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Measuring light transport properties using speckle patterns as structured illumination
The measurement of light absorption and scattering properties of biological materials has several diagnostic and therapeutic applications. We can measure these properties for skin without contact using structured illumination and imaging. However, building simple handheld devices remains challenging...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6672017/ https://www.ncbi.nlm.nih.gov/pubmed/31371799 http://dx.doi.org/10.1038/s41598-019-47256-8 |
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author | Jain, Pranay Sarma, Sanjay E. |
author_facet | Jain, Pranay Sarma, Sanjay E. |
author_sort | Jain, Pranay |
collection | PubMed |
description | The measurement of light absorption and scattering properties of biological materials has several diagnostic and therapeutic applications. We can measure these properties for skin without contact using structured illumination and imaging. However, building simple handheld devices remains challenging due to motion artefacts and moving targets. To overcome this limitation, we project random speckle patterns instead of discrete spatial frequencies on the target. Since random patterns are spatially broadband, they capture more information per image, enabling frame-by-frame analysis. In this paper, we describe the statistics of objective speckles and demonstrate how the optical system is designed for spatially bandlimited illumination. Next, we use a diverse set of liquid tissue phantom to validate the method. We successfully demonstrate that a calibrated instrument can independently predict the two primary light transport properties of a homogeneous turbid system. This work is a starting point for analysing skin and other heterogeneous biological media in the future. |
format | Online Article Text |
id | pubmed-6672017 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-66720172019-08-07 Measuring light transport properties using speckle patterns as structured illumination Jain, Pranay Sarma, Sanjay E. Sci Rep Article The measurement of light absorption and scattering properties of biological materials has several diagnostic and therapeutic applications. We can measure these properties for skin without contact using structured illumination and imaging. However, building simple handheld devices remains challenging due to motion artefacts and moving targets. To overcome this limitation, we project random speckle patterns instead of discrete spatial frequencies on the target. Since random patterns are spatially broadband, they capture more information per image, enabling frame-by-frame analysis. In this paper, we describe the statistics of objective speckles and demonstrate how the optical system is designed for spatially bandlimited illumination. Next, we use a diverse set of liquid tissue phantom to validate the method. We successfully demonstrate that a calibrated instrument can independently predict the two primary light transport properties of a homogeneous turbid system. This work is a starting point for analysing skin and other heterogeneous biological media in the future. Nature Publishing Group UK 2019-08-01 /pmc/articles/PMC6672017/ /pubmed/31371799 http://dx.doi.org/10.1038/s41598-019-47256-8 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Jain, Pranay Sarma, Sanjay E. Measuring light transport properties using speckle patterns as structured illumination |
title | Measuring light transport properties using speckle patterns as structured illumination |
title_full | Measuring light transport properties using speckle patterns as structured illumination |
title_fullStr | Measuring light transport properties using speckle patterns as structured illumination |
title_full_unstemmed | Measuring light transport properties using speckle patterns as structured illumination |
title_short | Measuring light transport properties using speckle patterns as structured illumination |
title_sort | measuring light transport properties using speckle patterns as structured illumination |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6672017/ https://www.ncbi.nlm.nih.gov/pubmed/31371799 http://dx.doi.org/10.1038/s41598-019-47256-8 |
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