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Teaching bioprocess engineering to undergraduates: Multidisciplinary hands‐on training in a one‐week practical course
Bioprocess engineering is a highly interdisciplinary field of study which is strongly benefited by practical courses where students can actively experience the interconnection between biology, engineering, and physical sciences. This work describes a lab course developed for 2nd year undergraduate s...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6680437/ https://www.ncbi.nlm.nih.gov/pubmed/25820495 http://dx.doi.org/10.1002/bmb.20860 |
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author | Henkel, Marius Zwick, Michaela Beuker, Janina Willenbacher, Judit Baumann, Sandra Oswald, Florian Neumann, Anke Siemann‐Herzberg, Martin Syldatk, Christoph Hausmann, Rudolf |
author_facet | Henkel, Marius Zwick, Michaela Beuker, Janina Willenbacher, Judit Baumann, Sandra Oswald, Florian Neumann, Anke Siemann‐Herzberg, Martin Syldatk, Christoph Hausmann, Rudolf |
author_sort | Henkel, Marius |
collection | PubMed |
description | Bioprocess engineering is a highly interdisciplinary field of study which is strongly benefited by practical courses where students can actively experience the interconnection between biology, engineering, and physical sciences. This work describes a lab course developed for 2nd year undergraduate students of bioprocess engineering and related disciplines, where students are challenged with a real‐life bioprocess‐engineering application, the production of recombinant protein in a fed‐batch process. The lab course was designed to introduce students to the subject of operating and supervising an experiment in a bioreactor, along with the analysis of collected data and a final critical evaluation of the experiment. To provide visual feedback of the experimental outcome, the organism used during class was Escherichia coli which carried a plasmid to recombinantly produce enhanced green fluorescent protein (eGFP) upon induction. This can easily be visualized in both the bioreactor and samples by using ultraviolet light. The lab course is performed with bioreactors of the simplest design, and is therefore highly flexible, robust and easy to reproduce. As part of this work the implementation and framework, the results, the evaluation and assessment of student learning combined with opinion surveys are presented, which provides a basis for instructors intending to implement a similar lab course at their respective institution. © 2015 by the International Union of Biochemistry and Molecular Biology, 43(3):189–202, 2015. |
format | Online Article Text |
id | pubmed-6680437 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-66804372019-08-09 Teaching bioprocess engineering to undergraduates: Multidisciplinary hands‐on training in a one‐week practical course Henkel, Marius Zwick, Michaela Beuker, Janina Willenbacher, Judit Baumann, Sandra Oswald, Florian Neumann, Anke Siemann‐Herzberg, Martin Syldatk, Christoph Hausmann, Rudolf Biochem Mol Biol Educ Laboratory Exercise Bioprocess engineering is a highly interdisciplinary field of study which is strongly benefited by practical courses where students can actively experience the interconnection between biology, engineering, and physical sciences. This work describes a lab course developed for 2nd year undergraduate students of bioprocess engineering and related disciplines, where students are challenged with a real‐life bioprocess‐engineering application, the production of recombinant protein in a fed‐batch process. The lab course was designed to introduce students to the subject of operating and supervising an experiment in a bioreactor, along with the analysis of collected data and a final critical evaluation of the experiment. To provide visual feedback of the experimental outcome, the organism used during class was Escherichia coli which carried a plasmid to recombinantly produce enhanced green fluorescent protein (eGFP) upon induction. This can easily be visualized in both the bioreactor and samples by using ultraviolet light. The lab course is performed with bioreactors of the simplest design, and is therefore highly flexible, robust and easy to reproduce. As part of this work the implementation and framework, the results, the evaluation and assessment of student learning combined with opinion surveys are presented, which provides a basis for instructors intending to implement a similar lab course at their respective institution. © 2015 by the International Union of Biochemistry and Molecular Biology, 43(3):189–202, 2015. John Wiley and Sons Inc. 2015-03-27 2015 /pmc/articles/PMC6680437/ /pubmed/25820495 http://dx.doi.org/10.1002/bmb.20860 Text en © 2015 The Authors Biochemistry and Molecular Biology Education published by Wiley Periodicals, Inc. on behalf of International Union of Biochemistry and Molecular Biology This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Laboratory Exercise Henkel, Marius Zwick, Michaela Beuker, Janina Willenbacher, Judit Baumann, Sandra Oswald, Florian Neumann, Anke Siemann‐Herzberg, Martin Syldatk, Christoph Hausmann, Rudolf Teaching bioprocess engineering to undergraduates: Multidisciplinary hands‐on training in a one‐week practical course |
title | Teaching bioprocess engineering to undergraduates: Multidisciplinary hands‐on training in a one‐week practical course |
title_full | Teaching bioprocess engineering to undergraduates: Multidisciplinary hands‐on training in a one‐week practical course |
title_fullStr | Teaching bioprocess engineering to undergraduates: Multidisciplinary hands‐on training in a one‐week practical course |
title_full_unstemmed | Teaching bioprocess engineering to undergraduates: Multidisciplinary hands‐on training in a one‐week practical course |
title_short | Teaching bioprocess engineering to undergraduates: Multidisciplinary hands‐on training in a one‐week practical course |
title_sort | teaching bioprocess engineering to undergraduates: multidisciplinary hands‐on training in a one‐week practical course |
topic | Laboratory Exercise |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6680437/ https://www.ncbi.nlm.nih.gov/pubmed/25820495 http://dx.doi.org/10.1002/bmb.20860 |
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