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The Caribou (Rangifer tarandus) Genome
Rangifer tarandus, known as caribou or reindeer, is a widespread circumpolar species which presents significant variability in their morphology, ecology, and genetics. A genome was sequenced from a male boreal caribou (R. t. caribou) from Manitoba, Canada. Both paired end and Chicago libraries were...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6678279/ https://www.ncbi.nlm.nih.gov/pubmed/31319535 http://dx.doi.org/10.3390/genes10070540 |
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author | Taylor, Rebecca S. Horn, Rebekah L. Zhang, Xi Golding, G. Brian Manseau, Micheline Wilson, Paul J. |
author_facet | Taylor, Rebecca S. Horn, Rebekah L. Zhang, Xi Golding, G. Brian Manseau, Micheline Wilson, Paul J. |
author_sort | Taylor, Rebecca S. |
collection | PubMed |
description | Rangifer tarandus, known as caribou or reindeer, is a widespread circumpolar species which presents significant variability in their morphology, ecology, and genetics. A genome was sequenced from a male boreal caribou (R. t. caribou) from Manitoba, Canada. Both paired end and Chicago libraries were constructed and sequenced on Illumina platforms. The final assembly consists of approximately 2.205 Gb, and has a scaffold N50 of 11.765 Mb. BUSCO (Benchmarking Universal Single-Copy Orthologs) reconstructed 3820 (93.1%) complete mammalian genes, and genome annotation identified the locations of 33,177 protein-coding genes. An alignment to the bovine genome was carried out, indicating sequence coverage on all bovine chromosomes. A high-quality reference genome will be invaluable for evolutionary research and for conservation efforts for the species. Further information about the genome, including a FASTA file of the assembly and the annotation files, is available on our caribou genome website. Raw sequence data is available at the National Centre for Biotechnology Information (NCBI), under the BioProject accession number PRJNA549927. |
format | Online Article Text |
id | pubmed-6678279 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-66782792019-08-19 The Caribou (Rangifer tarandus) Genome Taylor, Rebecca S. Horn, Rebekah L. Zhang, Xi Golding, G. Brian Manseau, Micheline Wilson, Paul J. Genes (Basel) Article Rangifer tarandus, known as caribou or reindeer, is a widespread circumpolar species which presents significant variability in their morphology, ecology, and genetics. A genome was sequenced from a male boreal caribou (R. t. caribou) from Manitoba, Canada. Both paired end and Chicago libraries were constructed and sequenced on Illumina platforms. The final assembly consists of approximately 2.205 Gb, and has a scaffold N50 of 11.765 Mb. BUSCO (Benchmarking Universal Single-Copy Orthologs) reconstructed 3820 (93.1%) complete mammalian genes, and genome annotation identified the locations of 33,177 protein-coding genes. An alignment to the bovine genome was carried out, indicating sequence coverage on all bovine chromosomes. A high-quality reference genome will be invaluable for evolutionary research and for conservation efforts for the species. Further information about the genome, including a FASTA file of the assembly and the annotation files, is available on our caribou genome website. Raw sequence data is available at the National Centre for Biotechnology Information (NCBI), under the BioProject accession number PRJNA549927. MDPI 2019-07-17 /pmc/articles/PMC6678279/ /pubmed/31319535 http://dx.doi.org/10.3390/genes10070540 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Taylor, Rebecca S. Horn, Rebekah L. Zhang, Xi Golding, G. Brian Manseau, Micheline Wilson, Paul J. The Caribou (Rangifer tarandus) Genome |
title | The Caribou (Rangifer tarandus) Genome |
title_full | The Caribou (Rangifer tarandus) Genome |
title_fullStr | The Caribou (Rangifer tarandus) Genome |
title_full_unstemmed | The Caribou (Rangifer tarandus) Genome |
title_short | The Caribou (Rangifer tarandus) Genome |
title_sort | caribou (rangifer tarandus) genome |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6678279/ https://www.ncbi.nlm.nih.gov/pubmed/31319535 http://dx.doi.org/10.3390/genes10070540 |
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