Antiviral activity of Piscidin 1 against pseudorabies virus both in vitro and in vivo

BACKGROUND: Swine-origin virus infection spreading widely could cause significant economic loss to porcine industry. Novel antiviral agents need to be developed to control this situation. METHODS: In this study, we evaluated the activities of five broad-spectrum antimicrobial peptides (AMPs) against...

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Autores principales: Hu, Han, Guo, Nan, Chen, Shuhua, Guo, Xiaozhen, Liu, Xiaoli, Ye, Shiyi, Chai, Qingqing, Wang, Yang, Liu, Binlei, He, Qigai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6670175/
https://www.ncbi.nlm.nih.gov/pubmed/31366370
http://dx.doi.org/10.1186/s12985-019-1199-4
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author Hu, Han
Guo, Nan
Chen, Shuhua
Guo, Xiaozhen
Liu, Xiaoli
Ye, Shiyi
Chai, Qingqing
Wang, Yang
Liu, Binlei
He, Qigai
author_facet Hu, Han
Guo, Nan
Chen, Shuhua
Guo, Xiaozhen
Liu, Xiaoli
Ye, Shiyi
Chai, Qingqing
Wang, Yang
Liu, Binlei
He, Qigai
author_sort Hu, Han
collection PubMed
description BACKGROUND: Swine-origin virus infection spreading widely could cause significant economic loss to porcine industry. Novel antiviral agents need to be developed to control this situation. METHODS: In this study, we evaluated the activities of five broad-spectrum antimicrobial peptides (AMPs) against several important swine-origin pathogenic viruses by TCID(50) assay. Plaque reduction assay and cell apoptosis assay were also used to test the activity of the peptides. Protection effect of piscidin against pseudorabies virus (PRV) was also examined in mouse model. RESULTS: Piscidin (piscidin 1), caerin (caerin 1.1) and maculatin (maculatin 1.1) could inhibit PRV by direct interaction with the virus particles in a dose-dependent manner and they could also protect the cells from PRV-induced apoptosis. Among the peptides tested, piscidin showed the strongest activity against PRV. Moreover, in vivo assay showed that piscidin can reduce the mortality of mice infected with PRV. CONCLUSION: In vitro and in vivo experiments indicate that piscidin has antiviral activity against PRV.
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spelling pubmed-66701752019-08-06 Antiviral activity of Piscidin 1 against pseudorabies virus both in vitro and in vivo Hu, Han Guo, Nan Chen, Shuhua Guo, Xiaozhen Liu, Xiaoli Ye, Shiyi Chai, Qingqing Wang, Yang Liu, Binlei He, Qigai Virol J Research BACKGROUND: Swine-origin virus infection spreading widely could cause significant economic loss to porcine industry. Novel antiviral agents need to be developed to control this situation. METHODS: In this study, we evaluated the activities of five broad-spectrum antimicrobial peptides (AMPs) against several important swine-origin pathogenic viruses by TCID(50) assay. Plaque reduction assay and cell apoptosis assay were also used to test the activity of the peptides. Protection effect of piscidin against pseudorabies virus (PRV) was also examined in mouse model. RESULTS: Piscidin (piscidin 1), caerin (caerin 1.1) and maculatin (maculatin 1.1) could inhibit PRV by direct interaction with the virus particles in a dose-dependent manner and they could also protect the cells from PRV-induced apoptosis. Among the peptides tested, piscidin showed the strongest activity against PRV. Moreover, in vivo assay showed that piscidin can reduce the mortality of mice infected with PRV. CONCLUSION: In vitro and in vivo experiments indicate that piscidin has antiviral activity against PRV. BioMed Central 2019-07-31 /pmc/articles/PMC6670175/ /pubmed/31366370 http://dx.doi.org/10.1186/s12985-019-1199-4 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Hu, Han
Guo, Nan
Chen, Shuhua
Guo, Xiaozhen
Liu, Xiaoli
Ye, Shiyi
Chai, Qingqing
Wang, Yang
Liu, Binlei
He, Qigai
Antiviral activity of Piscidin 1 against pseudorabies virus both in vitro and in vivo
title Antiviral activity of Piscidin 1 against pseudorabies virus both in vitro and in vivo
title_full Antiviral activity of Piscidin 1 against pseudorabies virus both in vitro and in vivo
title_fullStr Antiviral activity of Piscidin 1 against pseudorabies virus both in vitro and in vivo
title_full_unstemmed Antiviral activity of Piscidin 1 against pseudorabies virus both in vitro and in vivo
title_short Antiviral activity of Piscidin 1 against pseudorabies virus both in vitro and in vivo
title_sort antiviral activity of piscidin 1 against pseudorabies virus both in vitro and in vivo
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6670175/
https://www.ncbi.nlm.nih.gov/pubmed/31366370
http://dx.doi.org/10.1186/s12985-019-1199-4
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