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Comparison of the RNA Content of Extracellular Vesicles Derived from Paracoccidioides brasiliensis and Paracoccidioides lutzii
Paracoccidioides brasiliensis and P. lutzii cause human paracoccidioidomycosis. We have previously characterized the <200-nt RNA sub-populations contained in fungal extracellular vesicles (EVs) from P. brasiliensis Pb18 and other pathogenic fungi. We have presently used the RNA-seq strategy to co...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6678485/ https://www.ncbi.nlm.nih.gov/pubmed/31340551 http://dx.doi.org/10.3390/cells8070765 |
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author | Peres da Silva, Roberta Longo, Larissa G. V. da Cunha, Julia P. C. Sobreira, Tiago J. P. Rodrigues, Marcio L. Faoro, Helisson Goldenberg, Samuel Alves, Lysangela R. Puccia, Rosana |
author_facet | Peres da Silva, Roberta Longo, Larissa G. V. da Cunha, Julia P. C. Sobreira, Tiago J. P. Rodrigues, Marcio L. Faoro, Helisson Goldenberg, Samuel Alves, Lysangela R. Puccia, Rosana |
author_sort | Peres da Silva, Roberta |
collection | PubMed |
description | Paracoccidioides brasiliensis and P. lutzii cause human paracoccidioidomycosis. We have previously characterized the <200-nt RNA sub-populations contained in fungal extracellular vesicles (EVs) from P. brasiliensis Pb18 and other pathogenic fungi. We have presently used the RNA-seq strategy to compare the <200- and >200-nt RNA fractions contained in EVs isolated from culture supernatants of P. brasiliensis Pb18, Pb3, and P. lutzii Pb01. Shared mRNA sequences were related to protein modification, translation, and DNA metabolism/biogenesis, while those related to transport and oxidation-reduction were exclusive to Pb01. The presence of functional full-length mRNAs was validated by in vitro translation. Among small non-coding (nc)RNA, 15 were common to all samples; small nucleolar (sno)RNAs were enriched in P. brasiliensis EVs, whereas for P. lutzii there were similar proportions of snoRNA, rRNA, and tRNA. Putative exonic sRNAs were highly abundant in Pb18 EVs. We also found sRNA sequences bearing incomplete microRNA structures mapping to exons. RNA-seq data suggest that extracellular fractions containing Pb18 EVs can modulate the transcriptome of murine monocyte-derived dendritic cells in a transwell system. Considering that sRNA classes are involved in transcription/translation modulation, our general results may indicate that differences in virulence among fungal isolates can be related to their distinct EV-RNA content. |
format | Online Article Text |
id | pubmed-6678485 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-66784852019-08-19 Comparison of the RNA Content of Extracellular Vesicles Derived from Paracoccidioides brasiliensis and Paracoccidioides lutzii Peres da Silva, Roberta Longo, Larissa G. V. da Cunha, Julia P. C. Sobreira, Tiago J. P. Rodrigues, Marcio L. Faoro, Helisson Goldenberg, Samuel Alves, Lysangela R. Puccia, Rosana Cells Article Paracoccidioides brasiliensis and P. lutzii cause human paracoccidioidomycosis. We have previously characterized the <200-nt RNA sub-populations contained in fungal extracellular vesicles (EVs) from P. brasiliensis Pb18 and other pathogenic fungi. We have presently used the RNA-seq strategy to compare the <200- and >200-nt RNA fractions contained in EVs isolated from culture supernatants of P. brasiliensis Pb18, Pb3, and P. lutzii Pb01. Shared mRNA sequences were related to protein modification, translation, and DNA metabolism/biogenesis, while those related to transport and oxidation-reduction were exclusive to Pb01. The presence of functional full-length mRNAs was validated by in vitro translation. Among small non-coding (nc)RNA, 15 were common to all samples; small nucleolar (sno)RNAs were enriched in P. brasiliensis EVs, whereas for P. lutzii there were similar proportions of snoRNA, rRNA, and tRNA. Putative exonic sRNAs were highly abundant in Pb18 EVs. We also found sRNA sequences bearing incomplete microRNA structures mapping to exons. RNA-seq data suggest that extracellular fractions containing Pb18 EVs can modulate the transcriptome of murine monocyte-derived dendritic cells in a transwell system. Considering that sRNA classes are involved in transcription/translation modulation, our general results may indicate that differences in virulence among fungal isolates can be related to their distinct EV-RNA content. MDPI 2019-07-23 /pmc/articles/PMC6678485/ /pubmed/31340551 http://dx.doi.org/10.3390/cells8070765 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Peres da Silva, Roberta Longo, Larissa G. V. da Cunha, Julia P. C. Sobreira, Tiago J. P. Rodrigues, Marcio L. Faoro, Helisson Goldenberg, Samuel Alves, Lysangela R. Puccia, Rosana Comparison of the RNA Content of Extracellular Vesicles Derived from Paracoccidioides brasiliensis and Paracoccidioides lutzii |
title | Comparison of the RNA Content of Extracellular Vesicles Derived from Paracoccidioides
brasiliensis and Paracoccidioides lutzii |
title_full | Comparison of the RNA Content of Extracellular Vesicles Derived from Paracoccidioides
brasiliensis and Paracoccidioides lutzii |
title_fullStr | Comparison of the RNA Content of Extracellular Vesicles Derived from Paracoccidioides
brasiliensis and Paracoccidioides lutzii |
title_full_unstemmed | Comparison of the RNA Content of Extracellular Vesicles Derived from Paracoccidioides
brasiliensis and Paracoccidioides lutzii |
title_short | Comparison of the RNA Content of Extracellular Vesicles Derived from Paracoccidioides
brasiliensis and Paracoccidioides lutzii |
title_sort | comparison of the rna content of extracellular vesicles derived from paracoccidioides
brasiliensis and paracoccidioides lutzii |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6678485/ https://www.ncbi.nlm.nih.gov/pubmed/31340551 http://dx.doi.org/10.3390/cells8070765 |
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