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The Effect of the Repression of Oxidative Stress on Tenocyte Differentiation: A Preliminary Study of a Rat Cell Model Using a Novel Differential Tensile Strain Bioreactor

Because of limitations in the current understanding of the exact pathogenesis of tendinopathy, and the lack of an optimal experimental model, effective therapy for the disease is currently unavailable. This study aims to prove that repression of oxidative stress modulates the differentiation of tend...

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Autores principales: Hsiao, Ming-Yen, Lin, Ping-Cheng, Liao, Wei-Hao, Chen, Wen-Shiang, Hsu, Chia-Hsien, He, Cheng-Kun, Wu, Ya-Wen, Gefen, Amit, Iafisco, Michele, Liu, Lixin, Lin, Feng-Huei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6678503/
https://www.ncbi.nlm.nih.gov/pubmed/31336919
http://dx.doi.org/10.3390/ijms20143437
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author Hsiao, Ming-Yen
Lin, Ping-Cheng
Liao, Wei-Hao
Chen, Wen-Shiang
Hsu, Chia-Hsien
He, Cheng-Kun
Wu, Ya-Wen
Gefen, Amit
Iafisco, Michele
Liu, Lixin
Lin, Feng-Huei
author_facet Hsiao, Ming-Yen
Lin, Ping-Cheng
Liao, Wei-Hao
Chen, Wen-Shiang
Hsu, Chia-Hsien
He, Cheng-Kun
Wu, Ya-Wen
Gefen, Amit
Iafisco, Michele
Liu, Lixin
Lin, Feng-Huei
author_sort Hsiao, Ming-Yen
collection PubMed
description Because of limitations in the current understanding of the exact pathogenesis of tendinopathy, and the lack of an optimal experimental model, effective therapy for the disease is currently unavailable. This study aims to prove that repression of oxidative stress modulates the differentiation of tendon-derived cells (TDCs) sustaining excessive tensile strains, and proposes a novel bioreactor capable of applying differential tensile strains to cultured cells simultaneously. TDCs, including tendon-derived stem cells, tenoblasts, tenocytes, and fibroblasts, were isolated from the patellar tendons of Sprague‒Dawley rats. Cyclic uniaxial stretching with 4% or 8% strain at 0.5 Hz for 8 h was applied to TDCs. TDCs subjected to 8% strain were treated with epigallocatechin gallate (EGCG), piracetam, or no medication. Genes representing non-tenocyte lineage (Pparg, Sox9, and Runx2) and type I and type III collagen were analyzed by quantitative polymerase chain reaction. The 8% strain group showed increased expression of non-tenocyte lineage genes and type III/type I collagen ratios compared with the control and 4% strain groups, and the increased expression was ameliorated with addition of EGCG and piracetam. The model developed in this work could be applied to future research on the pathophysiology of tendinopathy and development of treatment options for the disease. Repression of oxidative stress diminishes the expression of genes indicating aberrant differentiation in a rat cell model, which indicates potential therapeutic intervention of tendinopathy, the often relentlessly degenerate condition.
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spelling pubmed-66785032019-08-19 The Effect of the Repression of Oxidative Stress on Tenocyte Differentiation: A Preliminary Study of a Rat Cell Model Using a Novel Differential Tensile Strain Bioreactor Hsiao, Ming-Yen Lin, Ping-Cheng Liao, Wei-Hao Chen, Wen-Shiang Hsu, Chia-Hsien He, Cheng-Kun Wu, Ya-Wen Gefen, Amit Iafisco, Michele Liu, Lixin Lin, Feng-Huei Int J Mol Sci Article Because of limitations in the current understanding of the exact pathogenesis of tendinopathy, and the lack of an optimal experimental model, effective therapy for the disease is currently unavailable. This study aims to prove that repression of oxidative stress modulates the differentiation of tendon-derived cells (TDCs) sustaining excessive tensile strains, and proposes a novel bioreactor capable of applying differential tensile strains to cultured cells simultaneously. TDCs, including tendon-derived stem cells, tenoblasts, tenocytes, and fibroblasts, were isolated from the patellar tendons of Sprague‒Dawley rats. Cyclic uniaxial stretching with 4% or 8% strain at 0.5 Hz for 8 h was applied to TDCs. TDCs subjected to 8% strain were treated with epigallocatechin gallate (EGCG), piracetam, or no medication. Genes representing non-tenocyte lineage (Pparg, Sox9, and Runx2) and type I and type III collagen were analyzed by quantitative polymerase chain reaction. The 8% strain group showed increased expression of non-tenocyte lineage genes and type III/type I collagen ratios compared with the control and 4% strain groups, and the increased expression was ameliorated with addition of EGCG and piracetam. The model developed in this work could be applied to future research on the pathophysiology of tendinopathy and development of treatment options for the disease. Repression of oxidative stress diminishes the expression of genes indicating aberrant differentiation in a rat cell model, which indicates potential therapeutic intervention of tendinopathy, the often relentlessly degenerate condition. MDPI 2019-07-12 /pmc/articles/PMC6678503/ /pubmed/31336919 http://dx.doi.org/10.3390/ijms20143437 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hsiao, Ming-Yen
Lin, Ping-Cheng
Liao, Wei-Hao
Chen, Wen-Shiang
Hsu, Chia-Hsien
He, Cheng-Kun
Wu, Ya-Wen
Gefen, Amit
Iafisco, Michele
Liu, Lixin
Lin, Feng-Huei
The Effect of the Repression of Oxidative Stress on Tenocyte Differentiation: A Preliminary Study of a Rat Cell Model Using a Novel Differential Tensile Strain Bioreactor
title The Effect of the Repression of Oxidative Stress on Tenocyte Differentiation: A Preliminary Study of a Rat Cell Model Using a Novel Differential Tensile Strain Bioreactor
title_full The Effect of the Repression of Oxidative Stress on Tenocyte Differentiation: A Preliminary Study of a Rat Cell Model Using a Novel Differential Tensile Strain Bioreactor
title_fullStr The Effect of the Repression of Oxidative Stress on Tenocyte Differentiation: A Preliminary Study of a Rat Cell Model Using a Novel Differential Tensile Strain Bioreactor
title_full_unstemmed The Effect of the Repression of Oxidative Stress on Tenocyte Differentiation: A Preliminary Study of a Rat Cell Model Using a Novel Differential Tensile Strain Bioreactor
title_short The Effect of the Repression of Oxidative Stress on Tenocyte Differentiation: A Preliminary Study of a Rat Cell Model Using a Novel Differential Tensile Strain Bioreactor
title_sort effect of the repression of oxidative stress on tenocyte differentiation: a preliminary study of a rat cell model using a novel differential tensile strain bioreactor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6678503/
https://www.ncbi.nlm.nih.gov/pubmed/31336919
http://dx.doi.org/10.3390/ijms20143437
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