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Genetic Analyses of Tanzanian Local Chicken Ecotypes Challenged with Newcastle Disease Virus

Newcastle Disease (ND) is a continuing global threat to domestic poultry, especially in developing countries, where severe outbreaks of velogenic ND virus (NDV) often cause major economic losses to households. Local chickens are of great importance to rural family livelihoods through provision of hi...

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Autores principales: Walugembe, Muhammed, Mushi, James R., Amuzu-Aweh, Esinam N., Chiwanga, Gaspar H., Msoffe, Peter L., Wang, Ying, Saelao, Perot, Kelly, Terra, Gallardo, Rodrigo A., Zhou, Huaijun, Lamont, Susan J., Muhairwa, Amandus P., Dekkers, Jack C.M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6678660/
https://www.ncbi.nlm.nih.gov/pubmed/31319636
http://dx.doi.org/10.3390/genes10070546
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author Walugembe, Muhammed
Mushi, James R.
Amuzu-Aweh, Esinam N.
Chiwanga, Gaspar H.
Msoffe, Peter L.
Wang, Ying
Saelao, Perot
Kelly, Terra
Gallardo, Rodrigo A.
Zhou, Huaijun
Lamont, Susan J.
Muhairwa, Amandus P.
Dekkers, Jack C.M.
author_facet Walugembe, Muhammed
Mushi, James R.
Amuzu-Aweh, Esinam N.
Chiwanga, Gaspar H.
Msoffe, Peter L.
Wang, Ying
Saelao, Perot
Kelly, Terra
Gallardo, Rodrigo A.
Zhou, Huaijun
Lamont, Susan J.
Muhairwa, Amandus P.
Dekkers, Jack C.M.
author_sort Walugembe, Muhammed
collection PubMed
description Newcastle Disease (ND) is a continuing global threat to domestic poultry, especially in developing countries, where severe outbreaks of velogenic ND virus (NDV) often cause major economic losses to households. Local chickens are of great importance to rural family livelihoods through provision of high-quality protein. To investigate the genetic basis of host response to NDV, three popular Tanzanian chicken ecotypes (regional populations) were challenged with a lentogenic (vaccine) strain of NDV at 28 days of age. Various host response phenotypes, including anti-NDV antibody levels (pre-infection and 10 days post-infection, dpi), and viral load (2 and 6 dpi) were measured, in addition to growth rate. We estimated genetic parameters and conducted genome-wide association study analyses by genotyping 1399 chickens using the Affymetrix 600K chicken SNP chip. Estimates of heritability of the evaluated traits were moderate (0.18–0.35). Five quantitative trait loci (QTL) associated with growth and/or response to NDV were identified by single-SNP analyses, with some regions explaining ≥1% of genetic variance based on the Bayes-B method. Immune related genes, such as ETS1, TIRAP, and KIRREL3, were located in regions associated with viral load at 6 dpi. The moderate estimates of heritability and identified QTL indicate that NDV response traits may be improved through selective breeding of chickens to enhance increased NDV resistance and vaccine efficacy in Tanzanian local ecotypes.
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spelling pubmed-66786602019-08-19 Genetic Analyses of Tanzanian Local Chicken Ecotypes Challenged with Newcastle Disease Virus Walugembe, Muhammed Mushi, James R. Amuzu-Aweh, Esinam N. Chiwanga, Gaspar H. Msoffe, Peter L. Wang, Ying Saelao, Perot Kelly, Terra Gallardo, Rodrigo A. Zhou, Huaijun Lamont, Susan J. Muhairwa, Amandus P. Dekkers, Jack C.M. Genes (Basel) Article Newcastle Disease (ND) is a continuing global threat to domestic poultry, especially in developing countries, where severe outbreaks of velogenic ND virus (NDV) often cause major economic losses to households. Local chickens are of great importance to rural family livelihoods through provision of high-quality protein. To investigate the genetic basis of host response to NDV, three popular Tanzanian chicken ecotypes (regional populations) were challenged with a lentogenic (vaccine) strain of NDV at 28 days of age. Various host response phenotypes, including anti-NDV antibody levels (pre-infection and 10 days post-infection, dpi), and viral load (2 and 6 dpi) were measured, in addition to growth rate. We estimated genetic parameters and conducted genome-wide association study analyses by genotyping 1399 chickens using the Affymetrix 600K chicken SNP chip. Estimates of heritability of the evaluated traits were moderate (0.18–0.35). Five quantitative trait loci (QTL) associated with growth and/or response to NDV were identified by single-SNP analyses, with some regions explaining ≥1% of genetic variance based on the Bayes-B method. Immune related genes, such as ETS1, TIRAP, and KIRREL3, were located in regions associated with viral load at 6 dpi. The moderate estimates of heritability and identified QTL indicate that NDV response traits may be improved through selective breeding of chickens to enhance increased NDV resistance and vaccine efficacy in Tanzanian local ecotypes. MDPI 2019-07-17 /pmc/articles/PMC6678660/ /pubmed/31319636 http://dx.doi.org/10.3390/genes10070546 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Walugembe, Muhammed
Mushi, James R.
Amuzu-Aweh, Esinam N.
Chiwanga, Gaspar H.
Msoffe, Peter L.
Wang, Ying
Saelao, Perot
Kelly, Terra
Gallardo, Rodrigo A.
Zhou, Huaijun
Lamont, Susan J.
Muhairwa, Amandus P.
Dekkers, Jack C.M.
Genetic Analyses of Tanzanian Local Chicken Ecotypes Challenged with Newcastle Disease Virus
title Genetic Analyses of Tanzanian Local Chicken Ecotypes Challenged with Newcastle Disease Virus
title_full Genetic Analyses of Tanzanian Local Chicken Ecotypes Challenged with Newcastle Disease Virus
title_fullStr Genetic Analyses of Tanzanian Local Chicken Ecotypes Challenged with Newcastle Disease Virus
title_full_unstemmed Genetic Analyses of Tanzanian Local Chicken Ecotypes Challenged with Newcastle Disease Virus
title_short Genetic Analyses of Tanzanian Local Chicken Ecotypes Challenged with Newcastle Disease Virus
title_sort genetic analyses of tanzanian local chicken ecotypes challenged with newcastle disease virus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6678660/
https://www.ncbi.nlm.nih.gov/pubmed/31319636
http://dx.doi.org/10.3390/genes10070546
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