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Nitrogen remobilization and conservation, and underlying senescence‐associated gene expression in the perennial switchgrass Panicum virgatum

Improving nitrogen (N) remobilization from aboveground to underground organs during yearly shoot senescence is an important goal for sustainable production of switchgrass (Panicum virgatum) as a biofuel crop. Little is known about the genetic control of senescence and N use efficiency in perennial g...

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Autores principales: Yang, Jiading, Worley, Eric, Ma, Qin, Li, Jun, Torres‐Jerez, Ivone, Li, Gaoyang, Zhao, Patrick X., Xu, Ying, Tang, Yuhong, Udvardi, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6680227/
https://www.ncbi.nlm.nih.gov/pubmed/26935010
http://dx.doi.org/10.1111/nph.13898
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author Yang, Jiading
Worley, Eric
Ma, Qin
Li, Jun
Torres‐Jerez, Ivone
Li, Gaoyang
Zhao, Patrick X.
Xu, Ying
Tang, Yuhong
Udvardi, Michael
author_facet Yang, Jiading
Worley, Eric
Ma, Qin
Li, Jun
Torres‐Jerez, Ivone
Li, Gaoyang
Zhao, Patrick X.
Xu, Ying
Tang, Yuhong
Udvardi, Michael
author_sort Yang, Jiading
collection PubMed
description Improving nitrogen (N) remobilization from aboveground to underground organs during yearly shoot senescence is an important goal for sustainable production of switchgrass (Panicum virgatum) as a biofuel crop. Little is known about the genetic control of senescence and N use efficiency in perennial grasses such as switchgrass, which limits our ability to improve the process. Switchgrass aboveground organs (leaves, stems and inflorescences) and underground organs (crowns and roots) were harvested every month over a 3‐yr period. Transcriptome analysis was performed to identify genes differentially expressed in various organs during development. Total N content in aboveground organs increased from spring until the end of summer, then decreased concomitant with senescence, while N content in underground organs exhibited an increase roughly matching the decrease in shoot N during fall. Hundreds of senescence‐associated genes were identified in leaves and stems. Functional grouping indicated that regulation of transcription and protein degradation play important roles in shoot senescence. Coexpression networks predict important roles for five switchgrass NAC (NAM, ATAF1,2, CUC2) transcription factors (TFs) and other TF family members in orchestrating metabolism of carbohydrates, N and lipids, protein modification/degradation, and transport processes during senescence. This study establishes a molecular basis for understanding and enhancing N remobilization and conservation in switchgrass.
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spelling pubmed-66802272019-08-09 Nitrogen remobilization and conservation, and underlying senescence‐associated gene expression in the perennial switchgrass Panicum virgatum Yang, Jiading Worley, Eric Ma, Qin Li, Jun Torres‐Jerez, Ivone Li, Gaoyang Zhao, Patrick X. Xu, Ying Tang, Yuhong Udvardi, Michael New Phytol Research Improving nitrogen (N) remobilization from aboveground to underground organs during yearly shoot senescence is an important goal for sustainable production of switchgrass (Panicum virgatum) as a biofuel crop. Little is known about the genetic control of senescence and N use efficiency in perennial grasses such as switchgrass, which limits our ability to improve the process. Switchgrass aboveground organs (leaves, stems and inflorescences) and underground organs (crowns and roots) were harvested every month over a 3‐yr period. Transcriptome analysis was performed to identify genes differentially expressed in various organs during development. Total N content in aboveground organs increased from spring until the end of summer, then decreased concomitant with senescence, while N content in underground organs exhibited an increase roughly matching the decrease in shoot N during fall. Hundreds of senescence‐associated genes were identified in leaves and stems. Functional grouping indicated that regulation of transcription and protein degradation play important roles in shoot senescence. Coexpression networks predict important roles for five switchgrass NAC (NAM, ATAF1,2, CUC2) transcription factors (TFs) and other TF family members in orchestrating metabolism of carbohydrates, N and lipids, protein modification/degradation, and transport processes during senescence. This study establishes a molecular basis for understanding and enhancing N remobilization and conservation in switchgrass. John Wiley and Sons Inc. 2016-03-03 2016-07 /pmc/articles/PMC6680227/ /pubmed/26935010 http://dx.doi.org/10.1111/nph.13898 Text en © 2016 The Authors. New Phytologist © 2016 New Phytologist Trust This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Yang, Jiading
Worley, Eric
Ma, Qin
Li, Jun
Torres‐Jerez, Ivone
Li, Gaoyang
Zhao, Patrick X.
Xu, Ying
Tang, Yuhong
Udvardi, Michael
Nitrogen remobilization and conservation, and underlying senescence‐associated gene expression in the perennial switchgrass Panicum virgatum
title Nitrogen remobilization and conservation, and underlying senescence‐associated gene expression in the perennial switchgrass Panicum virgatum
title_full Nitrogen remobilization and conservation, and underlying senescence‐associated gene expression in the perennial switchgrass Panicum virgatum
title_fullStr Nitrogen remobilization and conservation, and underlying senescence‐associated gene expression in the perennial switchgrass Panicum virgatum
title_full_unstemmed Nitrogen remobilization and conservation, and underlying senescence‐associated gene expression in the perennial switchgrass Panicum virgatum
title_short Nitrogen remobilization and conservation, and underlying senescence‐associated gene expression in the perennial switchgrass Panicum virgatum
title_sort nitrogen remobilization and conservation, and underlying senescence‐associated gene expression in the perennial switchgrass panicum virgatum
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6680227/
https://www.ncbi.nlm.nih.gov/pubmed/26935010
http://dx.doi.org/10.1111/nph.13898
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