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miR-148a inhibits cell proliferation and migration through targeting ErbB3 in colorectal cancer

Colorectal cancer is a common gastrointestinal cancer ranking in third place of all cancers. Downregulation of miR-148a has been observed in many tumors, and miR-148a was found to be an oncogene in colorectal cancer. The aim of our study was to investigate the molecular mechanisms by which miR-148a...

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Autores principales: Zhao, Wei, Zheng, Jianbao, Wei, Guangbing, Yang, Kui, Wang, Guanghui, Sun, Xuejun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6676750/
https://www.ncbi.nlm.nih.gov/pubmed/31402949
http://dx.doi.org/10.3892/ol.2019.10581
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author Zhao, Wei
Zheng, Jianbao
Wei, Guangbing
Yang, Kui
Wang, Guanghui
Sun, Xuejun
author_facet Zhao, Wei
Zheng, Jianbao
Wei, Guangbing
Yang, Kui
Wang, Guanghui
Sun, Xuejun
author_sort Zhao, Wei
collection PubMed
description Colorectal cancer is a common gastrointestinal cancer ranking in third place of all cancers. Downregulation of miR-148a has been observed in many tumors, and miR-148a was found to be an oncogene in colorectal cancer. The aim of our study was to investigate the molecular mechanisms by which miR-148a and ErbB3 proliferate and migrate in colorectal cancer. The expression of miR-148a and ErbB3 were measured by western blot analysis and RT-qPCR. MTT and transwell assays were performed to analyze the proliferative and migratory abilities. The dual luciferase reporter assay was employed to confirm miR-148a regulated the expression of ErbB3 in colorectal cancer. It was discovered that miR-148a was overexpressed while ErbB3 expression was low in colorectal cancer, and the mRNA level of miR-148a had a negative correlation with the expression of ErbB3. Upregulation of miR-148a suppressed the proliferation and migration in colorectal cancer cells. Furthermore, ErbB3 was identified as a direct target of miR-148a, which suppressed the proliferation and migration through directly binding to the 3′UTR of ErbB3 mRNA. This study established that miR-148a inhibited the proliferative and migratory abilities through mediating the expression of ErbB3. The newly identified miR-148a/ErbB3 axis provides novel insight into the pathogenesis of colorectal cancer, and represents a potential target for treatment of colorectal cancer.
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spelling pubmed-66767502019-08-09 miR-148a inhibits cell proliferation and migration through targeting ErbB3 in colorectal cancer Zhao, Wei Zheng, Jianbao Wei, Guangbing Yang, Kui Wang, Guanghui Sun, Xuejun Oncol Lett Articles Colorectal cancer is a common gastrointestinal cancer ranking in third place of all cancers. Downregulation of miR-148a has been observed in many tumors, and miR-148a was found to be an oncogene in colorectal cancer. The aim of our study was to investigate the molecular mechanisms by which miR-148a and ErbB3 proliferate and migrate in colorectal cancer. The expression of miR-148a and ErbB3 were measured by western blot analysis and RT-qPCR. MTT and transwell assays were performed to analyze the proliferative and migratory abilities. The dual luciferase reporter assay was employed to confirm miR-148a regulated the expression of ErbB3 in colorectal cancer. It was discovered that miR-148a was overexpressed while ErbB3 expression was low in colorectal cancer, and the mRNA level of miR-148a had a negative correlation with the expression of ErbB3. Upregulation of miR-148a suppressed the proliferation and migration in colorectal cancer cells. Furthermore, ErbB3 was identified as a direct target of miR-148a, which suppressed the proliferation and migration through directly binding to the 3′UTR of ErbB3 mRNA. This study established that miR-148a inhibited the proliferative and migratory abilities through mediating the expression of ErbB3. The newly identified miR-148a/ErbB3 axis provides novel insight into the pathogenesis of colorectal cancer, and represents a potential target for treatment of colorectal cancer. D.A. Spandidos 2019-09 2019-07-05 /pmc/articles/PMC6676750/ /pubmed/31402949 http://dx.doi.org/10.3892/ol.2019.10581 Text en Copyright: © Zhao et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Zhao, Wei
Zheng, Jianbao
Wei, Guangbing
Yang, Kui
Wang, Guanghui
Sun, Xuejun
miR-148a inhibits cell proliferation and migration through targeting ErbB3 in colorectal cancer
title miR-148a inhibits cell proliferation and migration through targeting ErbB3 in colorectal cancer
title_full miR-148a inhibits cell proliferation and migration through targeting ErbB3 in colorectal cancer
title_fullStr miR-148a inhibits cell proliferation and migration through targeting ErbB3 in colorectal cancer
title_full_unstemmed miR-148a inhibits cell proliferation and migration through targeting ErbB3 in colorectal cancer
title_short miR-148a inhibits cell proliferation and migration through targeting ErbB3 in colorectal cancer
title_sort mir-148a inhibits cell proliferation and migration through targeting erbb3 in colorectal cancer
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6676750/
https://www.ncbi.nlm.nih.gov/pubmed/31402949
http://dx.doi.org/10.3892/ol.2019.10581
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