Human Skeletal Muscle Cells Derived from the Orbicularis Oculi Have Regenerative Capacity for Duchenne Muscular Dystrophy

Skeletal muscle stem cells (MuSCs) have been proposed as suitable candidates for cell therapy in muscular disorders since they exhibit good capacity for myogenic regeneration. However, for better therapeutic outcomes, it is necessary to isolate human MuSCs from a suitable tissue source with high myo...

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Autores principales: Yamanaka, Yukito, Takenaka, Nana, Sakurai, Hidetoshi, Ueno, Morio, Kinoshita, Shigeru, Sotozono, Chie, Sato, Takahiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6679213/
https://www.ncbi.nlm.nih.gov/pubmed/31337111
http://dx.doi.org/10.3390/ijms20143456
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author Yamanaka, Yukito
Takenaka, Nana
Sakurai, Hidetoshi
Ueno, Morio
Kinoshita, Shigeru
Sotozono, Chie
Sato, Takahiko
author_facet Yamanaka, Yukito
Takenaka, Nana
Sakurai, Hidetoshi
Ueno, Morio
Kinoshita, Shigeru
Sotozono, Chie
Sato, Takahiko
author_sort Yamanaka, Yukito
collection PubMed
description Skeletal muscle stem cells (MuSCs) have been proposed as suitable candidates for cell therapy in muscular disorders since they exhibit good capacity for myogenic regeneration. However, for better therapeutic outcomes, it is necessary to isolate human MuSCs from a suitable tissue source with high myogenic differentiation. In this context, we isolated CD56+CD82+ cells from the extra eyelid tissue of young and aged patients, and tested in vitro myogenic differentiation potential. In the current study, myogenic cells derived from extra eyelid tissue were characterized and compared with immortalized human myogenic cells. We found that myogenic cells derived from extra eyelid tissue proliferated and differentiated myofibers in vitro, and restored DYSTROPHIN or PAX7 expression after transplantation with these cells in mice with Duchenne muscular dystrophy. Thus, human myogenic cells derived from extra eyelid tissue including the orbicularis oculi might be good candidates for stem cell-based therapies for treating muscular diseases.
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spelling pubmed-66792132019-08-19 Human Skeletal Muscle Cells Derived from the Orbicularis Oculi Have Regenerative Capacity for Duchenne Muscular Dystrophy Yamanaka, Yukito Takenaka, Nana Sakurai, Hidetoshi Ueno, Morio Kinoshita, Shigeru Sotozono, Chie Sato, Takahiko Int J Mol Sci Communication Skeletal muscle stem cells (MuSCs) have been proposed as suitable candidates for cell therapy in muscular disorders since they exhibit good capacity for myogenic regeneration. However, for better therapeutic outcomes, it is necessary to isolate human MuSCs from a suitable tissue source with high myogenic differentiation. In this context, we isolated CD56+CD82+ cells from the extra eyelid tissue of young and aged patients, and tested in vitro myogenic differentiation potential. In the current study, myogenic cells derived from extra eyelid tissue were characterized and compared with immortalized human myogenic cells. We found that myogenic cells derived from extra eyelid tissue proliferated and differentiated myofibers in vitro, and restored DYSTROPHIN or PAX7 expression after transplantation with these cells in mice with Duchenne muscular dystrophy. Thus, human myogenic cells derived from extra eyelid tissue including the orbicularis oculi might be good candidates for stem cell-based therapies for treating muscular diseases. MDPI 2019-07-14 /pmc/articles/PMC6679213/ /pubmed/31337111 http://dx.doi.org/10.3390/ijms20143456 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Yamanaka, Yukito
Takenaka, Nana
Sakurai, Hidetoshi
Ueno, Morio
Kinoshita, Shigeru
Sotozono, Chie
Sato, Takahiko
Human Skeletal Muscle Cells Derived from the Orbicularis Oculi Have Regenerative Capacity for Duchenne Muscular Dystrophy
title Human Skeletal Muscle Cells Derived from the Orbicularis Oculi Have Regenerative Capacity for Duchenne Muscular Dystrophy
title_full Human Skeletal Muscle Cells Derived from the Orbicularis Oculi Have Regenerative Capacity for Duchenne Muscular Dystrophy
title_fullStr Human Skeletal Muscle Cells Derived from the Orbicularis Oculi Have Regenerative Capacity for Duchenne Muscular Dystrophy
title_full_unstemmed Human Skeletal Muscle Cells Derived from the Orbicularis Oculi Have Regenerative Capacity for Duchenne Muscular Dystrophy
title_short Human Skeletal Muscle Cells Derived from the Orbicularis Oculi Have Regenerative Capacity for Duchenne Muscular Dystrophy
title_sort human skeletal muscle cells derived from the orbicularis oculi have regenerative capacity for duchenne muscular dystrophy
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6679213/
https://www.ncbi.nlm.nih.gov/pubmed/31337111
http://dx.doi.org/10.3390/ijms20143456
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