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Identification and Characterization of a Thermotolerant TILLING Allele of Heat Shock Binding Protein 1 in Tomato

The identification of heat stress (HS)-resilient germplasm is important to ensure food security under less favorable environmental conditions. For that, germplasm with an altered activity of factors regulating the HS response is an important genetic tool for crop improvement. Heat shock binding prot...

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Autores principales: Marko, Dominik, El-shershaby, Asmaa, Carriero, Filomena, Summerer, Stephan, Petrozza, Angelo, Iannacone, Rina, Schleiff, Enrico, Fragkostefanakis, Sotirios
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6678839/
https://www.ncbi.nlm.nih.gov/pubmed/31284688
http://dx.doi.org/10.3390/genes10070516
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author Marko, Dominik
El-shershaby, Asmaa
Carriero, Filomena
Summerer, Stephan
Petrozza, Angelo
Iannacone, Rina
Schleiff, Enrico
Fragkostefanakis, Sotirios
author_facet Marko, Dominik
El-shershaby, Asmaa
Carriero, Filomena
Summerer, Stephan
Petrozza, Angelo
Iannacone, Rina
Schleiff, Enrico
Fragkostefanakis, Sotirios
author_sort Marko, Dominik
collection PubMed
description The identification of heat stress (HS)-resilient germplasm is important to ensure food security under less favorable environmental conditions. For that, germplasm with an altered activity of factors regulating the HS response is an important genetic tool for crop improvement. Heat shock binding protein (HSBP) is one of the main negative regulators of HS response, acting as a repressor of the activity of HS transcription factors. We identified a TILLING allele of Solanum lycopersicum (tomato) HSBP1. We examined the effects of the mutation on the functionality of the protein in tomato protoplasts, and compared the thermotolerance capacity of lines carrying the wild-type and mutant alleles of HSBP1. The methionine-to-isoleucine mutation in the central heptad repeats of HSBP1 leads to a partial loss of protein function, thereby reducing the inhibitory effect on Hsf activity. Mutant seedlings show enhanced basal thermotolerance, while mature plants exhibit increased resilience in repeated HS treatments, as shown by several physiological parameters. Importantly, plants that are homozygous for the wild-type or mutant HSBP1 alleles showed no significant differences under non-stressed conditions. Altogether, these results indicate that the identified mutant HSBP1 allele can be used as a genetic tool in breeding, aiming to improve the thermotolerance of tomato varieties.
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spelling pubmed-66788392019-08-19 Identification and Characterization of a Thermotolerant TILLING Allele of Heat Shock Binding Protein 1 in Tomato Marko, Dominik El-shershaby, Asmaa Carriero, Filomena Summerer, Stephan Petrozza, Angelo Iannacone, Rina Schleiff, Enrico Fragkostefanakis, Sotirios Genes (Basel) Article The identification of heat stress (HS)-resilient germplasm is important to ensure food security under less favorable environmental conditions. For that, germplasm with an altered activity of factors regulating the HS response is an important genetic tool for crop improvement. Heat shock binding protein (HSBP) is one of the main negative regulators of HS response, acting as a repressor of the activity of HS transcription factors. We identified a TILLING allele of Solanum lycopersicum (tomato) HSBP1. We examined the effects of the mutation on the functionality of the protein in tomato protoplasts, and compared the thermotolerance capacity of lines carrying the wild-type and mutant alleles of HSBP1. The methionine-to-isoleucine mutation in the central heptad repeats of HSBP1 leads to a partial loss of protein function, thereby reducing the inhibitory effect on Hsf activity. Mutant seedlings show enhanced basal thermotolerance, while mature plants exhibit increased resilience in repeated HS treatments, as shown by several physiological parameters. Importantly, plants that are homozygous for the wild-type or mutant HSBP1 alleles showed no significant differences under non-stressed conditions. Altogether, these results indicate that the identified mutant HSBP1 allele can be used as a genetic tool in breeding, aiming to improve the thermotolerance of tomato varieties. MDPI 2019-07-07 /pmc/articles/PMC6678839/ /pubmed/31284688 http://dx.doi.org/10.3390/genes10070516 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Marko, Dominik
El-shershaby, Asmaa
Carriero, Filomena
Summerer, Stephan
Petrozza, Angelo
Iannacone, Rina
Schleiff, Enrico
Fragkostefanakis, Sotirios
Identification and Characterization of a Thermotolerant TILLING Allele of Heat Shock Binding Protein 1 in Tomato
title Identification and Characterization of a Thermotolerant TILLING Allele of Heat Shock Binding Protein 1 in Tomato
title_full Identification and Characterization of a Thermotolerant TILLING Allele of Heat Shock Binding Protein 1 in Tomato
title_fullStr Identification and Characterization of a Thermotolerant TILLING Allele of Heat Shock Binding Protein 1 in Tomato
title_full_unstemmed Identification and Characterization of a Thermotolerant TILLING Allele of Heat Shock Binding Protein 1 in Tomato
title_short Identification and Characterization of a Thermotolerant TILLING Allele of Heat Shock Binding Protein 1 in Tomato
title_sort identification and characterization of a thermotolerant tilling allele of heat shock binding protein 1 in tomato
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6678839/
https://www.ncbi.nlm.nih.gov/pubmed/31284688
http://dx.doi.org/10.3390/genes10070516
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