Highly efficient construction of infectious viroid-derived clones

BACKGROUND: Viroid research generally relies on infectious cDNA clones that consist of dimers of the entire viroid sequence. At present, those dimers are generated by self-ligation of monomeric cDNA, a strategy that presents several disadvantages: (i) low efficiency, (ii) it is a non-oriented reacti...

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Detalles Bibliográficos
Autores principales: Marquez-Molins, Joan, Navarro, Jose Antonio, Pallas, Vicente, Gomez, Gustavo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6670230/
https://www.ncbi.nlm.nih.gov/pubmed/31388344
http://dx.doi.org/10.1186/s13007-019-0470-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6670230/
https://www.ncbi.nlm.nih.gov/pubmed/31388344
http://dx.doi.org/10.1186/s13007-019-0470-4

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