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Proteomic analysis of the aqueous humour in eyes with pseudoexfoliation syndrome
BACKGROUND/AIMS: Pseudoexfoliation syndrome (PEX) is characterised by the production and accumulation of extracellular fibrillar material in the anterior segment of the eye. The pathogenesis of PEX is multifactorial with genetic factors and ageing as contributing factors. Previously, an increased co...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BMJ Publishing Group
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6678048/ https://www.ncbi.nlm.nih.gov/pubmed/30842085 http://dx.doi.org/10.1136/bjophthalmol-2017-310416 |
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author | Botling Taube, Amelie Konzer, Anne Alm, Albert Bergquist, Jonas |
author_facet | Botling Taube, Amelie Konzer, Anne Alm, Albert Bergquist, Jonas |
author_sort | Botling Taube, Amelie |
collection | PubMed |
description | BACKGROUND/AIMS: Pseudoexfoliation syndrome (PEX) is characterised by the production and accumulation of extracellular fibrillar material in the anterior segment of the eye. The pathogenesis of PEX is multifactorial with genetic factors and ageing as contributing factors. Previously, an increased concentration of beta-crystalline B2 (CRYBB2) was observed in the aqueous humour (AH) in eyes with PEX in a pooled material. Here, the protein content was examined on individual basis. METHODS: During cataract surgery, AH was sampled from patients with and without PEX, 10 eyes in each group. The proteins were digested and labelled with isotopomeric dimethyl labels, separated with high-pressure liquid chromatography and analysed in an Orbitrap mass analyzer. RESULTS: The concentration of complement factor 3, kininogen-1, antithrombin III and vitamin D-binding protein was increased in all eyes with PEX. Retinol-binding protein 3, glutathione peroxidase, calsyntenin-1 and carboxypeptidase E were decreased in eyes with PEX. Beta-crystalline B1 and CRYBB2 and gamma-crystalline D were up to eightfold upregulated in 4 of 10 in eyes with PEX. CONCLUSION: The results indicate that oxidative stress and inflammation are contributing factors in the formation of PEX. Knowledge about the proteome in PEX is relevant for understanding this condition. |
format | Online Article Text |
id | pubmed-6678048 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BMJ Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-66780482019-08-16 Proteomic analysis of the aqueous humour in eyes with pseudoexfoliation syndrome Botling Taube, Amelie Konzer, Anne Alm, Albert Bergquist, Jonas Br J Ophthalmol Laboratory Science BACKGROUND/AIMS: Pseudoexfoliation syndrome (PEX) is characterised by the production and accumulation of extracellular fibrillar material in the anterior segment of the eye. The pathogenesis of PEX is multifactorial with genetic factors and ageing as contributing factors. Previously, an increased concentration of beta-crystalline B2 (CRYBB2) was observed in the aqueous humour (AH) in eyes with PEX in a pooled material. Here, the protein content was examined on individual basis. METHODS: During cataract surgery, AH was sampled from patients with and without PEX, 10 eyes in each group. The proteins were digested and labelled with isotopomeric dimethyl labels, separated with high-pressure liquid chromatography and analysed in an Orbitrap mass analyzer. RESULTS: The concentration of complement factor 3, kininogen-1, antithrombin III and vitamin D-binding protein was increased in all eyes with PEX. Retinol-binding protein 3, glutathione peroxidase, calsyntenin-1 and carboxypeptidase E were decreased in eyes with PEX. Beta-crystalline B1 and CRYBB2 and gamma-crystalline D were up to eightfold upregulated in 4 of 10 in eyes with PEX. CONCLUSION: The results indicate that oxidative stress and inflammation are contributing factors in the formation of PEX. Knowledge about the proteome in PEX is relevant for understanding this condition. BMJ Publishing Group 2019-08 2019-03-06 /pmc/articles/PMC6678048/ /pubmed/30842085 http://dx.doi.org/10.1136/bjophthalmol-2017-310416 Text en © Author(s) (or their employer(s)) 2019. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ. This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/. |
spellingShingle | Laboratory Science Botling Taube, Amelie Konzer, Anne Alm, Albert Bergquist, Jonas Proteomic analysis of the aqueous humour in eyes with pseudoexfoliation syndrome |
title | Proteomic analysis of the aqueous humour in eyes with pseudoexfoliation syndrome |
title_full | Proteomic analysis of the aqueous humour in eyes with pseudoexfoliation syndrome |
title_fullStr | Proteomic analysis of the aqueous humour in eyes with pseudoexfoliation syndrome |
title_full_unstemmed | Proteomic analysis of the aqueous humour in eyes with pseudoexfoliation syndrome |
title_short | Proteomic analysis of the aqueous humour in eyes with pseudoexfoliation syndrome |
title_sort | proteomic analysis of the aqueous humour in eyes with pseudoexfoliation syndrome |
topic | Laboratory Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6678048/ https://www.ncbi.nlm.nih.gov/pubmed/30842085 http://dx.doi.org/10.1136/bjophthalmol-2017-310416 |
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