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S. cerevisiae Strain Lacking Mitochondrial IF3 Shows Increased Levels of Tma19p during Adaptation to Respiratory Growth

After billions of years of evolution, mitochondrion retains its own genome, which gets expressed in mitochondrial matrix. Mitochondrial translation machinery rather differs from modern bacterial and eukaryotic cytosolic systems. Any disturbance in mitochondrial translation drastically impairs mitoch...

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Autores principales: Levitskii, Sergey, Baleva, Maria V., Chicherin, Ivan, Krasheninnikov, Igor A., Kamenski, Piotr
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6678281/
https://www.ncbi.nlm.nih.gov/pubmed/31248014
http://dx.doi.org/10.3390/cells8070645
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author Levitskii, Sergey
Baleva, Maria V.
Chicherin, Ivan
Krasheninnikov, Igor A.
Kamenski, Piotr
author_facet Levitskii, Sergey
Baleva, Maria V.
Chicherin, Ivan
Krasheninnikov, Igor A.
Kamenski, Piotr
author_sort Levitskii, Sergey
collection PubMed
description After billions of years of evolution, mitochondrion retains its own genome, which gets expressed in mitochondrial matrix. Mitochondrial translation machinery rather differs from modern bacterial and eukaryotic cytosolic systems. Any disturbance in mitochondrial translation drastically impairs mitochondrial function. In budding yeast Saccharomyces cerevisiae, deletion of the gene coding for mitochondrial translation initiation factor 3—AIM23, leads to an imbalance in mitochondrial protein synthesis and significantly delays growth after shifting from fermentable to non-fermentable carbon sources. Molecular mechanism underlying this adaptation to respiratory growth was unknown. Here, we demonstrate that slow adaptation from glycolysis to respiration in the absence of Aim23p is accompanied by a gradual increase of cytochrome c oxidase activity and by increased levels of Tma19p protein, which protects mitochondria from oxidative stress.
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spelling pubmed-66782812019-08-19 S. cerevisiae Strain Lacking Mitochondrial IF3 Shows Increased Levels of Tma19p during Adaptation to Respiratory Growth Levitskii, Sergey Baleva, Maria V. Chicherin, Ivan Krasheninnikov, Igor A. Kamenski, Piotr Cells Article After billions of years of evolution, mitochondrion retains its own genome, which gets expressed in mitochondrial matrix. Mitochondrial translation machinery rather differs from modern bacterial and eukaryotic cytosolic systems. Any disturbance in mitochondrial translation drastically impairs mitochondrial function. In budding yeast Saccharomyces cerevisiae, deletion of the gene coding for mitochondrial translation initiation factor 3—AIM23, leads to an imbalance in mitochondrial protein synthesis and significantly delays growth after shifting from fermentable to non-fermentable carbon sources. Molecular mechanism underlying this adaptation to respiratory growth was unknown. Here, we demonstrate that slow adaptation from glycolysis to respiration in the absence of Aim23p is accompanied by a gradual increase of cytochrome c oxidase activity and by increased levels of Tma19p protein, which protects mitochondria from oxidative stress. MDPI 2019-06-26 /pmc/articles/PMC6678281/ /pubmed/31248014 http://dx.doi.org/10.3390/cells8070645 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Levitskii, Sergey
Baleva, Maria V.
Chicherin, Ivan
Krasheninnikov, Igor A.
Kamenski, Piotr
S. cerevisiae Strain Lacking Mitochondrial IF3 Shows Increased Levels of Tma19p during Adaptation to Respiratory Growth
title S. cerevisiae Strain Lacking Mitochondrial IF3 Shows Increased Levels of Tma19p during Adaptation to Respiratory Growth
title_full S. cerevisiae Strain Lacking Mitochondrial IF3 Shows Increased Levels of Tma19p during Adaptation to Respiratory Growth
title_fullStr S. cerevisiae Strain Lacking Mitochondrial IF3 Shows Increased Levels of Tma19p during Adaptation to Respiratory Growth
title_full_unstemmed S. cerevisiae Strain Lacking Mitochondrial IF3 Shows Increased Levels of Tma19p during Adaptation to Respiratory Growth
title_short S. cerevisiae Strain Lacking Mitochondrial IF3 Shows Increased Levels of Tma19p during Adaptation to Respiratory Growth
title_sort s. cerevisiae strain lacking mitochondrial if3 shows increased levels of tma19p during adaptation to respiratory growth
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6678281/
https://www.ncbi.nlm.nih.gov/pubmed/31248014
http://dx.doi.org/10.3390/cells8070645
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