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Hsa-miR-34a-5p and hsa-miR-375 as Biomarkers for Monitoring the Effects of Drug Treatment for Migraine Pain in Children and Adolescents: A Pilot Study

MicroRNAs (miRs) have emerged as biomarkers of migraine disease in both adults and children. In this study we evaluated the expression of hsa-miR-34a-5p and hsa-miR-375 in serum and saliva of young subjects (age 11 ± 3.467 years) with migraine without aura (MWA), while some underwent pharmacological...

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Autores principales: Gallelli, Luca, Cione, Erika, Peltrone, Fancesco, Siviglia, Serena, Verano, Antonio, Chirchiglia, Domenico, Zampogna, Stefania, Guidetti, Vincenzo, Sammartino, Luca, Montana, Angelo, Caroleo, Maria Cristina, De Sarro, Giovambattista, Di Mizio, Giulio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6679182/
https://www.ncbi.nlm.nih.gov/pubmed/31252698
http://dx.doi.org/10.3390/jcm8070928
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author Gallelli, Luca
Cione, Erika
Peltrone, Fancesco
Siviglia, Serena
Verano, Antonio
Chirchiglia, Domenico
Zampogna, Stefania
Guidetti, Vincenzo
Sammartino, Luca
Montana, Angelo
Caroleo, Maria Cristina
De Sarro, Giovambattista
Di Mizio, Giulio
author_facet Gallelli, Luca
Cione, Erika
Peltrone, Fancesco
Siviglia, Serena
Verano, Antonio
Chirchiglia, Domenico
Zampogna, Stefania
Guidetti, Vincenzo
Sammartino, Luca
Montana, Angelo
Caroleo, Maria Cristina
De Sarro, Giovambattista
Di Mizio, Giulio
author_sort Gallelli, Luca
collection PubMed
description MicroRNAs (miRs) have emerged as biomarkers of migraine disease in both adults and children. In this study we evaluated the expression of hsa-miR-34a-5p and hsa-miR-375 in serum and saliva of young subjects (age 11 ± 3.467 years) with migraine without aura (MWA), while some underwent pharmacological treatment, and healthy young subjects were used as controls. miRs were determined using the qRT-PCR method, and gene targets of hsa-miR-34a-5p and hsa-miR-375 linked to pain-migraine were found by in silico analysis. qRT-PCR revealed comparable levels of hsa-miRs in both blood and saliva. Higher expression of hsa-miR-34a-5p and hsa-miR-375 was detected in saliva of untreated MWAs compared to healthy subjects (hsa-miR-34a-5p: p < 0.05; hsa-miR-375 p < 0.01). Furthermore, in MWA treated subjects, a significant decrease of hsa-miR-34a-5p and of hsa-miR-375 was documented in saliva and blood compared to MWA untreated ones. Altogether, these findings suggested thathsa-miR-34a-5p and hsa-miR-375 are expressed equally in blood and saliva and that they could be a useful biomarker of disease and of drug efficacy in patients with MWA.
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spelling pubmed-66791822019-08-19 Hsa-miR-34a-5p and hsa-miR-375 as Biomarkers for Monitoring the Effects of Drug Treatment for Migraine Pain in Children and Adolescents: A Pilot Study Gallelli, Luca Cione, Erika Peltrone, Fancesco Siviglia, Serena Verano, Antonio Chirchiglia, Domenico Zampogna, Stefania Guidetti, Vincenzo Sammartino, Luca Montana, Angelo Caroleo, Maria Cristina De Sarro, Giovambattista Di Mizio, Giulio J Clin Med Article MicroRNAs (miRs) have emerged as biomarkers of migraine disease in both adults and children. In this study we evaluated the expression of hsa-miR-34a-5p and hsa-miR-375 in serum and saliva of young subjects (age 11 ± 3.467 years) with migraine without aura (MWA), while some underwent pharmacological treatment, and healthy young subjects were used as controls. miRs were determined using the qRT-PCR method, and gene targets of hsa-miR-34a-5p and hsa-miR-375 linked to pain-migraine were found by in silico analysis. qRT-PCR revealed comparable levels of hsa-miRs in both blood and saliva. Higher expression of hsa-miR-34a-5p and hsa-miR-375 was detected in saliva of untreated MWAs compared to healthy subjects (hsa-miR-34a-5p: p < 0.05; hsa-miR-375 p < 0.01). Furthermore, in MWA treated subjects, a significant decrease of hsa-miR-34a-5p and of hsa-miR-375 was documented in saliva and blood compared to MWA untreated ones. Altogether, these findings suggested thathsa-miR-34a-5p and hsa-miR-375 are expressed equally in blood and saliva and that they could be a useful biomarker of disease and of drug efficacy in patients with MWA. MDPI 2019-06-27 /pmc/articles/PMC6679182/ /pubmed/31252698 http://dx.doi.org/10.3390/jcm8070928 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gallelli, Luca
Cione, Erika
Peltrone, Fancesco
Siviglia, Serena
Verano, Antonio
Chirchiglia, Domenico
Zampogna, Stefania
Guidetti, Vincenzo
Sammartino, Luca
Montana, Angelo
Caroleo, Maria Cristina
De Sarro, Giovambattista
Di Mizio, Giulio
Hsa-miR-34a-5p and hsa-miR-375 as Biomarkers for Monitoring the Effects of Drug Treatment for Migraine Pain in Children and Adolescents: A Pilot Study
title Hsa-miR-34a-5p and hsa-miR-375 as Biomarkers for Monitoring the Effects of Drug Treatment for Migraine Pain in Children and Adolescents: A Pilot Study
title_full Hsa-miR-34a-5p and hsa-miR-375 as Biomarkers for Monitoring the Effects of Drug Treatment for Migraine Pain in Children and Adolescents: A Pilot Study
title_fullStr Hsa-miR-34a-5p and hsa-miR-375 as Biomarkers for Monitoring the Effects of Drug Treatment for Migraine Pain in Children and Adolescents: A Pilot Study
title_full_unstemmed Hsa-miR-34a-5p and hsa-miR-375 as Biomarkers for Monitoring the Effects of Drug Treatment for Migraine Pain in Children and Adolescents: A Pilot Study
title_short Hsa-miR-34a-5p and hsa-miR-375 as Biomarkers for Monitoring the Effects of Drug Treatment for Migraine Pain in Children and Adolescents: A Pilot Study
title_sort hsa-mir-34a-5p and hsa-mir-375 as biomarkers for monitoring the effects of drug treatment for migraine pain in children and adolescents: a pilot study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6679182/
https://www.ncbi.nlm.nih.gov/pubmed/31252698
http://dx.doi.org/10.3390/jcm8070928
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