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Redundant SCARECROW genes pattern distinct cell layers in roots and leaves of maize

The highly efficient C(4) photosynthetic pathway is facilitated by ‘Kranz’ leaf anatomy. In Kranz leaves, closely spaced veins are encircled by concentric layers of photosynthetic bundle sheath (inner) and mesophyll (outer) cells. Here, we demonstrate that, in the C(4) monocot maize, Kranz patternin...

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Autores principales: Hughes, Thomas E., Sedelnikova, Olga V., Wu, Hao, Becraft, Philip W., Langdale, Jane A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6679360/
https://www.ncbi.nlm.nih.gov/pubmed/31235633
http://dx.doi.org/10.1242/dev.177543
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author Hughes, Thomas E.
Sedelnikova, Olga V.
Wu, Hao
Becraft, Philip W.
Langdale, Jane A.
author_facet Hughes, Thomas E.
Sedelnikova, Olga V.
Wu, Hao
Becraft, Philip W.
Langdale, Jane A.
author_sort Hughes, Thomas E.
collection PubMed
description The highly efficient C(4) photosynthetic pathway is facilitated by ‘Kranz’ leaf anatomy. In Kranz leaves, closely spaced veins are encircled by concentric layers of photosynthetic bundle sheath (inner) and mesophyll (outer) cells. Here, we demonstrate that, in the C(4) monocot maize, Kranz patterning is regulated by redundant function of SCARECROW 1 (ZmSCR1) and a previously uncharacterized homeologue: ZmSCR1h. ZmSCR1 and ZmSCR1h transcripts accumulate in ground meristem cells of developing leaf primordia and in Zmscr1;Zmscr1h mutant leaves, most veins are separated by one rather than two mesophyll cells; many veins have sclerenchyma above and/or below instead of mesophyll cells; and supernumerary bundle sheath cells develop. The mutant defects are unified by compromised mesophyll cell development. In addition to Kranz defects, Zmscr1;Zmscr1h mutants fail to form an organized endodermal layer in the root. Collectively, these data indicate that ZmSCR1 and ZmSCR1h redundantly regulate cell-type patterning in both the leaves and roots of maize. Leaf and root pathways are distinguished, however, by the cell layer in which they operate – mesophyll at a two-cell distance from leaf veins versus endodermis immediately adjacent to root vasculature.
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spelling pubmed-66793602019-08-27 Redundant SCARECROW genes pattern distinct cell layers in roots and leaves of maize Hughes, Thomas E. Sedelnikova, Olga V. Wu, Hao Becraft, Philip W. Langdale, Jane A. Development Research Article The highly efficient C(4) photosynthetic pathway is facilitated by ‘Kranz’ leaf anatomy. In Kranz leaves, closely spaced veins are encircled by concentric layers of photosynthetic bundle sheath (inner) and mesophyll (outer) cells. Here, we demonstrate that, in the C(4) monocot maize, Kranz patterning is regulated by redundant function of SCARECROW 1 (ZmSCR1) and a previously uncharacterized homeologue: ZmSCR1h. ZmSCR1 and ZmSCR1h transcripts accumulate in ground meristem cells of developing leaf primordia and in Zmscr1;Zmscr1h mutant leaves, most veins are separated by one rather than two mesophyll cells; many veins have sclerenchyma above and/or below instead of mesophyll cells; and supernumerary bundle sheath cells develop. The mutant defects are unified by compromised mesophyll cell development. In addition to Kranz defects, Zmscr1;Zmscr1h mutants fail to form an organized endodermal layer in the root. Collectively, these data indicate that ZmSCR1 and ZmSCR1h redundantly regulate cell-type patterning in both the leaves and roots of maize. Leaf and root pathways are distinguished, however, by the cell layer in which they operate – mesophyll at a two-cell distance from leaf veins versus endodermis immediately adjacent to root vasculature. The Company of Biologists Ltd 2019-07-15 2019-07-19 /pmc/articles/PMC6679360/ /pubmed/31235633 http://dx.doi.org/10.1242/dev.177543 Text en © 2019. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/4.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Article
Hughes, Thomas E.
Sedelnikova, Olga V.
Wu, Hao
Becraft, Philip W.
Langdale, Jane A.
Redundant SCARECROW genes pattern distinct cell layers in roots and leaves of maize
title Redundant SCARECROW genes pattern distinct cell layers in roots and leaves of maize
title_full Redundant SCARECROW genes pattern distinct cell layers in roots and leaves of maize
title_fullStr Redundant SCARECROW genes pattern distinct cell layers in roots and leaves of maize
title_full_unstemmed Redundant SCARECROW genes pattern distinct cell layers in roots and leaves of maize
title_short Redundant SCARECROW genes pattern distinct cell layers in roots and leaves of maize
title_sort redundant scarecrow genes pattern distinct cell layers in roots and leaves of maize
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6679360/
https://www.ncbi.nlm.nih.gov/pubmed/31235633
http://dx.doi.org/10.1242/dev.177543
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