Cargando…

The Caribou (Rangifer tarandus) Genome

Rangifer tarandus, known as caribou or reindeer, is a widespread circumpolar species which presents significant variability in their morphology, ecology, and genetics. A genome was sequenced from a male boreal caribou (R. t. caribou) from Manitoba, Canada. Both paired end and Chicago libraries were...

Descripción completa

Detalles Bibliográficos
Autores principales: Taylor, Rebecca S., Horn, Rebekah L., Zhang, Xi, Golding, G. Brian, Manseau, Micheline, Wilson, Paul J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6678279/
https://www.ncbi.nlm.nih.gov/pubmed/31319535
http://dx.doi.org/10.3390/genes10070540
_version_ 1783441062877986816
author Taylor, Rebecca S.
Horn, Rebekah L.
Zhang, Xi
Golding, G. Brian
Manseau, Micheline
Wilson, Paul J.
author_facet Taylor, Rebecca S.
Horn, Rebekah L.
Zhang, Xi
Golding, G. Brian
Manseau, Micheline
Wilson, Paul J.
author_sort Taylor, Rebecca S.
collection PubMed
description Rangifer tarandus, known as caribou or reindeer, is a widespread circumpolar species which presents significant variability in their morphology, ecology, and genetics. A genome was sequenced from a male boreal caribou (R. t. caribou) from Manitoba, Canada. Both paired end and Chicago libraries were constructed and sequenced on Illumina platforms. The final assembly consists of approximately 2.205 Gb, and has a scaffold N50 of 11.765 Mb. BUSCO (Benchmarking Universal Single-Copy Orthologs) reconstructed 3820 (93.1%) complete mammalian genes, and genome annotation identified the locations of 33,177 protein-coding genes. An alignment to the bovine genome was carried out, indicating sequence coverage on all bovine chromosomes. A high-quality reference genome will be invaluable for evolutionary research and for conservation efforts for the species. Further information about the genome, including a FASTA file of the assembly and the annotation files, is available on our caribou genome website. Raw sequence data is available at the National Centre for Biotechnology Information (NCBI), under the BioProject accession number PRJNA549927.
format Online
Article
Text
id pubmed-6678279
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-66782792019-08-19 The Caribou (Rangifer tarandus) Genome Taylor, Rebecca S. Horn, Rebekah L. Zhang, Xi Golding, G. Brian Manseau, Micheline Wilson, Paul J. Genes (Basel) Article Rangifer tarandus, known as caribou or reindeer, is a widespread circumpolar species which presents significant variability in their morphology, ecology, and genetics. A genome was sequenced from a male boreal caribou (R. t. caribou) from Manitoba, Canada. Both paired end and Chicago libraries were constructed and sequenced on Illumina platforms. The final assembly consists of approximately 2.205 Gb, and has a scaffold N50 of 11.765 Mb. BUSCO (Benchmarking Universal Single-Copy Orthologs) reconstructed 3820 (93.1%) complete mammalian genes, and genome annotation identified the locations of 33,177 protein-coding genes. An alignment to the bovine genome was carried out, indicating sequence coverage on all bovine chromosomes. A high-quality reference genome will be invaluable for evolutionary research and for conservation efforts for the species. Further information about the genome, including a FASTA file of the assembly and the annotation files, is available on our caribou genome website. Raw sequence data is available at the National Centre for Biotechnology Information (NCBI), under the BioProject accession number PRJNA549927. MDPI 2019-07-17 /pmc/articles/PMC6678279/ /pubmed/31319535 http://dx.doi.org/10.3390/genes10070540 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Taylor, Rebecca S.
Horn, Rebekah L.
Zhang, Xi
Golding, G. Brian
Manseau, Micheline
Wilson, Paul J.
The Caribou (Rangifer tarandus) Genome
title The Caribou (Rangifer tarandus) Genome
title_full The Caribou (Rangifer tarandus) Genome
title_fullStr The Caribou (Rangifer tarandus) Genome
title_full_unstemmed The Caribou (Rangifer tarandus) Genome
title_short The Caribou (Rangifer tarandus) Genome
title_sort caribou (rangifer tarandus) genome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6678279/
https://www.ncbi.nlm.nih.gov/pubmed/31319535
http://dx.doi.org/10.3390/genes10070540
work_keys_str_mv AT taylorrebeccas thecaribourangifertarandusgenome
AT hornrebekahl thecaribourangifertarandusgenome
AT zhangxi thecaribourangifertarandusgenome
AT goldinggbrian thecaribourangifertarandusgenome
AT manseaumicheline thecaribourangifertarandusgenome
AT wilsonpaulj thecaribourangifertarandusgenome
AT taylorrebeccas caribourangifertarandusgenome
AT hornrebekahl caribourangifertarandusgenome
AT zhangxi caribourangifertarandusgenome
AT goldinggbrian caribourangifertarandusgenome
AT manseaumicheline caribourangifertarandusgenome
AT wilsonpaulj caribourangifertarandusgenome