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Phenol-Rich Feijoa sellowiana (Pineapple Guava) Extracts Protect Human Red Blood Cells from Mercury-Induced Cellular Toxicity

Plant polyphenols, with broadly known antioxidant properties, represent very effective agents against environmental oxidative stressors, including mercury. This heavy metal irreversibly binds thiol groups, sequestering endogenous antioxidants, such as glutathione. Increased incidence of food-derived...

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Autores principales: Tortora, Fabiana, Notariale, Rosaria, Maresca, Viviana, Good, Katrina Vanessa, Sorbo, Sergio, Basile, Adriana, Piscopo, Marina, Manna, Caterina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6680458/
https://www.ncbi.nlm.nih.gov/pubmed/31336755
http://dx.doi.org/10.3390/antiox8070220
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author Tortora, Fabiana
Notariale, Rosaria
Maresca, Viviana
Good, Katrina Vanessa
Sorbo, Sergio
Basile, Adriana
Piscopo, Marina
Manna, Caterina
author_facet Tortora, Fabiana
Notariale, Rosaria
Maresca, Viviana
Good, Katrina Vanessa
Sorbo, Sergio
Basile, Adriana
Piscopo, Marina
Manna, Caterina
author_sort Tortora, Fabiana
collection PubMed
description Plant polyphenols, with broadly known antioxidant properties, represent very effective agents against environmental oxidative stressors, including mercury. This heavy metal irreversibly binds thiol groups, sequestering endogenous antioxidants, such as glutathione. Increased incidence of food-derived mercury is cause for concern, given the many severe downstream effects, ranging from kidney to cardiovascular diseases. Therefore, the possible beneficial properties of Feijoa sellowiana against mercury toxicity were tested using intact human red blood cells (RBC) incubated in the presence of HgCl(2). Here, we show that phenol-rich (10–200 µg/mL) extracts from the Feijoa sellowiana fruit potently protect against mercury-induced toxicity and oxidative stress. Peel and pulp extracts are both able to counteract the oxidative stress and thiol decrease induced in RBC by mercury treatment. Nonetheless, the peel extract had a greater protective effect compared to the pulp, although to a different extent for the different markers analyzed, which is at least partially due to the greater proportion and diversity of polyphenols in the peel. Furthermore, Fejioa sellowiana extracts also prevent mercury-induced morphological changes, which are known to enhance the pro-coagulant activity of these cells. These novel findings provide biochemical bases for the pharmacological use of Fejioa sellowiana-based functional foods in preventing and combating mercury-related illnesses.
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spelling pubmed-66804582019-08-09 Phenol-Rich Feijoa sellowiana (Pineapple Guava) Extracts Protect Human Red Blood Cells from Mercury-Induced Cellular Toxicity Tortora, Fabiana Notariale, Rosaria Maresca, Viviana Good, Katrina Vanessa Sorbo, Sergio Basile, Adriana Piscopo, Marina Manna, Caterina Antioxidants (Basel) Article Plant polyphenols, with broadly known antioxidant properties, represent very effective agents against environmental oxidative stressors, including mercury. This heavy metal irreversibly binds thiol groups, sequestering endogenous antioxidants, such as glutathione. Increased incidence of food-derived mercury is cause for concern, given the many severe downstream effects, ranging from kidney to cardiovascular diseases. Therefore, the possible beneficial properties of Feijoa sellowiana against mercury toxicity were tested using intact human red blood cells (RBC) incubated in the presence of HgCl(2). Here, we show that phenol-rich (10–200 µg/mL) extracts from the Feijoa sellowiana fruit potently protect against mercury-induced toxicity and oxidative stress. Peel and pulp extracts are both able to counteract the oxidative stress and thiol decrease induced in RBC by mercury treatment. Nonetheless, the peel extract had a greater protective effect compared to the pulp, although to a different extent for the different markers analyzed, which is at least partially due to the greater proportion and diversity of polyphenols in the peel. Furthermore, Fejioa sellowiana extracts also prevent mercury-induced morphological changes, which are known to enhance the pro-coagulant activity of these cells. These novel findings provide biochemical bases for the pharmacological use of Fejioa sellowiana-based functional foods in preventing and combating mercury-related illnesses. MDPI 2019-07-11 /pmc/articles/PMC6680458/ /pubmed/31336755 http://dx.doi.org/10.3390/antiox8070220 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tortora, Fabiana
Notariale, Rosaria
Maresca, Viviana
Good, Katrina Vanessa
Sorbo, Sergio
Basile, Adriana
Piscopo, Marina
Manna, Caterina
Phenol-Rich Feijoa sellowiana (Pineapple Guava) Extracts Protect Human Red Blood Cells from Mercury-Induced Cellular Toxicity
title Phenol-Rich Feijoa sellowiana (Pineapple Guava) Extracts Protect Human Red Blood Cells from Mercury-Induced Cellular Toxicity
title_full Phenol-Rich Feijoa sellowiana (Pineapple Guava) Extracts Protect Human Red Blood Cells from Mercury-Induced Cellular Toxicity
title_fullStr Phenol-Rich Feijoa sellowiana (Pineapple Guava) Extracts Protect Human Red Blood Cells from Mercury-Induced Cellular Toxicity
title_full_unstemmed Phenol-Rich Feijoa sellowiana (Pineapple Guava) Extracts Protect Human Red Blood Cells from Mercury-Induced Cellular Toxicity
title_short Phenol-Rich Feijoa sellowiana (Pineapple Guava) Extracts Protect Human Red Blood Cells from Mercury-Induced Cellular Toxicity
title_sort phenol-rich feijoa sellowiana (pineapple guava) extracts protect human red blood cells from mercury-induced cellular toxicity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6680458/
https://www.ncbi.nlm.nih.gov/pubmed/31336755
http://dx.doi.org/10.3390/antiox8070220
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