Cargando…
Phenotypic and Genotypic Characterization of Escherichia coli Causing Urinary Tract Infections in Kidney-Transplanted Patients
Urinary tract infection (UTI), frequently caused by uropathogenic Escherichia coli (UPEC), is the most common infection after kidney transplantation (KTx). Untreated, it can lead to urosepsis and impairment of the graft function. We questioned whether the UPEC isolated from KTx patients differed fro...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6678207/ https://www.ncbi.nlm.nih.gov/pubmed/31284699 http://dx.doi.org/10.3390/jcm8070988 |
_version_ | 1783441045956067328 |
---|---|
author | Abo Basha, Jonas Kiel, Matthias Görlich, Dennis Schütte-Nütgen, Katharina Witten, Anika Pavenstädt, Hermann Kahl, Barbara C. Dobrindt, Ulrich Reuter, Stefan |
author_facet | Abo Basha, Jonas Kiel, Matthias Görlich, Dennis Schütte-Nütgen, Katharina Witten, Anika Pavenstädt, Hermann Kahl, Barbara C. Dobrindt, Ulrich Reuter, Stefan |
author_sort | Abo Basha, Jonas |
collection | PubMed |
description | Urinary tract infection (UTI), frequently caused by uropathogenic Escherichia coli (UPEC), is the most common infection after kidney transplantation (KTx). Untreated, it can lead to urosepsis and impairment of the graft function. We questioned whether the UPEC isolated from KTx patients differed from the UPEC of non-KTx patients. Therefore, we determined the genome sequences of 182 UPEC isolates from KTx and control patients in a large German university clinic and pheno- and genotypically compared these two isolated groups. Resistance to the β-lactams, trimethoprim or trimethoprim/sulfamethoxazole was significantly higher among UPEC from KTx than from control patients, whereas both the isolated groups were highly susceptible to fosfomycin. Accordingly, the gene content conferring resistance to β-lactams or trimethoprim, but also to aminoglycosides, was significantly higher in KTx than in control UPEC isolates. E. coli isolates from KTx patients more frequently presented with uncommon UPEC phylogroups expressing higher numbers of plasmid replicons, but interestingly, less UPEC virulence-associated genes than the control group. We conclude that there is no defining subset of virulence traits for UPEC from KTx patients. The clinical history and immunocompromised status of KTx patients enables E. coli strains with low uropathogenic potential, but with increased antibiotic resistance to cause UTIs. |
format | Online Article Text |
id | pubmed-6678207 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-66782072019-08-19 Phenotypic and Genotypic Characterization of Escherichia coli Causing Urinary Tract Infections in Kidney-Transplanted Patients Abo Basha, Jonas Kiel, Matthias Görlich, Dennis Schütte-Nütgen, Katharina Witten, Anika Pavenstädt, Hermann Kahl, Barbara C. Dobrindt, Ulrich Reuter, Stefan J Clin Med Article Urinary tract infection (UTI), frequently caused by uropathogenic Escherichia coli (UPEC), is the most common infection after kidney transplantation (KTx). Untreated, it can lead to urosepsis and impairment of the graft function. We questioned whether the UPEC isolated from KTx patients differed from the UPEC of non-KTx patients. Therefore, we determined the genome sequences of 182 UPEC isolates from KTx and control patients in a large German university clinic and pheno- and genotypically compared these two isolated groups. Resistance to the β-lactams, trimethoprim or trimethoprim/sulfamethoxazole was significantly higher among UPEC from KTx than from control patients, whereas both the isolated groups were highly susceptible to fosfomycin. Accordingly, the gene content conferring resistance to β-lactams or trimethoprim, but also to aminoglycosides, was significantly higher in KTx than in control UPEC isolates. E. coli isolates from KTx patients more frequently presented with uncommon UPEC phylogroups expressing higher numbers of plasmid replicons, but interestingly, less UPEC virulence-associated genes than the control group. We conclude that there is no defining subset of virulence traits for UPEC from KTx patients. The clinical history and immunocompromised status of KTx patients enables E. coli strains with low uropathogenic potential, but with increased antibiotic resistance to cause UTIs. MDPI 2019-07-07 /pmc/articles/PMC6678207/ /pubmed/31284699 http://dx.doi.org/10.3390/jcm8070988 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Abo Basha, Jonas Kiel, Matthias Görlich, Dennis Schütte-Nütgen, Katharina Witten, Anika Pavenstädt, Hermann Kahl, Barbara C. Dobrindt, Ulrich Reuter, Stefan Phenotypic and Genotypic Characterization of Escherichia coli Causing Urinary Tract Infections in Kidney-Transplanted Patients |
title | Phenotypic and Genotypic Characterization of Escherichia coli Causing Urinary Tract Infections in Kidney-Transplanted Patients |
title_full | Phenotypic and Genotypic Characterization of Escherichia coli Causing Urinary Tract Infections in Kidney-Transplanted Patients |
title_fullStr | Phenotypic and Genotypic Characterization of Escherichia coli Causing Urinary Tract Infections in Kidney-Transplanted Patients |
title_full_unstemmed | Phenotypic and Genotypic Characterization of Escherichia coli Causing Urinary Tract Infections in Kidney-Transplanted Patients |
title_short | Phenotypic and Genotypic Characterization of Escherichia coli Causing Urinary Tract Infections in Kidney-Transplanted Patients |
title_sort | phenotypic and genotypic characterization of escherichia coli causing urinary tract infections in kidney-transplanted patients |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6678207/ https://www.ncbi.nlm.nih.gov/pubmed/31284699 http://dx.doi.org/10.3390/jcm8070988 |
work_keys_str_mv | AT abobashajonas phenotypicandgenotypiccharacterizationofescherichiacolicausingurinarytractinfectionsinkidneytransplantedpatients AT kielmatthias phenotypicandgenotypiccharacterizationofescherichiacolicausingurinarytractinfectionsinkidneytransplantedpatients AT gorlichdennis phenotypicandgenotypiccharacterizationofescherichiacolicausingurinarytractinfectionsinkidneytransplantedpatients AT schuttenutgenkatharina phenotypicandgenotypiccharacterizationofescherichiacolicausingurinarytractinfectionsinkidneytransplantedpatients AT wittenanika phenotypicandgenotypiccharacterizationofescherichiacolicausingurinarytractinfectionsinkidneytransplantedpatients AT pavenstadthermann phenotypicandgenotypiccharacterizationofescherichiacolicausingurinarytractinfectionsinkidneytransplantedpatients AT kahlbarbarac phenotypicandgenotypiccharacterizationofescherichiacolicausingurinarytractinfectionsinkidneytransplantedpatients AT dobrindtulrich phenotypicandgenotypiccharacterizationofescherichiacolicausingurinarytractinfectionsinkidneytransplantedpatients AT reuterstefan phenotypicandgenotypiccharacterizationofescherichiacolicausingurinarytractinfectionsinkidneytransplantedpatients |