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Lack of specificity associated with using molecular beacons in loop mediated amplification assays

BACKGROUND: Loop mediated isothermal amplification of nucleic acid templates is a rapid, sensitive and specific method suitable for molecular diagnostics. However the complexity of primer design and the number of primers involved can lead to false positives from non-specific primer interactions. Sta...

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Detalles Bibliográficos
Autores principales: Hardinge, Patrick, Murray, James A. H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6676609/
https://www.ncbi.nlm.nih.gov/pubmed/31370820
http://dx.doi.org/10.1186/s12896-019-0549-z

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