Cargando…

Hydrogen Sulfide as a Toxic Product in the Small–Large Intestine Axis and its Role in IBD Development

The small–large intestine axis in hydrogen sulfide accumulation and testing of sulfate and lactate in the gut–gut axis of the intestinal environment has not been well described. Sulfate reducing bacteria (SRB) of the Desulfovibrio genus reduce sulfate to hydrogen sulfide and can be involved in ulcer...

Descripción completa

Detalles Bibliográficos
Autores principales: Kushkevych, Ivan, Dordević, Dani, Kollar, Peter, Vítězová, Monika, Drago, Lorenzo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6679076/
https://www.ncbi.nlm.nih.gov/pubmed/31330956
http://dx.doi.org/10.3390/jcm8071054
_version_ 1783441254051217408
author Kushkevych, Ivan
Dordević, Dani
Kollar, Peter
Vítězová, Monika
Drago, Lorenzo
author_facet Kushkevych, Ivan
Dordević, Dani
Kollar, Peter
Vítězová, Monika
Drago, Lorenzo
author_sort Kushkevych, Ivan
collection PubMed
description The small–large intestine axis in hydrogen sulfide accumulation and testing of sulfate and lactate in the gut–gut axis of the intestinal environment has not been well described. Sulfate reducing bacteria (SRB) of the Desulfovibrio genus reduce sulfate to hydrogen sulfide and can be involved in ulcerative colitis development. The background of the research was to find correlations between hydrogen sulfide production under the effect of an electron acceptor (sulfate) and donor (lactate) at different concentrations and Desulfovibrio piger Vib-7 growth, as well as their dissimilatory sulfate reduction in the intestinal small–large intestinal environment. Methods: Microbiological, biochemical, and biophysical methods, and statistical processing of the results (principal component and cross-correlation analyses) were used. Results: D. piger Vib-7 showed increased intensity of bacterial growth and hydrogen sulfide production under the following concentrations of sulfate and lactate: 17.4 mM and 35.6 mM, respectively. The study showed in what kind of intestinal environment D. piger Vib-7 grows at the highest level and produces the highest amount of hydrogen sulfide. Conclusions: The optimum intestinal environment of D. piger Vib-7 can serve as a good indicator of the occurrence of inflammatory bowel diseases; meaning that these findings can be broadly used in medicine practice dealing with the monitoring and diagnosis of intestinal ailments.
format Online
Article
Text
id pubmed-6679076
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-66790762019-08-19 Hydrogen Sulfide as a Toxic Product in the Small–Large Intestine Axis and its Role in IBD Development Kushkevych, Ivan Dordević, Dani Kollar, Peter Vítězová, Monika Drago, Lorenzo J Clin Med Article The small–large intestine axis in hydrogen sulfide accumulation and testing of sulfate and lactate in the gut–gut axis of the intestinal environment has not been well described. Sulfate reducing bacteria (SRB) of the Desulfovibrio genus reduce sulfate to hydrogen sulfide and can be involved in ulcerative colitis development. The background of the research was to find correlations between hydrogen sulfide production under the effect of an electron acceptor (sulfate) and donor (lactate) at different concentrations and Desulfovibrio piger Vib-7 growth, as well as their dissimilatory sulfate reduction in the intestinal small–large intestinal environment. Methods: Microbiological, biochemical, and biophysical methods, and statistical processing of the results (principal component and cross-correlation analyses) were used. Results: D. piger Vib-7 showed increased intensity of bacterial growth and hydrogen sulfide production under the following concentrations of sulfate and lactate: 17.4 mM and 35.6 mM, respectively. The study showed in what kind of intestinal environment D. piger Vib-7 grows at the highest level and produces the highest amount of hydrogen sulfide. Conclusions: The optimum intestinal environment of D. piger Vib-7 can serve as a good indicator of the occurrence of inflammatory bowel diseases; meaning that these findings can be broadly used in medicine practice dealing with the monitoring and diagnosis of intestinal ailments. MDPI 2019-07-19 /pmc/articles/PMC6679076/ /pubmed/31330956 http://dx.doi.org/10.3390/jcm8071054 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kushkevych, Ivan
Dordević, Dani
Kollar, Peter
Vítězová, Monika
Drago, Lorenzo
Hydrogen Sulfide as a Toxic Product in the Small–Large Intestine Axis and its Role in IBD Development
title Hydrogen Sulfide as a Toxic Product in the Small–Large Intestine Axis and its Role in IBD Development
title_full Hydrogen Sulfide as a Toxic Product in the Small–Large Intestine Axis and its Role in IBD Development
title_fullStr Hydrogen Sulfide as a Toxic Product in the Small–Large Intestine Axis and its Role in IBD Development
title_full_unstemmed Hydrogen Sulfide as a Toxic Product in the Small–Large Intestine Axis and its Role in IBD Development
title_short Hydrogen Sulfide as a Toxic Product in the Small–Large Intestine Axis and its Role in IBD Development
title_sort hydrogen sulfide as a toxic product in the small–large intestine axis and its role in ibd development
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6679076/
https://www.ncbi.nlm.nih.gov/pubmed/31330956
http://dx.doi.org/10.3390/jcm8071054
work_keys_str_mv AT kushkevychivan hydrogensulfideasatoxicproductinthesmalllargeintestineaxisanditsroleinibddevelopment
AT dordevicdani hydrogensulfideasatoxicproductinthesmalllargeintestineaxisanditsroleinibddevelopment
AT kollarpeter hydrogensulfideasatoxicproductinthesmalllargeintestineaxisanditsroleinibddevelopment
AT vitezovamonika hydrogensulfideasatoxicproductinthesmalllargeintestineaxisanditsroleinibddevelopment
AT dragolorenzo hydrogensulfideasatoxicproductinthesmalllargeintestineaxisanditsroleinibddevelopment