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Analysis of Bacteriophages with Insulator-Based Dielectrophoresis

Bacterial viruses or phages have great potential in the medical and agricultural fields as alternatives to antibiotics to control nuisance populations of pathogenic bacteria. However, current analysis and purification protocols for phages tend to be resource intensive and have numbers of limitations...

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Autores principales: Coll De Peña, Adriana, Mohd Redzuan, Nurul Humaira, Abajorga, Milky K., Hill, Nicole, Thomas, Julie A., Lapizco-Encinas, Blanca H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6680707/
https://www.ncbi.nlm.nih.gov/pubmed/31277396
http://dx.doi.org/10.3390/mi10070450
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author Coll De Peña, Adriana
Mohd Redzuan, Nurul Humaira
Abajorga, Milky K.
Hill, Nicole
Thomas, Julie A.
Lapizco-Encinas, Blanca H.
author_facet Coll De Peña, Adriana
Mohd Redzuan, Nurul Humaira
Abajorga, Milky K.
Hill, Nicole
Thomas, Julie A.
Lapizco-Encinas, Blanca H.
author_sort Coll De Peña, Adriana
collection PubMed
description Bacterial viruses or phages have great potential in the medical and agricultural fields as alternatives to antibiotics to control nuisance populations of pathogenic bacteria. However, current analysis and purification protocols for phages tend to be resource intensive and have numbers of limitations, such as impacting phage viability. The present study explores the potential of employing the electrokinetic technique of insulator-based dielectrophoresis (iDEP) for virus assessment, separation and enrichment. In particular, the application of the parameter “trapping value” (Tv) is explored as a standardized iDEP signature for each phage species. The present study includes mathematical modeling with COMSOL Multiphysics and extensive experimentation. Three related, but genetically and structurally distinct, phages were studied: Salmonella enterica phage SPN3US, Pseudomonas aeruginosa phage ϕKZ and P. chlororaphis phage 201ϕ2-1. This is the first iDEP study on bacteriophages with large and complex virions and the results illustrate their virions can be successfully enriched with iDEP systems and still retain infectivity. In addition, our results indicate that characterization of the negative dielectrophoretic response of a phage in terms of Tv could be used for predicting individual virus behavior in iDEP systems. The findings reported here can contribute to the establishment of protocols to analyze, purify and/or enrich samples of known and unknown phages.
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spelling pubmed-66807072019-08-09 Analysis of Bacteriophages with Insulator-Based Dielectrophoresis Coll De Peña, Adriana Mohd Redzuan, Nurul Humaira Abajorga, Milky K. Hill, Nicole Thomas, Julie A. Lapizco-Encinas, Blanca H. Micromachines (Basel) Article Bacterial viruses or phages have great potential in the medical and agricultural fields as alternatives to antibiotics to control nuisance populations of pathogenic bacteria. However, current analysis and purification protocols for phages tend to be resource intensive and have numbers of limitations, such as impacting phage viability. The present study explores the potential of employing the electrokinetic technique of insulator-based dielectrophoresis (iDEP) for virus assessment, separation and enrichment. In particular, the application of the parameter “trapping value” (Tv) is explored as a standardized iDEP signature for each phage species. The present study includes mathematical modeling with COMSOL Multiphysics and extensive experimentation. Three related, but genetically and structurally distinct, phages were studied: Salmonella enterica phage SPN3US, Pseudomonas aeruginosa phage ϕKZ and P. chlororaphis phage 201ϕ2-1. This is the first iDEP study on bacteriophages with large and complex virions and the results illustrate their virions can be successfully enriched with iDEP systems and still retain infectivity. In addition, our results indicate that characterization of the negative dielectrophoretic response of a phage in terms of Tv could be used for predicting individual virus behavior in iDEP systems. The findings reported here can contribute to the establishment of protocols to analyze, purify and/or enrich samples of known and unknown phages. MDPI 2019-07-04 /pmc/articles/PMC6680707/ /pubmed/31277396 http://dx.doi.org/10.3390/mi10070450 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Coll De Peña, Adriana
Mohd Redzuan, Nurul Humaira
Abajorga, Milky K.
Hill, Nicole
Thomas, Julie A.
Lapizco-Encinas, Blanca H.
Analysis of Bacteriophages with Insulator-Based Dielectrophoresis
title Analysis of Bacteriophages with Insulator-Based Dielectrophoresis
title_full Analysis of Bacteriophages with Insulator-Based Dielectrophoresis
title_fullStr Analysis of Bacteriophages with Insulator-Based Dielectrophoresis
title_full_unstemmed Analysis of Bacteriophages with Insulator-Based Dielectrophoresis
title_short Analysis of Bacteriophages with Insulator-Based Dielectrophoresis
title_sort analysis of bacteriophages with insulator-based dielectrophoresis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6680707/
https://www.ncbi.nlm.nih.gov/pubmed/31277396
http://dx.doi.org/10.3390/mi10070450
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