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Low-cost solution for rodent home-cage behaviour monitoring

In the current research on measuring complex behaviours/phenotyping in rodents, most of the experimental design requires the experimenter to remove the animal from its home-cage environment and place it in an unfamiliar apparatus (novel environment). This interaction may influence behaviour, general...

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Detalles Bibliográficos
Autores principales: Singh, Surjeet, Bermudez-Contreras, Edgar, Nazari, Mojtaba, Sutherland, Robert J., Mohajerani, Majid H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6677321/
https://www.ncbi.nlm.nih.gov/pubmed/31374097
http://dx.doi.org/10.1371/journal.pone.0220751
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author Singh, Surjeet
Bermudez-Contreras, Edgar
Nazari, Mojtaba
Sutherland, Robert J.
Mohajerani, Majid H.
author_facet Singh, Surjeet
Bermudez-Contreras, Edgar
Nazari, Mojtaba
Sutherland, Robert J.
Mohajerani, Majid H.
author_sort Singh, Surjeet
collection PubMed
description In the current research on measuring complex behaviours/phenotyping in rodents, most of the experimental design requires the experimenter to remove the animal from its home-cage environment and place it in an unfamiliar apparatus (novel environment). This interaction may influence behaviour, general well-being, and the metabolism of the animal, affecting the phenotypic outcome even if the data collection method is automated. Most of the commercially available solutions for home-cage monitoring are expensive and usually lack the flexibility to be incorporated with existing home-cages. Here we present a low-cost solution for monitoring home-cage behaviour of rodents that can be easily incorporated to practically any available rodent home-cage. To demonstrate the use of our system, we reliably predict the sleep/wake state of mice in their home-cage using only video. We validate these results using hippocampal local field potential (LFP) and electromyography (EMG) data. Our approach provides a low-cost flexible methodology for high-throughput studies of sleep, circadian rhythm and rodent behaviour with minimal experimenter interference.
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spelling pubmed-66773212019-08-06 Low-cost solution for rodent home-cage behaviour monitoring Singh, Surjeet Bermudez-Contreras, Edgar Nazari, Mojtaba Sutherland, Robert J. Mohajerani, Majid H. PLoS One Research Article In the current research on measuring complex behaviours/phenotyping in rodents, most of the experimental design requires the experimenter to remove the animal from its home-cage environment and place it in an unfamiliar apparatus (novel environment). This interaction may influence behaviour, general well-being, and the metabolism of the animal, affecting the phenotypic outcome even if the data collection method is automated. Most of the commercially available solutions for home-cage monitoring are expensive and usually lack the flexibility to be incorporated with existing home-cages. Here we present a low-cost solution for monitoring home-cage behaviour of rodents that can be easily incorporated to practically any available rodent home-cage. To demonstrate the use of our system, we reliably predict the sleep/wake state of mice in their home-cage using only video. We validate these results using hippocampal local field potential (LFP) and electromyography (EMG) data. Our approach provides a low-cost flexible methodology for high-throughput studies of sleep, circadian rhythm and rodent behaviour with minimal experimenter interference. Public Library of Science 2019-08-02 /pmc/articles/PMC6677321/ /pubmed/31374097 http://dx.doi.org/10.1371/journal.pone.0220751 Text en © 2019 Singh et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Singh, Surjeet
Bermudez-Contreras, Edgar
Nazari, Mojtaba
Sutherland, Robert J.
Mohajerani, Majid H.
Low-cost solution for rodent home-cage behaviour monitoring
title Low-cost solution for rodent home-cage behaviour monitoring
title_full Low-cost solution for rodent home-cage behaviour monitoring
title_fullStr Low-cost solution for rodent home-cage behaviour monitoring
title_full_unstemmed Low-cost solution for rodent home-cage behaviour monitoring
title_short Low-cost solution for rodent home-cage behaviour monitoring
title_sort low-cost solution for rodent home-cage behaviour monitoring
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6677321/
https://www.ncbi.nlm.nih.gov/pubmed/31374097
http://dx.doi.org/10.1371/journal.pone.0220751
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