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Std fimbriae-fucose interaction increases Salmonella-induced intestinal inflammation and prolongs colonization
Expression of ABO and Lewis histo-blood group antigens by the gastrointestinal epithelium is governed by an α-1,2-fucosyltransferase enzyme encoded by the Fut2 gene. Alterations in mucin glycosylation have been associated with susceptibility to various bacterial and viral infections. Salmonella ente...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6675130/ https://www.ncbi.nlm.nih.gov/pubmed/31329635 http://dx.doi.org/10.1371/journal.ppat.1007915 |
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author | Suwandi, Abdulhadi Galeev, Alibek Riedel, René Sharma, Samriti Seeger, Katrin Sterzenbach, Torsten García Pastor, Lucía Boyle, Erin C. Gal-Mor, Ohad Hensel, Michael Casadesús, Josep Baines, John F. Grassl, Guntram A. |
author_facet | Suwandi, Abdulhadi Galeev, Alibek Riedel, René Sharma, Samriti Seeger, Katrin Sterzenbach, Torsten García Pastor, Lucía Boyle, Erin C. Gal-Mor, Ohad Hensel, Michael Casadesús, Josep Baines, John F. Grassl, Guntram A. |
author_sort | Suwandi, Abdulhadi |
collection | PubMed |
description | Expression of ABO and Lewis histo-blood group antigens by the gastrointestinal epithelium is governed by an α-1,2-fucosyltransferase enzyme encoded by the Fut2 gene. Alterations in mucin glycosylation have been associated with susceptibility to various bacterial and viral infections. Salmonella enterica serovar Typhimurium is a food-borne pathogen and a major cause of gastroenteritis. In order to determine the role of Fut2-dependent glycans in Salmonella-triggered intestinal inflammation, Fut2(+/+) and Fut2(-/-) mice were orally infected with S. Typhimurium and bacterial colonization and intestinal inflammation were analyzed. Bacterial load in the intestine of Fut2(-/-) mice was significantly lower compared to Fut2(+/+) mice. Analysis of histopathological changes revealed significantly lower levels of intestinal inflammation in Fut2(-/-) mice compared to Fut2(+/+) mice and measurement of lipocalin-2 level in feces corroborated histopathological findings. Salmonella express fimbriae that assist in adherence of bacteria to host cells thereby facilitating their invasion. The std fimbrial operon of S. Typhimurium encodes the π-class Std fimbriae which bind terminal α(1,2)-fucose residues. An isogenic mutant of S. Typhimurium lacking Std fimbriae colonized Fut2(+/+) and Fut2(-/-) mice to similar levels and resulted in similar intestinal inflammation. In vitro adhesion assays revealed that bacteria possessing Std fimbriae adhered significantly more to fucosylated cell lines or primary epithelial cells in comparison to cells lacking α(1,2)-fucose. Overall, these results indicate that Salmonella-triggered intestinal inflammation and colonization are dependent on Std-fucose interaction. |
format | Online Article Text |
id | pubmed-6675130 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-66751302019-08-06 Std fimbriae-fucose interaction increases Salmonella-induced intestinal inflammation and prolongs colonization Suwandi, Abdulhadi Galeev, Alibek Riedel, René Sharma, Samriti Seeger, Katrin Sterzenbach, Torsten García Pastor, Lucía Boyle, Erin C. Gal-Mor, Ohad Hensel, Michael Casadesús, Josep Baines, John F. Grassl, Guntram A. PLoS Pathog Research Article Expression of ABO and Lewis histo-blood group antigens by the gastrointestinal epithelium is governed by an α-1,2-fucosyltransferase enzyme encoded by the Fut2 gene. Alterations in mucin glycosylation have been associated with susceptibility to various bacterial and viral infections. Salmonella enterica serovar Typhimurium is a food-borne pathogen and a major cause of gastroenteritis. In order to determine the role of Fut2-dependent glycans in Salmonella-triggered intestinal inflammation, Fut2(+/+) and Fut2(-/-) mice were orally infected with S. Typhimurium and bacterial colonization and intestinal inflammation were analyzed. Bacterial load in the intestine of Fut2(-/-) mice was significantly lower compared to Fut2(+/+) mice. Analysis of histopathological changes revealed significantly lower levels of intestinal inflammation in Fut2(-/-) mice compared to Fut2(+/+) mice and measurement of lipocalin-2 level in feces corroborated histopathological findings. Salmonella express fimbriae that assist in adherence of bacteria to host cells thereby facilitating their invasion. The std fimbrial operon of S. Typhimurium encodes the π-class Std fimbriae which bind terminal α(1,2)-fucose residues. An isogenic mutant of S. Typhimurium lacking Std fimbriae colonized Fut2(+/+) and Fut2(-/-) mice to similar levels and resulted in similar intestinal inflammation. In vitro adhesion assays revealed that bacteria possessing Std fimbriae adhered significantly more to fucosylated cell lines or primary epithelial cells in comparison to cells lacking α(1,2)-fucose. Overall, these results indicate that Salmonella-triggered intestinal inflammation and colonization are dependent on Std-fucose interaction. Public Library of Science 2019-07-22 /pmc/articles/PMC6675130/ /pubmed/31329635 http://dx.doi.org/10.1371/journal.ppat.1007915 Text en © 2019 Suwandi et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Suwandi, Abdulhadi Galeev, Alibek Riedel, René Sharma, Samriti Seeger, Katrin Sterzenbach, Torsten García Pastor, Lucía Boyle, Erin C. Gal-Mor, Ohad Hensel, Michael Casadesús, Josep Baines, John F. Grassl, Guntram A. Std fimbriae-fucose interaction increases Salmonella-induced intestinal inflammation and prolongs colonization |
title | Std fimbriae-fucose interaction increases Salmonella-induced intestinal inflammation and prolongs colonization |
title_full | Std fimbriae-fucose interaction increases Salmonella-induced intestinal inflammation and prolongs colonization |
title_fullStr | Std fimbriae-fucose interaction increases Salmonella-induced intestinal inflammation and prolongs colonization |
title_full_unstemmed | Std fimbriae-fucose interaction increases Salmonella-induced intestinal inflammation and prolongs colonization |
title_short | Std fimbriae-fucose interaction increases Salmonella-induced intestinal inflammation and prolongs colonization |
title_sort | std fimbriae-fucose interaction increases salmonella-induced intestinal inflammation and prolongs colonization |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6675130/ https://www.ncbi.nlm.nih.gov/pubmed/31329635 http://dx.doi.org/10.1371/journal.ppat.1007915 |
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