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Downregulation of miR-3934-5p enhances A549 cell sensitivity to cisplatin by targeting TP53INP1

Tumor protein p53-inducible nuclear protein 1 (TP53INP1) is a tumor suppressor associated with malignant tumor metastasis. In addition, it has been reported that hsa-microRNA (miR)-3934 serves key roles in various types of lung cancer, including small-cell lung carcinomas (SCLC) and non-SCLC (NSCLC)...

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Autores principales: Ren, Aijun, Wen, Zhenzhen, Zheng, Liangjie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6676217/
https://www.ncbi.nlm.nih.gov/pubmed/31410122
http://dx.doi.org/10.3892/etm.2019.7718
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author Ren, Aijun
Wen, Zhenzhen
Zheng, Liangjie
author_facet Ren, Aijun
Wen, Zhenzhen
Zheng, Liangjie
author_sort Ren, Aijun
collection PubMed
description Tumor protein p53-inducible nuclear protein 1 (TP53INP1) is a tumor suppressor associated with malignant tumor metastasis. In addition, it has been reported that hsa-microRNA (miR)-3934 serves key roles in various types of lung cancer, including small-cell lung carcinomas (SCLC) and non-SCLC (NSCLC). Therefore, the present study aimed to determine the effects of miR-3934-5p on cell proliferation and apoptosis, and on sensitivity to cisplatin (DDP). Reverse transcription-quantitative polymerase chain reaction analysis and western blotting were conducted for the analysis of mRNA and protein expression, respectively. Furthermore, the target of miR-3934-5p was investigated using a luciferase reporter assay and apoptosis was analyzed by flow cytometry. The results demonstrated that miR-3934-5p was upregulated in NSCLC tissues and A549 cells. Increases in the half-maximal inhibitory concentration (IC(50)) and the expression of miR-3934-5p were observed in the A549/DDP group. miR-3934-5p mimic promoted the expression of miR-3934-5p and the IC(50) of the A549 cells. miR-3934-5p inhibitor downregulated miR-3934-5p and reduced the IC(50) of A549/DDP cells. miR-3934-5p was revealed to target the 3′-untranslated region of TP53INP1. The downregulation of miR-3934-5p significantly suppressed the proliferation and promoted the apoptosis of A549/DDP cells, which were reversed by transfection with TP53INP1 small interfering (si)RNA. The protein and mRNA expression levels of TP53INP1, B-cell lymphoma 2 (Bcl-2)-associated-X and p21 were significantly increased, whereas those of Bcl-2 were significantly decreased in the miR-3934-5p inhibitor group, which was significantly reduced by TP53INP1 siRNA transfection. miR-3934-5p, as a tumor suppressor in NSCLC, may promote the sensitivity of cells to DDP by targeting TP53INP1, associated with the suppression of cell proliferation and promotion of apoptosis.
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spelling pubmed-66762172019-08-13 Downregulation of miR-3934-5p enhances A549 cell sensitivity to cisplatin by targeting TP53INP1 Ren, Aijun Wen, Zhenzhen Zheng, Liangjie Exp Ther Med Articles Tumor protein p53-inducible nuclear protein 1 (TP53INP1) is a tumor suppressor associated with malignant tumor metastasis. In addition, it has been reported that hsa-microRNA (miR)-3934 serves key roles in various types of lung cancer, including small-cell lung carcinomas (SCLC) and non-SCLC (NSCLC). Therefore, the present study aimed to determine the effects of miR-3934-5p on cell proliferation and apoptosis, and on sensitivity to cisplatin (DDP). Reverse transcription-quantitative polymerase chain reaction analysis and western blotting were conducted for the analysis of mRNA and protein expression, respectively. Furthermore, the target of miR-3934-5p was investigated using a luciferase reporter assay and apoptosis was analyzed by flow cytometry. The results demonstrated that miR-3934-5p was upregulated in NSCLC tissues and A549 cells. Increases in the half-maximal inhibitory concentration (IC(50)) and the expression of miR-3934-5p were observed in the A549/DDP group. miR-3934-5p mimic promoted the expression of miR-3934-5p and the IC(50) of the A549 cells. miR-3934-5p inhibitor downregulated miR-3934-5p and reduced the IC(50) of A549/DDP cells. miR-3934-5p was revealed to target the 3′-untranslated region of TP53INP1. The downregulation of miR-3934-5p significantly suppressed the proliferation and promoted the apoptosis of A549/DDP cells, which were reversed by transfection with TP53INP1 small interfering (si)RNA. The protein and mRNA expression levels of TP53INP1, B-cell lymphoma 2 (Bcl-2)-associated-X and p21 were significantly increased, whereas those of Bcl-2 were significantly decreased in the miR-3934-5p inhibitor group, which was significantly reduced by TP53INP1 siRNA transfection. miR-3934-5p, as a tumor suppressor in NSCLC, may promote the sensitivity of cells to DDP by targeting TP53INP1, associated with the suppression of cell proliferation and promotion of apoptosis. D.A. Spandidos 2019-09 2019-07-01 /pmc/articles/PMC6676217/ /pubmed/31410122 http://dx.doi.org/10.3892/etm.2019.7718 Text en Copyright: © Ren et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Ren, Aijun
Wen, Zhenzhen
Zheng, Liangjie
Downregulation of miR-3934-5p enhances A549 cell sensitivity to cisplatin by targeting TP53INP1
title Downregulation of miR-3934-5p enhances A549 cell sensitivity to cisplatin by targeting TP53INP1
title_full Downregulation of miR-3934-5p enhances A549 cell sensitivity to cisplatin by targeting TP53INP1
title_fullStr Downregulation of miR-3934-5p enhances A549 cell sensitivity to cisplatin by targeting TP53INP1
title_full_unstemmed Downregulation of miR-3934-5p enhances A549 cell sensitivity to cisplatin by targeting TP53INP1
title_short Downregulation of miR-3934-5p enhances A549 cell sensitivity to cisplatin by targeting TP53INP1
title_sort downregulation of mir-3934-5p enhances a549 cell sensitivity to cisplatin by targeting tp53inp1
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6676217/
https://www.ncbi.nlm.nih.gov/pubmed/31410122
http://dx.doi.org/10.3892/etm.2019.7718
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