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Neurons-derived extracellular vesicles promote neural differentiation of ADSCs: a model to prevent peripheral nerve degeneration

Potential mechanisms involved in neural differentiation of adipocyte derived stem cells (ADSCs) are still unclear. In the present study, extracellular vesicles (EVs) were tested as a potential mechanism involved in the neuronal differentiation of stem cells. In order to address this, ADSCs and neuro...

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Autores principales: Roballo, Kelly Cristine Santos, da Silveira, Juliano Coelho, Bressan, Fabiana Fernandes, de Souza, Aline Fernanda, Pereira, Vitoria Mattos, Porras, Jorge Eliecer Pinzon, Rós, Felipe Augusto, Pulz, Lidia Hildebrand, Strefezzi, Ricardo de Francisco, Martins, Daniele dos Santos, Meirelles, Flavio Vieira, Ambrósio, Carlos Eduardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6671995/
https://www.ncbi.nlm.nih.gov/pubmed/31371742
http://dx.doi.org/10.1038/s41598-019-47229-x
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author Roballo, Kelly Cristine Santos
da Silveira, Juliano Coelho
Bressan, Fabiana Fernandes
de Souza, Aline Fernanda
Pereira, Vitoria Mattos
Porras, Jorge Eliecer Pinzon
Rós, Felipe Augusto
Pulz, Lidia Hildebrand
Strefezzi, Ricardo de Francisco
Martins, Daniele dos Santos
Meirelles, Flavio Vieira
Ambrósio, Carlos Eduardo
author_facet Roballo, Kelly Cristine Santos
da Silveira, Juliano Coelho
Bressan, Fabiana Fernandes
de Souza, Aline Fernanda
Pereira, Vitoria Mattos
Porras, Jorge Eliecer Pinzon
Rós, Felipe Augusto
Pulz, Lidia Hildebrand
Strefezzi, Ricardo de Francisco
Martins, Daniele dos Santos
Meirelles, Flavio Vieira
Ambrósio, Carlos Eduardo
author_sort Roballo, Kelly Cristine Santos
collection PubMed
description Potential mechanisms involved in neural differentiation of adipocyte derived stem cells (ADSCs) are still unclear. In the present study, extracellular vesicles (EVs) were tested as a potential mechanism involved in the neuronal differentiation of stem cells. In order to address this, ADSCs and neurons (BRC) were established in primary culture and co-culture at three timepoints. Furthermore, we evaluated protein and transcript levels of differentiated ADSCs from the same timepoints, to confirm phenotype change to neuronal linage. Importantly, neuron-derived EVs cargo and EVs originated from co-culture were analyzed and tested in terms of function, such as gene expression and microRNA levels related to the adult neurogenesis process. Ideal neuron-like cells were identified and, therefore, we speculated the in vivo function of these cells in acute sciatic nerve injury. Overall, our data demonstrated that ADSCs in indirect contact with neurons differentiated into neuron-like cells. Neuron-derived EVs appear to play an important role in this process carrying SNAP25, miR-132 and miR-9. Additionally, in vivo neuron-like cells helped in microenvironment modulation probably preventing peripheral nerve injury degeneration. Consequently, our findings provide new insight of future methods of ADSC induction into neuronal linage to be applied in peripheral nerve (PN) injury.
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spelling pubmed-66719952019-08-07 Neurons-derived extracellular vesicles promote neural differentiation of ADSCs: a model to prevent peripheral nerve degeneration Roballo, Kelly Cristine Santos da Silveira, Juliano Coelho Bressan, Fabiana Fernandes de Souza, Aline Fernanda Pereira, Vitoria Mattos Porras, Jorge Eliecer Pinzon Rós, Felipe Augusto Pulz, Lidia Hildebrand Strefezzi, Ricardo de Francisco Martins, Daniele dos Santos Meirelles, Flavio Vieira Ambrósio, Carlos Eduardo Sci Rep Article Potential mechanisms involved in neural differentiation of adipocyte derived stem cells (ADSCs) are still unclear. In the present study, extracellular vesicles (EVs) were tested as a potential mechanism involved in the neuronal differentiation of stem cells. In order to address this, ADSCs and neurons (BRC) were established in primary culture and co-culture at three timepoints. Furthermore, we evaluated protein and transcript levels of differentiated ADSCs from the same timepoints, to confirm phenotype change to neuronal linage. Importantly, neuron-derived EVs cargo and EVs originated from co-culture were analyzed and tested in terms of function, such as gene expression and microRNA levels related to the adult neurogenesis process. Ideal neuron-like cells were identified and, therefore, we speculated the in vivo function of these cells in acute sciatic nerve injury. Overall, our data demonstrated that ADSCs in indirect contact with neurons differentiated into neuron-like cells. Neuron-derived EVs appear to play an important role in this process carrying SNAP25, miR-132 and miR-9. Additionally, in vivo neuron-like cells helped in microenvironment modulation probably preventing peripheral nerve injury degeneration. Consequently, our findings provide new insight of future methods of ADSC induction into neuronal linage to be applied in peripheral nerve (PN) injury. Nature Publishing Group UK 2019-08-01 /pmc/articles/PMC6671995/ /pubmed/31371742 http://dx.doi.org/10.1038/s41598-019-47229-x Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Roballo, Kelly Cristine Santos
da Silveira, Juliano Coelho
Bressan, Fabiana Fernandes
de Souza, Aline Fernanda
Pereira, Vitoria Mattos
Porras, Jorge Eliecer Pinzon
Rós, Felipe Augusto
Pulz, Lidia Hildebrand
Strefezzi, Ricardo de Francisco
Martins, Daniele dos Santos
Meirelles, Flavio Vieira
Ambrósio, Carlos Eduardo
Neurons-derived extracellular vesicles promote neural differentiation of ADSCs: a model to prevent peripheral nerve degeneration
title Neurons-derived extracellular vesicles promote neural differentiation of ADSCs: a model to prevent peripheral nerve degeneration
title_full Neurons-derived extracellular vesicles promote neural differentiation of ADSCs: a model to prevent peripheral nerve degeneration
title_fullStr Neurons-derived extracellular vesicles promote neural differentiation of ADSCs: a model to prevent peripheral nerve degeneration
title_full_unstemmed Neurons-derived extracellular vesicles promote neural differentiation of ADSCs: a model to prevent peripheral nerve degeneration
title_short Neurons-derived extracellular vesicles promote neural differentiation of ADSCs: a model to prevent peripheral nerve degeneration
title_sort neurons-derived extracellular vesicles promote neural differentiation of adscs: a model to prevent peripheral nerve degeneration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6671995/
https://www.ncbi.nlm.nih.gov/pubmed/31371742
http://dx.doi.org/10.1038/s41598-019-47229-x
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