Cargando…

Self-capping of nucleoprotein filaments protects the Newcastle disease virus genome

Non-segmented negative-strand RNA viruses, such as measles, ebola and Newcastle disease viruses (NDV), encapsidate viral genomic RNAs into helical nucleocapsids, which serve as the template for viral replication and transcription. Here, the clam-shaped nucleocapsid structure, where the NDV viral gen...

Descripción completa

Detalles Bibliográficos
Autores principales: Song, Xiyong, Shan, Hong, Zhu, Yanping, Hu, Shunlin, Xue, Ling, Chen, Yong, Ding, Wei, Niu, Tongxin, Gu, Jian, Ouyang, Songying, Shen, Qing-Tao, Liu, Zhi-Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6675542/
https://www.ncbi.nlm.nih.gov/pubmed/31290740
http://dx.doi.org/10.7554/eLife.45057
_version_ 1783440640006160384
author Song, Xiyong
Shan, Hong
Zhu, Yanping
Hu, Shunlin
Xue, Ling
Chen, Yong
Ding, Wei
Niu, Tongxin
Gu, Jian
Ouyang, Songying
Shen, Qing-Tao
Liu, Zhi-Jie
author_facet Song, Xiyong
Shan, Hong
Zhu, Yanping
Hu, Shunlin
Xue, Ling
Chen, Yong
Ding, Wei
Niu, Tongxin
Gu, Jian
Ouyang, Songying
Shen, Qing-Tao
Liu, Zhi-Jie
author_sort Song, Xiyong
collection PubMed
description Non-segmented negative-strand RNA viruses, such as measles, ebola and Newcastle disease viruses (NDV), encapsidate viral genomic RNAs into helical nucleocapsids, which serve as the template for viral replication and transcription. Here, the clam-shaped nucleocapsid structure, where the NDV viral genome is sequestered, was determined at 4.8 Å resolution by cryo-electron microscopy. The clam-shaped structure is composed of two single-turn spirals packed in a back-to-back mode. This tightly packed structure functions as a seed for the assembly of a nucleocapsid from both directions, facilitating the growth of double-headed filaments with two separate RNA strings inside. Disruption of this structure by mutations in its loop interface yielded a single-headed unfunctional filament.
format Online
Article
Text
id pubmed-6675542
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher eLife Sciences Publications, Ltd
record_format MEDLINE/PubMed
spelling pubmed-66755422019-08-05 Self-capping of nucleoprotein filaments protects the Newcastle disease virus genome Song, Xiyong Shan, Hong Zhu, Yanping Hu, Shunlin Xue, Ling Chen, Yong Ding, Wei Niu, Tongxin Gu, Jian Ouyang, Songying Shen, Qing-Tao Liu, Zhi-Jie eLife Microbiology and Infectious Disease Non-segmented negative-strand RNA viruses, such as measles, ebola and Newcastle disease viruses (NDV), encapsidate viral genomic RNAs into helical nucleocapsids, which serve as the template for viral replication and transcription. Here, the clam-shaped nucleocapsid structure, where the NDV viral genome is sequestered, was determined at 4.8 Å resolution by cryo-electron microscopy. The clam-shaped structure is composed of two single-turn spirals packed in a back-to-back mode. This tightly packed structure functions as a seed for the assembly of a nucleocapsid from both directions, facilitating the growth of double-headed filaments with two separate RNA strings inside. Disruption of this structure by mutations in its loop interface yielded a single-headed unfunctional filament. eLife Sciences Publications, Ltd 2019-07-10 /pmc/articles/PMC6675542/ /pubmed/31290740 http://dx.doi.org/10.7554/eLife.45057 Text en © 2019, Song et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Microbiology and Infectious Disease
Song, Xiyong
Shan, Hong
Zhu, Yanping
Hu, Shunlin
Xue, Ling
Chen, Yong
Ding, Wei
Niu, Tongxin
Gu, Jian
Ouyang, Songying
Shen, Qing-Tao
Liu, Zhi-Jie
Self-capping of nucleoprotein filaments protects the Newcastle disease virus genome
title Self-capping of nucleoprotein filaments protects the Newcastle disease virus genome
title_full Self-capping of nucleoprotein filaments protects the Newcastle disease virus genome
title_fullStr Self-capping of nucleoprotein filaments protects the Newcastle disease virus genome
title_full_unstemmed Self-capping of nucleoprotein filaments protects the Newcastle disease virus genome
title_short Self-capping of nucleoprotein filaments protects the Newcastle disease virus genome
title_sort self-capping of nucleoprotein filaments protects the newcastle disease virus genome
topic Microbiology and Infectious Disease
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6675542/
https://www.ncbi.nlm.nih.gov/pubmed/31290740
http://dx.doi.org/10.7554/eLife.45057
work_keys_str_mv AT songxiyong selfcappingofnucleoproteinfilamentsprotectsthenewcastlediseasevirusgenome
AT shanhong selfcappingofnucleoproteinfilamentsprotectsthenewcastlediseasevirusgenome
AT zhuyanping selfcappingofnucleoproteinfilamentsprotectsthenewcastlediseasevirusgenome
AT hushunlin selfcappingofnucleoproteinfilamentsprotectsthenewcastlediseasevirusgenome
AT xueling selfcappingofnucleoproteinfilamentsprotectsthenewcastlediseasevirusgenome
AT chenyong selfcappingofnucleoproteinfilamentsprotectsthenewcastlediseasevirusgenome
AT dingwei selfcappingofnucleoproteinfilamentsprotectsthenewcastlediseasevirusgenome
AT niutongxin selfcappingofnucleoproteinfilamentsprotectsthenewcastlediseasevirusgenome
AT gujian selfcappingofnucleoproteinfilamentsprotectsthenewcastlediseasevirusgenome
AT ouyangsongying selfcappingofnucleoproteinfilamentsprotectsthenewcastlediseasevirusgenome
AT shenqingtao selfcappingofnucleoproteinfilamentsprotectsthenewcastlediseasevirusgenome
AT liuzhijie selfcappingofnucleoproteinfilamentsprotectsthenewcastlediseasevirusgenome