Cargando…
Highly efficient construction of infectious viroid-derived clones
BACKGROUND: Viroid research generally relies on infectious cDNA clones that consist of dimers of the entire viroid sequence. At present, those dimers are generated by self-ligation of monomeric cDNA, a strategy that presents several disadvantages: (i) low efficiency, (ii) it is a non-oriented reacti...
| Autores principales: | , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2019
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6670230/ https://www.ncbi.nlm.nih.gov/pubmed/31388344 http://dx.doi.org/10.1186/s13007-019-0470-4 |