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Degron-tagged reporters probe membrane topology and enable the specific labelling of membrane-wrapped structures
Visualization of specific organelles in tissues over background fluorescence can be challenging, especially when reporters localize to multiple structures. Instead of trying to identify proteins enriched in specific membrane-wrapped structures, we use a selective degradation approach to remove repor...
Autores principales: | Beer, Katharina B., Fazeli, Gholamreza, Judasova, Kristyna, Irmisch, Linda, Causemann, Jona, Mansfeld, Jörg, Wehman, Ann M. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6677802/ https://www.ncbi.nlm.nih.gov/pubmed/31375709 http://dx.doi.org/10.1038/s41467-019-11442-z |
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