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“Mycobacterium mephinesia”, a Mycobacterium terrae complex species of clinical interest isolated in French Polynesia

A 59-year-old tobacco smoker male with chronic bronchitis living in Taravao, French Polynesia, Pacific, presented with a two-year growing nodule in the middle lobe of the right lung. A guided bronchoalveolar lavage inoculated onto Löwenstein-Jensen medium yielded colonies of a rapidly-growing non-ch...

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Autores principales: Saad, Jamal, Phelippeau, Michael, Khoder, May, Lévy, Marc, Musso, Didier, Drancourt, Michel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6671966/
https://www.ncbi.nlm.nih.gov/pubmed/31371776
http://dx.doi.org/10.1038/s41598-019-47674-8
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author Saad, Jamal
Phelippeau, Michael
Khoder, May
Lévy, Marc
Musso, Didier
Drancourt, Michel
author_facet Saad, Jamal
Phelippeau, Michael
Khoder, May
Lévy, Marc
Musso, Didier
Drancourt, Michel
author_sort Saad, Jamal
collection PubMed
description A 59-year-old tobacco smoker male with chronic bronchitis living in Taravao, French Polynesia, Pacific, presented with a two-year growing nodule in the middle lobe of the right lung. A guided bronchoalveolar lavage inoculated onto Löwenstein-Jensen medium yielded colonies of a rapidly-growing non-chromogenic mycobacterium designed as isolate P7213. The isolate could not be identified using routine matrix-assisted laser desorption ionization-time of flight-mass spectrometry and phenotypic and probe-hybridization techniques and yielded 100% and 97% sequence similarity with the respective 16S rRNA and rpoB gene sequences of Mycobacterium virginiense in the Mycobacterium terrae complex. Electron microscopy showed a 1.15 µm long and 0.38 µm large bacillus which was in vitro susceptible to rifampicin, rifabutin, ethambutol, isoniazid, doxycycline and kanamycin. Its 4,511,948-bp draft genome exhibited a 67.6% G + C content with 4,153 coding-protein genes and 87 predicted RNA genes. Genome sequence-derived DNA-DNA hybridization, OrthoANI and pangenome analysis confirmed isolate P7213 was representative of a new species in the M. terrae complex. We named this species “Mycobacterium mephinesia”.
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spelling pubmed-66719662019-08-07 “Mycobacterium mephinesia”, a Mycobacterium terrae complex species of clinical interest isolated in French Polynesia Saad, Jamal Phelippeau, Michael Khoder, May Lévy, Marc Musso, Didier Drancourt, Michel Sci Rep Article A 59-year-old tobacco smoker male with chronic bronchitis living in Taravao, French Polynesia, Pacific, presented with a two-year growing nodule in the middle lobe of the right lung. A guided bronchoalveolar lavage inoculated onto Löwenstein-Jensen medium yielded colonies of a rapidly-growing non-chromogenic mycobacterium designed as isolate P7213. The isolate could not be identified using routine matrix-assisted laser desorption ionization-time of flight-mass spectrometry and phenotypic and probe-hybridization techniques and yielded 100% and 97% sequence similarity with the respective 16S rRNA and rpoB gene sequences of Mycobacterium virginiense in the Mycobacterium terrae complex. Electron microscopy showed a 1.15 µm long and 0.38 µm large bacillus which was in vitro susceptible to rifampicin, rifabutin, ethambutol, isoniazid, doxycycline and kanamycin. Its 4,511,948-bp draft genome exhibited a 67.6% G + C content with 4,153 coding-protein genes and 87 predicted RNA genes. Genome sequence-derived DNA-DNA hybridization, OrthoANI and pangenome analysis confirmed isolate P7213 was representative of a new species in the M. terrae complex. We named this species “Mycobacterium mephinesia”. Nature Publishing Group UK 2019-08-01 /pmc/articles/PMC6671966/ /pubmed/31371776 http://dx.doi.org/10.1038/s41598-019-47674-8 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Saad, Jamal
Phelippeau, Michael
Khoder, May
Lévy, Marc
Musso, Didier
Drancourt, Michel
“Mycobacterium mephinesia”, a Mycobacterium terrae complex species of clinical interest isolated in French Polynesia
title “Mycobacterium mephinesia”, a Mycobacterium terrae complex species of clinical interest isolated in French Polynesia
title_full “Mycobacterium mephinesia”, a Mycobacterium terrae complex species of clinical interest isolated in French Polynesia
title_fullStr “Mycobacterium mephinesia”, a Mycobacterium terrae complex species of clinical interest isolated in French Polynesia
title_full_unstemmed “Mycobacterium mephinesia”, a Mycobacterium terrae complex species of clinical interest isolated in French Polynesia
title_short “Mycobacterium mephinesia”, a Mycobacterium terrae complex species of clinical interest isolated in French Polynesia
title_sort “mycobacterium mephinesia”, a mycobacterium terrae complex species of clinical interest isolated in french polynesia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6671966/
https://www.ncbi.nlm.nih.gov/pubmed/31371776
http://dx.doi.org/10.1038/s41598-019-47674-8
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