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An Ex-Vivo Culture System of Ovarian Cancer Faithfully Recapitulating the Pathological Features of Primary Tumors

The success rate of establishing human cancer cell lines is not satisfactory and the established cell lines often do not preserve the molecular and histological features of the original tissues. In this study, we developed a novel culture method which can support proliferation of almost all primary...

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Autores principales: Ghani, Farhana Ishrat, Dendo, Kasumi, Watanabe, Reiko, Yamada, Kenji, Yoshimatsu, Yuki, Yugawa, Takashi, Nakahara, Tomomi, Tanaka, Katsuyuki, Yoshida, Hiroshi, Yoshida, Masayuki, Ishikawa, Mitsuya, Goshima, Naoki, Kato, Tomoyasu, Kiyono, Tohru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6678777/
https://www.ncbi.nlm.nih.gov/pubmed/31248002
http://dx.doi.org/10.3390/cells8070644
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author Ghani, Farhana Ishrat
Dendo, Kasumi
Watanabe, Reiko
Yamada, Kenji
Yoshimatsu, Yuki
Yugawa, Takashi
Nakahara, Tomomi
Tanaka, Katsuyuki
Yoshida, Hiroshi
Yoshida, Masayuki
Ishikawa, Mitsuya
Goshima, Naoki
Kato, Tomoyasu
Kiyono, Tohru
author_facet Ghani, Farhana Ishrat
Dendo, Kasumi
Watanabe, Reiko
Yamada, Kenji
Yoshimatsu, Yuki
Yugawa, Takashi
Nakahara, Tomomi
Tanaka, Katsuyuki
Yoshida, Hiroshi
Yoshida, Masayuki
Ishikawa, Mitsuya
Goshima, Naoki
Kato, Tomoyasu
Kiyono, Tohru
author_sort Ghani, Farhana Ishrat
collection PubMed
description The success rate of establishing human cancer cell lines is not satisfactory and the established cell lines often do not preserve the molecular and histological features of the original tissues. In this study, we developed a novel culture method which can support proliferation of almost all primary epithelial ovarian cancer cells, as well as primary normal human oviductal epithelial cells. Cancer cells from fresh or frozen specimens were enriched by the anti-EpCAM antibody-conjugated magnetic beads, plated on Matrigel-coated plate and cultivated under the optimized culture conditions. Seventeen newly established ovarian cancer cell lines, which included all four major histotypes of ovarian cancer, were confirmed to express histotype-specific markers in vitro. Some of the cell lines from all the four histotypes, except mucinous type, generated tumors in immune-deficient mice and the xenograft tumor tissues recapitulated the corresponding original tissues faithfully. Furthermore, with poorly tumorigenic cell lines including mucinous type, we developed a novel xenograft model which could reconstruct the original tissue architecture through forced expression of a set of oncogenes followed by its silencing. With combination of the novel culture method and cell-derived xenograft system, virtually every epithelial ovarian cancer can be reconstituted in mice in a timely fashion.
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spelling pubmed-66787772019-08-19 An Ex-Vivo Culture System of Ovarian Cancer Faithfully Recapitulating the Pathological Features of Primary Tumors Ghani, Farhana Ishrat Dendo, Kasumi Watanabe, Reiko Yamada, Kenji Yoshimatsu, Yuki Yugawa, Takashi Nakahara, Tomomi Tanaka, Katsuyuki Yoshida, Hiroshi Yoshida, Masayuki Ishikawa, Mitsuya Goshima, Naoki Kato, Tomoyasu Kiyono, Tohru Cells Article The success rate of establishing human cancer cell lines is not satisfactory and the established cell lines often do not preserve the molecular and histological features of the original tissues. In this study, we developed a novel culture method which can support proliferation of almost all primary epithelial ovarian cancer cells, as well as primary normal human oviductal epithelial cells. Cancer cells from fresh or frozen specimens were enriched by the anti-EpCAM antibody-conjugated magnetic beads, plated on Matrigel-coated plate and cultivated under the optimized culture conditions. Seventeen newly established ovarian cancer cell lines, which included all four major histotypes of ovarian cancer, were confirmed to express histotype-specific markers in vitro. Some of the cell lines from all the four histotypes, except mucinous type, generated tumors in immune-deficient mice and the xenograft tumor tissues recapitulated the corresponding original tissues faithfully. Furthermore, with poorly tumorigenic cell lines including mucinous type, we developed a novel xenograft model which could reconstruct the original tissue architecture through forced expression of a set of oncogenes followed by its silencing. With combination of the novel culture method and cell-derived xenograft system, virtually every epithelial ovarian cancer can be reconstituted in mice in a timely fashion. MDPI 2019-06-26 /pmc/articles/PMC6678777/ /pubmed/31248002 http://dx.doi.org/10.3390/cells8070644 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ghani, Farhana Ishrat
Dendo, Kasumi
Watanabe, Reiko
Yamada, Kenji
Yoshimatsu, Yuki
Yugawa, Takashi
Nakahara, Tomomi
Tanaka, Katsuyuki
Yoshida, Hiroshi
Yoshida, Masayuki
Ishikawa, Mitsuya
Goshima, Naoki
Kato, Tomoyasu
Kiyono, Tohru
An Ex-Vivo Culture System of Ovarian Cancer Faithfully Recapitulating the Pathological Features of Primary Tumors
title An Ex-Vivo Culture System of Ovarian Cancer Faithfully Recapitulating the Pathological Features of Primary Tumors
title_full An Ex-Vivo Culture System of Ovarian Cancer Faithfully Recapitulating the Pathological Features of Primary Tumors
title_fullStr An Ex-Vivo Culture System of Ovarian Cancer Faithfully Recapitulating the Pathological Features of Primary Tumors
title_full_unstemmed An Ex-Vivo Culture System of Ovarian Cancer Faithfully Recapitulating the Pathological Features of Primary Tumors
title_short An Ex-Vivo Culture System of Ovarian Cancer Faithfully Recapitulating the Pathological Features of Primary Tumors
title_sort ex-vivo culture system of ovarian cancer faithfully recapitulating the pathological features of primary tumors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6678777/
https://www.ncbi.nlm.nih.gov/pubmed/31248002
http://dx.doi.org/10.3390/cells8070644
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