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Lack of specificity associated with using molecular beacons in loop mediated amplification assays
BACKGROUND: Loop mediated isothermal amplification of nucleic acid templates is a rapid, sensitive and specific method suitable for molecular diagnostics. However the complexity of primer design and the number of primers involved can lead to false positives from non-specific primer interactions. Sta...
Autores principales: | Hardinge, Patrick, Murray, James A. H. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6676609/ https://www.ncbi.nlm.nih.gov/pubmed/31370820 http://dx.doi.org/10.1186/s12896-019-0549-z |
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