensors. Gold nanoparticles were modified on the inner wall of the capillary, which was used as a microfluidic channel and sensing surface. We functionalized one of the LSPR sensors using ligand bound to gold nanoparticle. Our proposed biosensing platform could be easily multiplexed to achieve high throughput screening of biomolecular interactions, and it has the potential for use in disposable sensors. Moreover, the sensing signal was enhanced by the extinction effect of gold nanoparticles. The experimental results showed that our device could achieve qualitative identification and quantitative measurement of transferrin and immunoglobulin G (IgG). As a field-portable and low-cost optical platform, the proposed LSPR biosensing device is broadly applicable to various protein binding tests via a similar self-assembly of organic ultrathin films.
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spelling pubmed-66696972019-08-08 Low-Cost Localized Surface Plasmon Resonance Biosensing Platform with a Response Enhancement for Protein Detection Liu, Yun Zhang, Ning Li, Ping Yu, Li Chen, Shimeng Zhang, Yang Jing, Zhenguo Peng, Wei Nanomaterials (Basel) Article There are many potential applications for biosensors that can provide real-time analysis, such as environmental monitoring and disease prevention. In this study, we investigated a simple strategy for real-time protein detection, which had the advantages of affordability, fast response, portability, and ease of use. A robust quantification of protein interaction was achieved by combining capillary localized surface plasmon resonance (LSPR) sensors and complementary metal–oxide–semiconductor (CMOS) image sensors. Gold nanoparticles were modified on the inner wall of the capillary, which was used as a microfluidic channel and sensing surface. We functionalized one of the LSPR sensors using ligand bound to gold nanoparticle. Our proposed biosensing platform could be easily multiplexed to achieve high throughput screening of biomolecular interactions, and it has the potential for use in disposable sensors. Moreover, the sensing signal was enhanced by the extinction effect of gold nanoparticles. The experimental results showed that our device could achieve qualitative identification and quantitative measurement of transferrin and immunoglobulin G (IgG). As a field-portable and low-cost optical platform, the proposed LSPR biosensing device is broadly applicable to various protein binding tests via a similar self-assembly of organic ultrathin films. MDPI 2019-07-16 /pmc/articles/PMC6669697/ /pubmed/31315291 http://dx.doi.org/10.3390/nano9071019 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Yun
Zhang, Ning
Li, Ping
Yu, Li
Chen, Shimeng
Zhang, Yang
Jing, Zhenguo
Peng, Wei
Low-Cost Localized Surface Plasmon Resonance Biosensing Platform with a Response Enhancement for Protein Detection
title Low-Cost Localized Surface Plasmon Resonance Biosensing Platform with a Response Enhancement for Protein Detection
title_full Low-Cost Localized Surface Plasmon Resonance Biosensing Platform with a Response Enhancement for Protein Detection
title_fullStr Low-Cost Localized Surface Plasmon Resonance Biosensing Platform with a Response Enhancement for Protein Detection
title_full_unstemmed Low-Cost Localized Surface Plasmon Resonance Biosensing Platform with a Response Enhancement for Protein Detection
title_short Low-Cost Localized Surface Plasmon Resonance Biosensing Platform with a Response Enhancement for Protein Detection
title_sort low-cost localized surface plasmon resonance biosensing platform with a response enhancement for protein detection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6669697/
https://www.ncbi.nlm.nih.gov/pubmed/31315291
http://dx.doi.org/10.3390/nano9071019
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