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Adsorption of Vi Capsular Antigen of Salmonella Typhi in Chitosan–Poly (Methacrylic Acid) Nanoparticles

The development of a nanoparticulate system for the carrier antigen is now an important tool in the vaccination process, since a smaller number of doses is necessary for effective immunization. Thus, in this work a nanoparticulate system using polymers of chitosan and poly (methacrylic acid) (CS–PMA...

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Autores principales: da Silva, Raimundo Lopes, da Silva, Jaqueline Rodrigues, Júnior, Anivaldo Pereira Duarte, Marinho, Patrícia Santana Barbosa, Santos, Lourivaldo Silva, Teixeira, Francisco Martins, Júnior, José Otávio Carréra Silva, Costa, Roseane Maria Ribeiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6680519/
https://www.ncbi.nlm.nih.gov/pubmed/31340432
http://dx.doi.org/10.3390/polym11071226
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author da Silva, Raimundo Lopes
da Silva, Jaqueline Rodrigues
Júnior, Anivaldo Pereira Duarte
Marinho, Patrícia Santana Barbosa
Santos, Lourivaldo Silva
Teixeira, Francisco Martins
Júnior, José Otávio Carréra Silva
Costa, Roseane Maria Ribeiro
author_facet da Silva, Raimundo Lopes
da Silva, Jaqueline Rodrigues
Júnior, Anivaldo Pereira Duarte
Marinho, Patrícia Santana Barbosa
Santos, Lourivaldo Silva
Teixeira, Francisco Martins
Júnior, José Otávio Carréra Silva
Costa, Roseane Maria Ribeiro
author_sort da Silva, Raimundo Lopes
collection PubMed
description The development of a nanoparticulate system for the carrier antigen is now an important tool in the vaccination process, since a smaller number of doses is necessary for effective immunization. Thus, in this work a nanoparticulate system using polymers of chitosan and poly (methacrylic acid) (CS–PMAA) to adsorb the Vi antigen of Salmonella Typhi was developed. CS–PMAA nanoparticles with different proportions of chitosan and poly (methacrylic acid) were obtained and reached sizes from 123.9 ± 2.48 to 234.9 ± 2.66 nm, and spherical shapes were seen in transmission microscopy. At pH 7.2, the nanoparticles had a cationic surface charge that contributed to the adsorption of the Vi antigen. Qualitative analyses of the isolated Vi antigen were performed using Fourier-transform infrared spectroscopy, which indicated the presence of all the characteristic bands of the capsular polysaccharide, and nuclear magnetic resonance, which showed signals for the five hydrogens and the N-acetyl and O-acetyl groups which are characteristic of the Vi antigen structure. In the adsorption kinetics study, the Vi capsular antigen, contained in a phosphate buffer solution of pH 7.2, experienced 55% adsorption on the 1–1% (CS–PMAA) nanoparticles. The adsorption kinetics results showed the ability of the nanoparticulate system to adsorb the Vi antigen.
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spelling pubmed-66805192019-08-09 Adsorption of Vi Capsular Antigen of Salmonella Typhi in Chitosan–Poly (Methacrylic Acid) Nanoparticles da Silva, Raimundo Lopes da Silva, Jaqueline Rodrigues Júnior, Anivaldo Pereira Duarte Marinho, Patrícia Santana Barbosa Santos, Lourivaldo Silva Teixeira, Francisco Martins Júnior, José Otávio Carréra Silva Costa, Roseane Maria Ribeiro Polymers (Basel) Article The development of a nanoparticulate system for the carrier antigen is now an important tool in the vaccination process, since a smaller number of doses is necessary for effective immunization. Thus, in this work a nanoparticulate system using polymers of chitosan and poly (methacrylic acid) (CS–PMAA) to adsorb the Vi antigen of Salmonella Typhi was developed. CS–PMAA nanoparticles with different proportions of chitosan and poly (methacrylic acid) were obtained and reached sizes from 123.9 ± 2.48 to 234.9 ± 2.66 nm, and spherical shapes were seen in transmission microscopy. At pH 7.2, the nanoparticles had a cationic surface charge that contributed to the adsorption of the Vi antigen. Qualitative analyses of the isolated Vi antigen were performed using Fourier-transform infrared spectroscopy, which indicated the presence of all the characteristic bands of the capsular polysaccharide, and nuclear magnetic resonance, which showed signals for the five hydrogens and the N-acetyl and O-acetyl groups which are characteristic of the Vi antigen structure. In the adsorption kinetics study, the Vi capsular antigen, contained in a phosphate buffer solution of pH 7.2, experienced 55% adsorption on the 1–1% (CS–PMAA) nanoparticles. The adsorption kinetics results showed the ability of the nanoparticulate system to adsorb the Vi antigen. MDPI 2019-07-23 /pmc/articles/PMC6680519/ /pubmed/31340432 http://dx.doi.org/10.3390/polym11071226 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
da Silva, Raimundo Lopes
da Silva, Jaqueline Rodrigues
Júnior, Anivaldo Pereira Duarte
Marinho, Patrícia Santana Barbosa
Santos, Lourivaldo Silva
Teixeira, Francisco Martins
Júnior, José Otávio Carréra Silva
Costa, Roseane Maria Ribeiro
Adsorption of Vi Capsular Antigen of Salmonella Typhi in Chitosan–Poly (Methacrylic Acid) Nanoparticles
title Adsorption of Vi Capsular Antigen of Salmonella Typhi in Chitosan–Poly (Methacrylic Acid) Nanoparticles
title_full Adsorption of Vi Capsular Antigen of Salmonella Typhi in Chitosan–Poly (Methacrylic Acid) Nanoparticles
title_fullStr Adsorption of Vi Capsular Antigen of Salmonella Typhi in Chitosan–Poly (Methacrylic Acid) Nanoparticles
title_full_unstemmed Adsorption of Vi Capsular Antigen of Salmonella Typhi in Chitosan–Poly (Methacrylic Acid) Nanoparticles
title_short Adsorption of Vi Capsular Antigen of Salmonella Typhi in Chitosan–Poly (Methacrylic Acid) Nanoparticles
title_sort adsorption of vi capsular antigen of salmonella typhi in chitosan–poly (methacrylic acid) nanoparticles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6680519/
https://www.ncbi.nlm.nih.gov/pubmed/31340432
http://dx.doi.org/10.3390/polym11071226
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