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The Genome of the Steller Sea Lion (Eumetopias jubatus)

The Steller sea lion is the largest member of the Otariidae family and is found in the coastal waters of the northern Pacific Rim. Here, we present the Steller sea lion genome, determined through DNA sequencing approaches that utilized microfluidic partitioning library construction, as well as nanop...

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Autores principales: Kwan, Harwood H., Culibrk, Luka, Taylor, Gregory A., Leelakumari, Sreeja, Tan, Ryan, Jackman, Shaun D., Tse, Kane, MacLeod, Tina, Cheng, Dean, Chuah, Eric, Kirk, Heather, Pandoh, Pawan, Carlsen, Rebecca, Zhao, Yongjun, Mungall, Andrew J., Moore, Richard, Birol, Inanc, Marra, Marco A., Rosen, David A.S., Haulena, Martin, Jones, Steven J. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6678222/
https://www.ncbi.nlm.nih.gov/pubmed/31248052
http://dx.doi.org/10.3390/genes10070486
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author Kwan, Harwood H.
Culibrk, Luka
Taylor, Gregory A.
Leelakumari, Sreeja
Tan, Ryan
Jackman, Shaun D.
Tse, Kane
MacLeod, Tina
Cheng, Dean
Chuah, Eric
Kirk, Heather
Pandoh, Pawan
Carlsen, Rebecca
Zhao, Yongjun
Mungall, Andrew J.
Moore, Richard
Birol, Inanc
Marra, Marco A.
Rosen, David A.S.
Haulena, Martin
Jones, Steven J. M.
author_facet Kwan, Harwood H.
Culibrk, Luka
Taylor, Gregory A.
Leelakumari, Sreeja
Tan, Ryan
Jackman, Shaun D.
Tse, Kane
MacLeod, Tina
Cheng, Dean
Chuah, Eric
Kirk, Heather
Pandoh, Pawan
Carlsen, Rebecca
Zhao, Yongjun
Mungall, Andrew J.
Moore, Richard
Birol, Inanc
Marra, Marco A.
Rosen, David A.S.
Haulena, Martin
Jones, Steven J. M.
author_sort Kwan, Harwood H.
collection PubMed
description The Steller sea lion is the largest member of the Otariidae family and is found in the coastal waters of the northern Pacific Rim. Here, we present the Steller sea lion genome, determined through DNA sequencing approaches that utilized microfluidic partitioning library construction, as well as nanopore technologies. These methods constructed a highly contiguous assembly with a scaffold N50 length of over 14 megabases, a contig N50 length of over 242 kilobases and a total length of 2.404 gigabases. As a measure of completeness, 95.1% of 4104 highly conserved mammalian genes were found to be complete within the assembly. Further annotation identified 19,668 protein coding genes. The assembled genome sequence and underlying sequence data can be found at the National Center for Biotechnology Information (NCBI) under the BioProject accession number PRJNA475770.
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spelling pubmed-66782222019-08-19 The Genome of the Steller Sea Lion (Eumetopias jubatus) Kwan, Harwood H. Culibrk, Luka Taylor, Gregory A. Leelakumari, Sreeja Tan, Ryan Jackman, Shaun D. Tse, Kane MacLeod, Tina Cheng, Dean Chuah, Eric Kirk, Heather Pandoh, Pawan Carlsen, Rebecca Zhao, Yongjun Mungall, Andrew J. Moore, Richard Birol, Inanc Marra, Marco A. Rosen, David A.S. Haulena, Martin Jones, Steven J. M. Genes (Basel) Article The Steller sea lion is the largest member of the Otariidae family and is found in the coastal waters of the northern Pacific Rim. Here, we present the Steller sea lion genome, determined through DNA sequencing approaches that utilized microfluidic partitioning library construction, as well as nanopore technologies. These methods constructed a highly contiguous assembly with a scaffold N50 length of over 14 megabases, a contig N50 length of over 242 kilobases and a total length of 2.404 gigabases. As a measure of completeness, 95.1% of 4104 highly conserved mammalian genes were found to be complete within the assembly. Further annotation identified 19,668 protein coding genes. The assembled genome sequence and underlying sequence data can be found at the National Center for Biotechnology Information (NCBI) under the BioProject accession number PRJNA475770. MDPI 2019-06-26 /pmc/articles/PMC6678222/ /pubmed/31248052 http://dx.doi.org/10.3390/genes10070486 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kwan, Harwood H.
Culibrk, Luka
Taylor, Gregory A.
Leelakumari, Sreeja
Tan, Ryan
Jackman, Shaun D.
Tse, Kane
MacLeod, Tina
Cheng, Dean
Chuah, Eric
Kirk, Heather
Pandoh, Pawan
Carlsen, Rebecca
Zhao, Yongjun
Mungall, Andrew J.
Moore, Richard
Birol, Inanc
Marra, Marco A.
Rosen, David A.S.
Haulena, Martin
Jones, Steven J. M.
The Genome of the Steller Sea Lion (Eumetopias jubatus)
title The Genome of the Steller Sea Lion (Eumetopias jubatus)
title_full The Genome of the Steller Sea Lion (Eumetopias jubatus)
title_fullStr The Genome of the Steller Sea Lion (Eumetopias jubatus)
title_full_unstemmed The Genome of the Steller Sea Lion (Eumetopias jubatus)
title_short The Genome of the Steller Sea Lion (Eumetopias jubatus)
title_sort genome of the steller sea lion (eumetopias jubatus)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6678222/
https://www.ncbi.nlm.nih.gov/pubmed/31248052
http://dx.doi.org/10.3390/genes10070486
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