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Identification of Jasmonic Acid Biosynthetic Genes in Sweet Cherry and Expression Analysis in Four Ancient Varieties from Tuscany

Sweet cherries are non-climacteric fruits whose early development is characterized by high levels of the phytohormone jasmonic acid (JA). Important parameters, such as firmness and susceptibility to cracking, can be affected by pre- and postharvest treatments of sweet cherries with JA. Despite the i...

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Autores principales: Berni, Roberto, Cai, Giampiero, Xu, Xuan, Hausman, Jean-Francois, Guerriero, Gea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6678830/
https://www.ncbi.nlm.nih.gov/pubmed/31336562
http://dx.doi.org/10.3390/ijms20143569
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author Berni, Roberto
Cai, Giampiero
Xu, Xuan
Hausman, Jean-Francois
Guerriero, Gea
author_facet Berni, Roberto
Cai, Giampiero
Xu, Xuan
Hausman, Jean-Francois
Guerriero, Gea
author_sort Berni, Roberto
collection PubMed
description Sweet cherries are non-climacteric fruits whose early development is characterized by high levels of the phytohormone jasmonic acid (JA). Important parameters, such as firmness and susceptibility to cracking, can be affected by pre- and postharvest treatments of sweet cherries with JA. Despite the impact of JA on sweet cherry development and fruit characteristics, there are no studies (to the best of our knowledge) identifying the genes involved in the JA biosynthetic pathway in this species. We herein identify the sweet cherry members of the lipoxygenase family (13-LOX); allene oxide synthase, allene oxide cyclase and 12-oxo-phytodienoic acid reductase 3, as well as genes encoding the transcriptional master regulator MYC2. We analyze their expression pattern in four non-commercial Tuscan varieties (‘Carlotta’, ‘Maggiola’, ‘Morellona’, ‘Crognola’) having different levels of bioactives (namely phenolics). The highest differences are found in two genes encoding 13-LOX in the variety ‘Maggiola’ and one MYC2 isoform in ‘Morellona’. No statistically-significant variations are instead present in the allene oxide synthase, allene oxide cyclase and 12-oxo-phytodienoic acid reductase 3. Our data pave the way to follow-up studies on the JA signaling pathway in these ancient varieties, for example in relation to development and post-harvest storage.
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spelling pubmed-66788302019-08-19 Identification of Jasmonic Acid Biosynthetic Genes in Sweet Cherry and Expression Analysis in Four Ancient Varieties from Tuscany Berni, Roberto Cai, Giampiero Xu, Xuan Hausman, Jean-Francois Guerriero, Gea Int J Mol Sci Communication Sweet cherries are non-climacteric fruits whose early development is characterized by high levels of the phytohormone jasmonic acid (JA). Important parameters, such as firmness and susceptibility to cracking, can be affected by pre- and postharvest treatments of sweet cherries with JA. Despite the impact of JA on sweet cherry development and fruit characteristics, there are no studies (to the best of our knowledge) identifying the genes involved in the JA biosynthetic pathway in this species. We herein identify the sweet cherry members of the lipoxygenase family (13-LOX); allene oxide synthase, allene oxide cyclase and 12-oxo-phytodienoic acid reductase 3, as well as genes encoding the transcriptional master regulator MYC2. We analyze their expression pattern in four non-commercial Tuscan varieties (‘Carlotta’, ‘Maggiola’, ‘Morellona’, ‘Crognola’) having different levels of bioactives (namely phenolics). The highest differences are found in two genes encoding 13-LOX in the variety ‘Maggiola’ and one MYC2 isoform in ‘Morellona’. No statistically-significant variations are instead present in the allene oxide synthase, allene oxide cyclase and 12-oxo-phytodienoic acid reductase 3. Our data pave the way to follow-up studies on the JA signaling pathway in these ancient varieties, for example in relation to development and post-harvest storage. MDPI 2019-07-22 /pmc/articles/PMC6678830/ /pubmed/31336562 http://dx.doi.org/10.3390/ijms20143569 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Berni, Roberto
Cai, Giampiero
Xu, Xuan
Hausman, Jean-Francois
Guerriero, Gea
Identification of Jasmonic Acid Biosynthetic Genes in Sweet Cherry and Expression Analysis in Four Ancient Varieties from Tuscany
title Identification of Jasmonic Acid Biosynthetic Genes in Sweet Cherry and Expression Analysis in Four Ancient Varieties from Tuscany
title_full Identification of Jasmonic Acid Biosynthetic Genes in Sweet Cherry and Expression Analysis in Four Ancient Varieties from Tuscany
title_fullStr Identification of Jasmonic Acid Biosynthetic Genes in Sweet Cherry and Expression Analysis in Four Ancient Varieties from Tuscany
title_full_unstemmed Identification of Jasmonic Acid Biosynthetic Genes in Sweet Cherry and Expression Analysis in Four Ancient Varieties from Tuscany
title_short Identification of Jasmonic Acid Biosynthetic Genes in Sweet Cherry and Expression Analysis in Four Ancient Varieties from Tuscany
title_sort identification of jasmonic acid biosynthetic genes in sweet cherry and expression analysis in four ancient varieties from tuscany
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6678830/
https://www.ncbi.nlm.nih.gov/pubmed/31336562
http://dx.doi.org/10.3390/ijms20143569
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