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Golgi/plastid‐type manganese superoxide dismutase involved in heat‐stress tolerance during grain filling of rice

Superoxide dismutase (SOD) is widely assumed to play a role in the detoxification of reactive oxygen species caused by environmental stresses. We found a characteristic expression of manganese SOD 1 (MSD1) in a heat‐stress‐tolerant cultivar of rice (Oryza sativa). The deduced amino acid sequence con...

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Autores principales: Shiraya, Takeshi, Mori, Taiki, Maruyama, Tatsuya, Sasaki, Maiko, Takamatsu, Takeshi, Oikawa, Kazusato, Itoh, Kimiko, Kaneko, Kentaro, Ichikawa, Hiroaki, Mitsui, Toshiaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6680209/
https://www.ncbi.nlm.nih.gov/pubmed/25586098
http://dx.doi.org/10.1111/pbi.12314
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author Shiraya, Takeshi
Mori, Taiki
Maruyama, Tatsuya
Sasaki, Maiko
Takamatsu, Takeshi
Oikawa, Kazusato
Itoh, Kimiko
Kaneko, Kentaro
Ichikawa, Hiroaki
Mitsui, Toshiaki
author_facet Shiraya, Takeshi
Mori, Taiki
Maruyama, Tatsuya
Sasaki, Maiko
Takamatsu, Takeshi
Oikawa, Kazusato
Itoh, Kimiko
Kaneko, Kentaro
Ichikawa, Hiroaki
Mitsui, Toshiaki
author_sort Shiraya, Takeshi
collection PubMed
description Superoxide dismutase (SOD) is widely assumed to play a role in the detoxification of reactive oxygen species caused by environmental stresses. We found a characteristic expression of manganese SOD 1 (MSD1) in a heat‐stress‐tolerant cultivar of rice (Oryza sativa). The deduced amino acid sequence contains a signal sequence and an N‐glycosylation site. Confocal imaging analysis of rice and onion cells transiently expressing MSD1‐YFP showed MSD1‐YFP in the Golgi apparatus and plastids, indicating that MSD1 is a unique Golgi/plastid‐type SOD. To evaluate the involvement of MSD1 in heat‐stress tolerance, we generated transgenic rice plants with either constitutive high expression or suppression of MSD1. The grain quality of rice with constitutive high expression of MSD1 grown at 33/28 °C, 12/12 h, was significantly better than that of the wild type. In contrast, MSD1‐knock‐down rice was markedly susceptible to heat stress. Quantitative shotgun proteomic analysis indicated that the overexpression of MSD1 up‐regulated reactive oxygen scavenging, chaperone and quality control systems in rice grains under heat stress. We propose that the Golgi/plastid MSD1 plays an important role in adaptation to heat stress.
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spelling pubmed-66802092019-08-09 Golgi/plastid‐type manganese superoxide dismutase involved in heat‐stress tolerance during grain filling of rice Shiraya, Takeshi Mori, Taiki Maruyama, Tatsuya Sasaki, Maiko Takamatsu, Takeshi Oikawa, Kazusato Itoh, Kimiko Kaneko, Kentaro Ichikawa, Hiroaki Mitsui, Toshiaki Plant Biotechnol J Research Articles Superoxide dismutase (SOD) is widely assumed to play a role in the detoxification of reactive oxygen species caused by environmental stresses. We found a characteristic expression of manganese SOD 1 (MSD1) in a heat‐stress‐tolerant cultivar of rice (Oryza sativa). The deduced amino acid sequence contains a signal sequence and an N‐glycosylation site. Confocal imaging analysis of rice and onion cells transiently expressing MSD1‐YFP showed MSD1‐YFP in the Golgi apparatus and plastids, indicating that MSD1 is a unique Golgi/plastid‐type SOD. To evaluate the involvement of MSD1 in heat‐stress tolerance, we generated transgenic rice plants with either constitutive high expression or suppression of MSD1. The grain quality of rice with constitutive high expression of MSD1 grown at 33/28 °C, 12/12 h, was significantly better than that of the wild type. In contrast, MSD1‐knock‐down rice was markedly susceptible to heat stress. Quantitative shotgun proteomic analysis indicated that the overexpression of MSD1 up‐regulated reactive oxygen scavenging, chaperone and quality control systems in rice grains under heat stress. We propose that the Golgi/plastid MSD1 plays an important role in adaptation to heat stress. John Wiley and Sons Inc. 2015-01-14 2015-12 /pmc/articles/PMC6680209/ /pubmed/25586098 http://dx.doi.org/10.1111/pbi.12314 Text en © 2015 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Research Articles
Shiraya, Takeshi
Mori, Taiki
Maruyama, Tatsuya
Sasaki, Maiko
Takamatsu, Takeshi
Oikawa, Kazusato
Itoh, Kimiko
Kaneko, Kentaro
Ichikawa, Hiroaki
Mitsui, Toshiaki
Golgi/plastid‐type manganese superoxide dismutase involved in heat‐stress tolerance during grain filling of rice
title Golgi/plastid‐type manganese superoxide dismutase involved in heat‐stress tolerance during grain filling of rice
title_full Golgi/plastid‐type manganese superoxide dismutase involved in heat‐stress tolerance during grain filling of rice
title_fullStr Golgi/plastid‐type manganese superoxide dismutase involved in heat‐stress tolerance during grain filling of rice
title_full_unstemmed Golgi/plastid‐type manganese superoxide dismutase involved in heat‐stress tolerance during grain filling of rice
title_short Golgi/plastid‐type manganese superoxide dismutase involved in heat‐stress tolerance during grain filling of rice
title_sort golgi/plastid‐type manganese superoxide dismutase involved in heat‐stress tolerance during grain filling of rice
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6680209/
https://www.ncbi.nlm.nih.gov/pubmed/25586098
http://dx.doi.org/10.1111/pbi.12314
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